Genetic control of the rate of alpha-amylase synthesis in Bacillus subtilis.

Abstract:

:The level of extracellular alpha-amylase (EC 3.2.1.1) of Bacillus subtilis Marburg was increased about fivefold by introducing the amyR marker from B. natto 1212 through transformation. amyR2 of B. natto 1212 has been assumed to determine a high level of alpha-amylase of the organism. The gene acts specifically on alpha-amylase synthesis but not on the production of other extracellular enzymes. alpha-Amylase of an amyR2-carrying strain was found to be quite similar to that of an isogenic amyR1-carrying strain in the thermostability and electrophoretic behavior of whichever amylase the strain produces. Marburg-type alpha-amylase (amyEm) or B. natto-alpha-amylase (amyEn). Anti-amylase serum titration indicates that a high level of the enzyme activity in the amyR2-carrying strain is caused by the existence of more enzyme rather than the presence of an enzyme having higher efficiency. This is supported further by the fact that amyR controls the synthesis of the amyE gene product in mutant M9, which synthesizes a temperature-sensitive-alpha-amylase, and in mutant M07, which secretes cross-reacting material. The results indicate that amyR regulates the rate of alpha-amylase synthesis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yamaguchi K,Nagata Y,Maruo B

doi

10.1128/JB.119.2.410-415.1974

subject

Has Abstract

pub_date

1974-08-01 00:00:00

pages

410-5

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

119

pub_type

杂志文章
  • The dimerization function of MinC resides in a structurally autonomous C-terminal domain.

    abstract::Limited proteolysis of the Escherichia coli cell division inhibitor MinC reveals that its dimerization function resides in a structurally autonomous C-terminal domain. We show that cytoplasmic MinC is poised near the monomer-dimer equilibrium and propose that it only becomes entirely dimeric once recruited to the memb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.22.6684-6687.2001

    authors: Szeto TH,Rowland SL,King GF

    更新日期:2001-11-01 00:00:00

  • Evidence for two phosphonate degradative pathways in Enterobacter aerogenes.

    abstract::We screened mini-Mu plasmid libraries from Enterobacter aerogenes IFO 12010 for plasmids that complement Escherichia coli phn mutants that cannot use phosphonates (Pn) as the sole source of phosphorus (P). We isolated two kinds of plasmids that, unexpectedly, encode genes for different metabolic pathways. One kind com...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.8.2501-2510.1992

    authors: Lee KS,Metcalf WW,Wanner BL

    更新日期:1992-04-01 00:00:00

  • Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii.

    abstract::Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01952-06

    authors: Mishra A,Das A,Cisar JO,Ton-That H

    更新日期:2007-04-01 00:00:00

  • RmpA regulation of capsular polysaccharide biosynthesis in Klebsiella pneumoniae CG43.

    abstract::Sequence analysis of the large virulence plasmid pLVPK in Klebsiella pneumoniae CG43 revealed the presence of another mucoid factor encoding gene rmpA besides rmpA2. Promoter activity measurement indicated that the deletion of rmpA reduced K2 capsular polysaccharide (CPS) biosynthesis, resulting in decreased colony mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00031-10

    authors: Cheng HY,Chen YS,Wu CY,Chang HY,Lai YC,Peng HL

    更新日期:2010-06-01 00:00:00

  • Novel pathway for conversion of chlorohydroxyquinol to maleylacetate in Burkholderia cepacia AC1100.

    abstract::Burkholderia cepacia AC1100 metabolizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) via formation of 5-chlorohydroxyquinol (5-CHQ), hydroxyquinol (HQ), maleylacetate, and beta-oxoadipate. The step(s) leading to the dechlorination of 5-CHQ to HQ has remained unidentified. We demonstrate that a dechlorinating enzyme, Tf...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4667-4675.1998

    authors: Zaborina O,Daubaras DL,Zago A,Xun L,Saido K,Klem T,Nikolic D,Chakrabarty AM

    更新日期:1998-09-01 00:00:00

  • Glucose dehydrogenation in bacteria: a comparative study.

    abstract::Hauge, Jens G. (National Institute for Public Health, Oslo, Norway). Glucose dehydrogenation in bacteria: a comparative study. J. Bacteriol. 82:609-614. 1961.-Extracts of a series of bacteria reported to convert glucose to gluconic acid via particulate enzyme systems were fractionated by differential centrifugation in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.4.609-614.1961

    authors: HAUGE JG

    更新日期:1961-10-01 00:00:00

  • Isolation and characterization of an Escherichia coli seryl-tRNA synthetase mutant with a large increase in Km for serine.

    abstract::A mutant of Escherichia coli resistant to serine hydroxamate which has a large increase in Km for serine of seryl-tRNA synthetase is described. The mutant serS gene was cloned and sequenced and was found to contain a single-base-pair mutation, resulting in the substitution of the residue alanine 262 by valine in motif...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.11.3347-3350.1995

    authors: Willison JC,Härtlein M,Leberman R

    更新日期:1995-06-01 00:00:00

  • The LiaFSR system regulates the cell envelope stress response in Streptococcus mutans.

