Purification and activation properties of UreD-UreF-urease apoprotein complexes.

Abstract:

:In vivo assembly of the Klebsiella aerogenes urease nickel metallocenter requires the presence of UreD, UreF, and UreG accessory proteins and is further facilitated by UreE. Prior studies had shown that urease apoprotein exists in an uncomplexed form as well as in a series of UreD-urease (I.-S. Park, M.B. Carr, and R.P. Hausinger, Proc. Natl. Acad. Sci. USA 91:3233-3237, 1994) and UreD-UreF-UreG-urease (I.-S. Park and R.P. Hausinger, J. Bacteriol. 177:1947-1951, 1995) apoprotein complexes. This study demonstrates the existence of a distinct series of complexes consisting of UreD, UreF, and urease apoprotein. These novel complexes exhibited activation properties that were distinct from urease and UreD-urease apoprotein complexes. Unlike the previously described species, the UreD-UreF-urease apoprotein complexes were resistant to inactivation by NiCl2. The bicarbonate concentration dependence for UreD-UreF-urease apoenzyme activation was significantly decreased compared with that of the urease and UreD-urease apoproteins. Western blot (immunoblot) analyses with polyclonal anti-urease and anti-UreD antibodies indicated that UreD is masked in the UreD-UreF-urease complexes, presumably by UreF. We propose that the binding of UreF modulates the UreD-urease apoprotein activation properties by excluding nickel ions from binding to the active site until after formation of the carbamylated lysine metallocenter ligand.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Moncrief MB,Hausinger RP

doi

10.1128/jb.178.18.5417-5421.1996

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

5417-21

issue

18

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.

    abstract::Peptidoglycan hydrolase, LytF (CwlE), was determined to be identical to YhdD (deduced cell wall binding protein) by zymography after insertional inactivation of the yhdD gene. YhdD exhibits high sequence similarity with CwlF (PapQ, LytE) and p60 of Listeria monocytogenes. The N-terminal region of YhdD has a signal seq...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.10.3178-3184.1999

    authors: Ohnishi R,Ishikawa S,Sekiguchi J

    更新日期:1999-05-01 00:00:00

  • Comparative ultrastructure of the thiobacilli.

    abstract::The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.2.618-627.1970

    authors: Shively JM,Decker GL,Greenawalt JW

    更新日期:1970-02-01 00:00:00

  • Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene.

    abstract::Two different 30-nucleotide regions of the cryIAc insecticidal protoxin gene from Bacillus thuringiensis were randomly mutagenized. One region was within one of seven amphipathic helices believed to be important for the formation of ion channels. There was no loss of toxicity for three test insects by any of 27 mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.14.4059-4065.1995

    authors: Aronson AI,Wu D,Zhang C

    更新日期:1995-07-01 00:00:00

  • Effects of Dimethylsulfoxide on the Lactose Operon in Escherichia coli.

    abstract::Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight ext...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.2.353-357.1966

    authors: Fowler AV,Zabin I

    更新日期:1966-08-01 00:00:00

  • Secondary and tertiary responses of the induced bactericidin from the West Indian spiny lobster, Panulirus argus.

    abstract::West Indian spiny lobsters, Panulirus argus, synthesized a hemolymph bactericidin after being injected with killed suspensions of gram-negative bacillus EMB-1 isolated from the normal gut of this lobster. To study differences between the primary response and secondary response, animals were given a primary antigen inj...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.943-946.1969

    authors: Evans EE,Weinheimer PF,Painter B,Acton RT,Evans ML

    更新日期:1969-06-01 00:00:00

  • Use of cloned DNA methylase genes to increase the frequency of transfer of foreign genes into Xanthomonas campestris pv. malvacearum.

    abstract::In vitro-packaged cosmid libraries of DNA from the bacterium Xanthomonas campestris pv. malvacearum were restricted 200- to 1,000-fold when introduced into Mcr+ strains of Escherichia coli compared with restriction in the Mcr- strain HB101. Restriction was predominantly associated with the mcrBC+ gene in E. coli. A pl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.20.6421-6427.1991

    authors: De Feyter R,Gabriel DW

    更新日期:1991-10-01 00:00:00

  • A selenium-dependent xanthine dehydrogenase triggers biofilm proliferation in Enterococcus faecalis through oxidant production.

    abstract::Selenium has been shown to be present as a labile cofactor in a small class of molybdenum hydroxylase enzymes in several species of clostridia that specialize in the fermentation of purines and pyrimidines. This labile cofactor is poorly understood, yet recent bioinformatic studies have suggested that Enterococcus fae...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01063-10

    authors: Srivastava M,Mallard C,Barke T,Hancock LE,Self WT

    更新日期:2011-04-01 00:00:00

  • Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions.

