Aeromonas hydrophila lateral flagellar gene transcriptional hierarchy.

Abstract:

:Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many important differences from, genes of Vibrio parahaemolyticus. A. hydrophila lateral flagellum class I gene transcription is σ(70) dependent, which is consistent with the fact that lateral flagellum is constitutively transcribed, in contrast to the characteristics of V. parahaemolyticus. The fact that multiple genes are included in class I highlights that lateral flagellar genes are less hierarchically transcribed than polar flagellum genes. The A. hydrophila lafK-fliEJL gene cluster (where the subscript L distinguishes genes for lateral flagella from those for polar flagella) is exclusively from class I and is in V. parahaemolyticus class I and II. Furthermore, the A. hydrophila flgAMNL cluster is not transcribed from the σ(54)/LafK-dependent promoter and does not contain class II genes. Here, we propose a gene transcriptional hierarchy for the A. hydrophila lateral flagella.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wilhelms M,Gonzalez V,Tomás JM,Merino S

doi

10.1128/JB.01994-12

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

1436-45

issue

7

eissn

0021-9193

issn

1098-5530

pii

JB.01994-12

journal_volume

195

pub_type

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