    abstract::Maintaining cell envelope integrity is critical for bacterial survival, including bacteria living in a complex and dynamic environment such as the human oral cavity. Streptococcus mutans, a major etiological agent of dental caries, uses two-component signal transduction systems (TCSTSs) to monitor and respond to vario...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01563-08

    authors: Suntharalingam P,Senadheera MD,Mair RW,Lévesque CM,Cvitkovitch DG

    更新日期:2009-05-01 00:00:00

  • PSITTACOSIS-INFECTED CELL SYSTEM FOR STUDIES ON RADIATION DAMAGE.

    abstract::Sharon, Nehama (University of Notre Dame, Notre Dame, Ind.), Morris Pollard, and W. R. Klemm. Psittacosis-infected cell system for studies on radiation damage. J. Bacteriol. 86:1139-1144. 1963.-The unique replication pattern of the agent of psittacosis in cells provides a means for studying biological effects of radia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.6.1139-1144.1963

    authors: SHARON N,POLLARD M,KLEMM WR

    更新日期:1963-12-01 00:00:00

  • The proton motive force generated in Leuconostoc oenos by L-malate fermentation.

    abstract::In cells of Leuconostoc oenos, the fermentation of L-malic acid generates both a transmembrane pH gradient, inside alkaline, and an electrical potential gradient, inside negative. In resting cells, the proton motive force ranged from -170 mV to -88 mV between pH 3.1 and 5.6 in the presence Of L-malate. Membrane potent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3127-3132.1996

    authors: Salema M,Lolkema JS,San Romão MV,Lourero Dias MC

    更新日期:1996-06-01 00:00:00

  • New solid medium for enhanced growth of Pasteurella tularensis.

    abstract::Gaspar, Andrew J. (Fort Detrick, Frederick, Md.), Hugh B. Tresselt, and Martha K. Ward. New solid medium for enhanced growth of Pasteurella tularensis. J. Bacteriol. 82:564-569. 1961.-The purpose of this work was to develop a solid medium for more rapid growth of Pasteurella tularensis, especially from small inocula c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.4.564-569.1961

    authors: GASPAR AJ,TRESSELT HB,WARD MK

    更新日期:1961-10-01 00:00:00

  • Effects of a mutation that eliminates UDP glucose-pyrophosphorylase on the pathogenicity of Erwinia carotovora subsp. carotovora.

    abstract::A nonpathogenic mutant of Erwinia carotovora obtained by Mu d1 mutagenesis was defective in the ability to utilize several carbon sources. The basis of the mutation was analyzed biochemically and shown to be a defect in the ability to form UDP glucose-pyrophosphorylase. The nonpathogenic phenotype of the mutant was ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.473-476.1985

    authors: Jayaswal RK,Bressan RA,Handa AK

    更新日期:1985-10-01 00:00:00

  • Structure and composition of the cell wall of Neurospora crassa.

    abstract::The structure and composition of the cell walls of hyphae of Neurospora crassa were investigated by electron microscopy, chemical analysis, and X-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. The wall consists of two phases: randomly disposed skel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.202-212.1967

    authors: Manocha MS,Colvin JR

    更新日期:1967-07-01 00:00:00

  • Inhibition of thiamine transport by chloroethylthiamine in Escherichia coli.

    abstract::Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1438-1440.1972

    authors: Iwashima A,Nose Y

    更新日期:1972-12-01 00:00:00

  • Transcriptional analysis and mutation of a dnaA-like gene in Synechocystis sp. strain PCC 6803.

    abstract::Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and di...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.18.4946-4949.1998

    authors: Richter S,Hagemann M,Messer W

    更新日期:1998-09-01 00:00:00

  • Conditional synthesis and utilization of 1,5-anhydroglucitol in Escherichia coli.

    abstract::A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.22.7138-7141.1993

    authors: Shiga Y,Mizuno H,Akanuma H

    更新日期:1993-11-01 00:00:00

  • Purification, properties, and sequence of glycerol trinitrate reductase from Agrobacterium radiobacter.

    abstract::Glycerol trinitrate (GTN) reductase, which enables Agrobacterium radiobacter to utilize GTN and related explosives as sources of nitrogen for growth, was purified and characterized, and its gene was cloned and sequenced. The enzyme was a 39-kDa monomeric protein which catalyzed the NADH-dependent reductive scission of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.24.7796-7802.1997

    authors: Snape JR,Walkley NA,Morby AP,Nicklin S,White GF

    更新日期:1997-12-01 00:00:00

  • Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR-amplified rRNA genes.

    abstract::Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.4.973-981.1993

    authors: Ralph D,McClelland M,Welsh J,Baranton G,Perolat P

    更新日期:1993-02-01 00:00:00

  • Enhanced acetohydroxy acid synthase III activity in an ilvH mutant of Escherichia coli K-12.