    abstract::The folded chromosome of Escherichia coli has been investigated under various lysis and physiological conditions. A new gradient system was devised that allows excellent separation between unlysed cells and envelope-associated and envelope-free chromosomes. Isotope incorporation experiments showed that the fraction of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.2.904-916.1976

    authors: Korch C,Ovrebo S,Kleppe K

    更新日期:1976-08-01 00:00:00

  • The bile response repressor BreR regulates expression of the Vibrio cholerae breAB efflux system operon.

    abstract::Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expressio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00584-08

    authors: Cerda-Maira FA,Ringelberg CS,Taylor RK

    更新日期:2008-11-01 00:00:00

  • Subunits of succinyl-coenzyme A synthetase: coordination of production in Escherichia coli and discovery of a factor that precludes refolding.

    abstract::Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.231-237.1980

    authors: Wolodko WT,Brownie ER,Bridger WA

    更新日期:1980-07-01 00:00:00

  • Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector.

    abstract::A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.3.831-836.1986

    authors: Wirth R,An FY,Clewell DB

    更新日期:1986-03-01 00:00:00

  • PHYSIOLOGICAL STATE OF ESCHERICHIA COLI AND THE INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS BY PHENETHYL ALCOHOL.

    abstract::Treick, R. W. (Indiana University, Bloomington), and W. A. Konetzka. Physiological state of Escherichia coli and the inhibition of deoxyribonucleic acid synthesis by phenethyl alcohol. J. Bacteriol. 88:1580-1584. 1964.-The effects of concentration of phenethyl alcohol (PEA) and the physiological state of the cells on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.6.1580-1584.1964

    authors: TREICK RW,KONETZKA WA

    更新日期:1964-12-01 00:00:00

  • THE GENUS VEILLONELLA. I. GENERAL CULTURAL, ECOLOGICAL, AND BIOCHEMICAL CONSIDERATIONS.

    abstract::Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.162-170.1964

    authors: ROGOSA M

    更新日期:1964-01-01 00:00:00

  • Uranyl nitrate inhibition of transport systems in Saccharomyces cerevisiae.

    abstract::Uranyl nitrate inhibited the transport of several amino acids, a vitamin, and a disaccharide into yeast. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.1205-1206.1971

    authors: Maxwell WA,Metzler R,Spoerl E

    更新日期:1971-03-01 00:00:00

  • Staphylococcus aureus Preferentially Liberates Inorganic Phosphate from Organophosphates in Environments where This Nutrient Is Limiting.

    abstract::Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00264-20

    authors: Kelliher JL,Leder Macek AJ,Grudzinski KM,Radin JN,Kehl-Fie TE

    更新日期:2020-10-22 00:00:00

  • Recombination in Escherichia coli and the definition of biological species.

    abstract::The DNA sequence of part of the gnd (6-phosphogluconate dehydrogenase) gene was determined for eight wild strains of Escherichia coli and for Salmonella typhimurium. Since a region of the trp (tryptophan) operon and the phoA (alkaline phosphatase) gene have been sequenced from the same strains, the gene trees for thes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.22.7257-7268.1991

    authors: Dykhuizen DE,Green L

    更新日期:1991-11-01 00:00:00

  • Macromolecular structure and morphology of native glycogen particles isolated from Candida albicans.

    abstract::A polysaccharide-rich particulate fraction was isolated from cytoplasmic extracts of Candida albicans by a procedure using differential centrifugation. The polysaccharide particles obtained after purification with deoxycholate treatment were essentially free of nitrogen and were identified chemically as polyglucosan, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.1.441-449.1974

    authors: Yamaguchi H,Kanda Y,Iwata K

    更新日期:1974-10-01 00:00:00

  • Distribution of cytochrome c peroxidase activity in wild-type and petite cells of bakers' yeast grown aerobically and anaerobically.

    abstract::Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.4.1225-1235.1967

    authors: Avers CJ

    更新日期:1967-10-01 00:00:00

  • Macromolecular synthesis during the germanation of Saccharomyces cerevisiae spores.

    abstract::After the dormancy of Saccharomyces cerevisiae ascospores had been broken, the synthesis of proteins was observed first, followed rapidly by synthesis of ribonucleic acid (RNA) and much later by deoxyribonucleic acid (DNA) synthesis. Phosphoglucomutase activity increased in a periodic (step) fashion, whereas the activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1289-1295.1973

    authors: Rousseau P,Halvorson HO

    更新日期:1973-03-01 00:00:00

  • Identification of a second lipopolysaccharide in Porphyromonas gingivalis W50.

    abstract::We previously described a cell surface anionic polysaccharide (APS) in Porphyromonas gingivalis that is required for cell integrity and serum resistance. APS is a phosphorylated branched mannan that shares a common epitope with posttranslational additions to some of the Arg-gingipains. This study aimed to determine th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01868-07

    authors: Rangarajan M,Aduse-Opoku J,Paramonov N,Hashim A,Bostanci N,Fraser OP,Tarelli E,Curtis MA

    更新日期:2008-04-01 00:00:00

  • Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms.

    abstract::The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.14.4066-4073.2003

    authors: Chou CC,Lin TW,Chen CY,Wang AH

    更新日期:2003-07-01 00:00:00

  • Expression of Vibrio vulnificus capsular polysaccharide inhibits biofilm formation.

    abstract::Vibrio vulnificus is a human pathogen that produces lethal septicemia in susceptible persons, and the primary virulence factor for this organism is capsular polysaccharide (CPS). The role of the capsule in V. vulnificus biofilms was examined under a variety of conditions, by using either defined CPS mutants or spontan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.3.889-893.2004

    authors: Joseph LA,Wright AC

    更新日期:2004-02-01 00:00:00

  • Evidence that TET protein functions as a multimer in the inner membrane of Escherichia coli.

    abstract::The inner membrane TET (TetA) protein, which is involved in Tn10-mediated microbial tetracycline resistance, consists of two domains, alpha and beta, both of which are needed for tetracycline resistance and efflux (M.S. Curiale, L.M. McMurry, and S.B. Levy, J. Bacteriol. 157:211-217, 1984). Since tetracycline-sensitiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1715-1720.1988

    authors: Hickman RK,Levy SB

    更新日期:1988-04-01 00:00:00

  • Effect of the proline analogue baikiain on proline metabolism in Salmonella typhimurium.

    abstract::A proline analogue, 4,5-dehydro-l-pipecolic acid (baikiain) induces the formation in Salmonella typhimurium of the two enzymes catalyzing the degradation of proline, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid (P5C) dehydrogenase. The level of induction by 20 mm baikiain is about 10% of the maximum level ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1134-1141.1972

    authors: Dendinger S,Brill WJ

    更新日期:1972-12-01 00:00:00

  • CHANGES IN MOUSE PNEUMONITIS AGENT ASSOCIATED WITH DEVELOPMENT OF RESISTANCE TO CHLORTETRACYCLINE.

    abstract::Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.17-22.1965

    authors: MOULDER JW,NOVOSEL DL,TRIBBY II

    更新日期:1965-01-01 00:00:00

  • Protein phosphorylation in Bradyrhizobium japonicum bacteroids and cultures.

    abstract::Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.6.3420-3426.1989

    authors: Karr DB,Emerich DW

    更新日期:1989-06-01 00:00:00

  • Cloning and sequencing of a Bacteroides ruminicola B(1)4 endoglucanase gene.

    abstract::Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.3620-3630.1990

    authors: Matsushita O,Russell JB,Wilson DB

    更新日期:1990-07-01 00:00:00

  • Detoxification of protoanemonin by dienelactone hydrolase.

    abstract::Protoanemonin is a toxic metabolite which may be formed during the degradation of some chloroaromatic compounds, such as polychlorinated biphenyls, by natural microbial consortia. We show here that protoanemonin can be transformed by dienelactone hydrolase of Pseudomonas sp. strain B13 to cis-acetylacrylate. Although ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.2.400-402.1998

    authors: Brückmann M,Blasco R,Timmis KN,Pieper DH

    更新日期:1998-01-01 00:00:00

  • Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32).

    abstract::Escherichia coli K-12 produces at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E. coli chromosome. Cloning and examination of novel heat shock pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.14.4247-4253.1991

    authors: Kitagawa M,Wada C,Yoshioka S,Yura T

    更新日期:1991-07-01 00:00:00

  • Modulation of luminescence operon expression by N-octanoyl-L-homoserine lactone in ainS mutants of Vibrio fischeri.

    abstract::Population density-dependent expression of luminescence in Vibrio fischeri is controlled by the autoinducer N-3-oxohexanoyl-L-homoserine lactone (autoinducer 1 [AI-1]), which via LuxR activates transcription of the lux operon (luxICDABEG, encoding the putative autoinducer synthase [LuxI] and the luminescence enzymes)....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.4.971-976.1996

    authors: Kuo A,Callahan SM,Dunlap PV

    更新日期:1996-02-01 00:00:00