    abstract::The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5197-5199.1988

    authors: Ricca E,Limauro D,Lago CT,de Felice M

    更新日期:1988-11-01 00:00:00

  • Purification, characterization, and regulation of a nicotinamide adenine dinucleotide-dependent lactate dehydrogenase from Actinomyces viscosus.

    abstract::A nicotinamide adenine dinucleotide-specific L-(+)-lactate dehydrogenase (LDH) (EC 1.11.27) from Actinomyces viscosus T-6-1600 was purified approximately 110-fold by a combination of diethylaminoethyl-cellulose and 0.5 M Agarose A column chromatography. The ldh was stable at 26 C, but was quite labile at temperatures ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.1126-1135.1975

    authors: Brown AT,Christian CP,Eifert RL

    更新日期:1975-06-01 00:00:00

  • Molecular mapping of glyW, a duplicate gene for tRNA3Gly of Escherichia coli.

    abstract::By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Tucker SD,Gopalakrishnan AS,Bollinger R,Dowhan W,Murgola EJ

    更新日期:1982-11-01 00:00:00

  • Resistance to arsenic compounds conferred by a plasmid transmissible between strains of Escherichia coli.

    abstract::A plasmid, R773, which confers resistance to arsenic compounds, is transmissible between strains of Escherichia coli. It is a member of compatibility group F(1). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.459-460.1973

    authors: Hedges RW,Baumberg S

    更新日期:1973-07-01 00:00:00

  • Aeromonas hydrophila lateral flagellar gene transcriptional hierarchy.

    abstract::Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many impor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01994-12

    authors: Wilhelms M,Gonzalez V,Tomás JM,Merino S

    更新日期:2013-04-01 00:00:00

  • Subunit interactions and protein stability in the cyanobacterial light-harvesting proteins.

    abstract::Strain 4R is a phycocyanin-minus mutant of the unicellular cyanobacterium Synechocystis sp. strain 6803. Although it lacks the light-harvesting protein phycocyanin, 4R has normal levels of phycocyanin (cpc) transcripts. Sequence analysis of the cpcB gene encoding the phycocyanin beta subunit shows an insertion mutatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.23.6798-6803.1995

    authors: Plank T,Toole C,Anderson LK

    更新日期:1995-12-01 00:00:00

  • The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system.

    abstract::The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.14.4520-4527.1993

    authors: Rathsam C,Giffard PM,Jacques NA

    更新日期:1993-07-01 00:00:00

  • Influences of growth substrates and oxygen on the electron transport system in Acinetobacter sp. HO1-N.

    abstract::The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various concentrations of oxygen or types of growth substrates. Spectrophotometric analysis revealed that the quantity and type...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.3.859-868.1980

    authors: Ensley BD Jr,Finnerty WR

    更新日期:1980-06-01 00:00:00

  • Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors.

    abstract::Three Escherichia coli phages, TuIa, TuIb, and TuII, were isolated from local sewage. We present evidence that they use the major outer membrane proteins Ia, Ib, and II, respectively, as receptors. In all cases the proteins, under the experimental conditions used, required lipopolysaccharide to exhibit their receptor ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.3.821-829.1977

    authors: Datta DB,Arden B,Henning U

    更新日期:1977-09-01 00:00:00

  • Role of the Helicobacter hepaticus flagellar sigma factor FliA in gene regulation and murine colonization.

    abstract::The enterohepatic Helicobacter species Helicobacter hepaticus colonizes the murine intestinal and hepatobiliary tract and is associated with chronic intestinal inflammation, gall stone formation, hepatitis, and hepatocellular carcinoma. Thus far, the role of H. hepaticus motility and flagella in intestinal colonizatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00626-08

    authors: Sterzenbach T,Bartonickova L,Behrens W,Brenneke B,Schulze J,Kops F,Chin EY,Katzowitsch E,Schauer DB,Fox JG,Suerbaum S,Josenhans C

    更新日期:2008-10-01 00:00:00

  • Rhizobium meliloti insertion element ISRm2 and its use for identification of the fixX gene.

    abstract::Two of the three plasmids of the wild-type Rhizobium meliloti 41 (pRme41a and pRme41c) carry a copy of ISRm2, a 2.7-kilobase-long transposable element. ISRm2 is terminated by 22-base-pair (bp) inverted repeat sequences, exhibiting some homology to the inverted repeats of elements generating 9-bp target sequence duplic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.4.1403-1409.1987

    authors: Dusha I,Kovalenko S,Banfalvi Z,Kondorosi A

    更新日期:1987-04-01 00:00:00

  • Porphyrin binding by the surface array virulence protein of Aeromonas salmonicida.

    abstract::Congo red binding by virulent A-layer-containing (A+) and avirulent A-layer-deficient (A-) strains of Aeromonas salmonicida was examined. Congo red binding to A+ cells was enhanced by salt and thus hydrophobically driven, but at low Congo red concentrations binding was salt independent. Congo red was bound by A+ cells...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1332-1336.1985

    authors: Kay WW,Phipps BM,Ishiguro EE,Trust TJ

    更新日期:1985-12-01 00:00:00