Organization of rRNA genes in Mycobacterium bovis BCG.


:The number of rRNA genes in Mycobacterium bovis BCG was examined by Southern hybridization of end-labeled 5S, 16S, and 23S rRNAs with BamHI, PstI, and SalI digests of M. bovis BCG DNA. Each RNA probe gave only one radioactive band with three kinds of DNA digest. These results suggest that M. bovis BCG chromosomes may carry only a minimum set of rRNA genes. Hybridization of randomly labeled rRNAs with BamHI, PstI, SalI, BglII, and PvuII digests of DNA from the same organism supported these conclusions. The 6.4-kilobase-pair SalI fragment containing the entire structural genes for both 16S and 23S rRNAs was cloned into pBR322. The cloned fragment was characterized by restriction endonuclease mapping, DNA-RNA hybridization analysis, and the R-loop technique. The results indicated that the fragments contained rRNA genes in the following order: 16S, 23S, and 5S rRNA genes. No tRNA gene was detected in the spacer region between the 16S and 23S rRNA genes, but one was found downstream of the 23S rRNA and 5S rRNA genes.


J Bacteriol


Journal of bacteriology


Suzuki Y,Yoshinaga K,Ono Y,Nagata A,Yamada T




Has Abstract


1987-02-01 00:00:00












  • Contribution of the periplasmic chaperone Skp to efficient presentation of the autotransporter IcsA on the surface of Shigella flexneri.

    abstract::IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wagner JK,Heindl JE,Gray AN,Jain S,Goldberg MB

    更新日期:2009-02-01 00:00:00

  • Isolation and characterization of a sporulation initiation mutation in the Bacillus subtilis secA gene.

    abstract::A Bacillus subtilis secA mutant, secA12, which is blocked at an early stage of sporulation, is able to grow as well as the wild-type strain at all temperatures tested. Experiments with lacZ fusion genes showed that the induction of kinA expression, as well as the sporulation-specific transcription of the spo0A gene, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Asai K,Kawamura F,Sadaie Y,Takahashi H

    更新日期:1997-01-01 00:00:00

  • Pleiotropic effect of a histidine kinase on carbohydrate metabolism in Synechocystis sp. strain PCC 6803 and its requirement for heterotrophic growth.

    abstract::The deletion of a gene coding for a histidine kinase (sll0750, Hik8) in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 resulted in a conditional lethal phenotype with a pleiotropic effect on the expression of genes involved in glucose metabolism. This mutant had comparable doubling times to wild type...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Singh AK,Sherman LA

    更新日期:2005-04-01 00:00:00

  • Identification of a secreted cholesterol-dependent cytolysin (mitilysin) from Streptococcus mitis.

    abstract::We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

    更新日期:2007-01-01 00:00:00

  • Measuring virulence factor expression by the pathogenic bacterium Photorhabdus luminescens in culture and during insect infection.

    abstract::During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Daborn PJ,Waterfield N,Blight MA,Ffrench-Constant RH

    更新日期:2001-10-01 00:00:00

  • The thiol:disulfide oxidoreductase DsbB mediates the oxidizing effects of the toxic metalloid tellurite (TeO32-) on the plasma membrane redox system of the facultative phototroph Rhodobacter capsulatus.

    abstract::The highly toxic oxyanion tellurite (TeO3(2-)) is a well known pro-oxidant in mammalian and bacterial cells. This work examines the effects of tellurite on the redox state of the electron transport chain of the facultative phototroph Rhodobacter capsulatus, in relation to the role of the thiol:disulfide oxidoreductase...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Borsetti F,Francia F,Turner RJ,Zannoni D

    更新日期:2007-02-01 00:00:00

  • A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis.

    abstract::Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modificat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Taguchi F,Ogawa Y,Takeuchi K,Suzuki T,Toyoda K,Shiraishi T,Ichinose Y

    更新日期:2006-12-01 00:00:00

  • Role of pili in bacterial conjugation.

    abstract::We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and al...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ou JT,Anderson TF

    更新日期:1970-06-01 00:00:00

  • Expression of the cloned uvrB gene of Escherichia coli: dependency on nonsense suppressors.

    abstract::Recombinant plasmid pNP5, consisting of plasmid pMB9 on which the uvrB gene is cloned, fully complements for the defects due to chromosomal uvrB mutations in the presence of the amber suppressor sup-6 or supF. Correndonuclease II activity was also completely restored in in UvrB strains containing both plasmid pNP5 and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pannekoek H,Noordermeer I,van de Putte P

    更新日期:1979-07-01 00:00:00

  • The stability region of the large virulence plasmid of Shigella flexneri encodes an efficient postsegregational killing system.

    abstract::The large virulence plasmid pMYSH6000 of Shigella flexneri contains a determinant that is highly effective in stabilizing otherwise unstable plasmids in Escherichia coli. Expression of two small contiguous genes, mvpA and mvpT (formerly termed STBORF1 and STBORF2), was shown to be sufficient for stability. Mutations i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sayeed S,Reaves L,Radnedge L,Austin S

    更新日期:2000-05-01 00:00:00

  • Characterization of two glyceraldehyde-3-phosphate dehydrogenase isoenzymes from the pentalenolactone producer Streptomyces arenae.

    abstract::Pentalenolactone (PL) irreversibly inactivates the enzyme glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)] (EC and thus is a potent inhibitor of glycolysis in both procaryotic and eucaryotic cells. We showed that PL-producing strain Streptomyces ar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Maurer KH,Pfeiffer F,Zehender H,Mecke D

    更新日期:1983-02-01 00:00:00

  • Outer layers of the Azotobacter vinelandii cyst.

    abstract::Ruthenium red stained a capsule external to the exine of the Azotobacter cyst. The central body is therefore surrounded by three layers, the intine, the exine, and the capsule, all containing acid mucopolysaccharide. Vesicles that appear to originate from the contracting cell membrane of the central body may account f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pope LM,Wyss O

    更新日期:1970-04-01 00:00:00

  • Functional characterization of a replication initiator protein.

    abstract::Functional domains in the RepI replication initiator protein have been identified by classical and site-directed mutagenesis techniques. Mutations conferring an increase in plasmid copy number contained alterations in a key position of a putative helix-turn-helix DNA binding motif. The mutations did not appear to affe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gammie AE,Tolmasky ME,Crosa JH

    更新日期:1993-06-01 00:00:00

  • Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.

    abstract::Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fuangthong M,Helmann JD

    更新日期:2003-11-01 00:00:00

  • A novel hydrolytic dehalogenase for the chlorinated aromatic compound chlorothalonil.

    abstract::Dehalogenases play key roles in the detoxification of halogenated aromatics. Interestingly, only one hydrolytic dehalogenase for halogenated aromatics, 4-chlorobenzoyl-coenzyme A (CoA) dehalogenase, has been reported. Here, we characterize another novel hydrolytic dehalogenase for a halogenated aromatic compound from ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang G,Li R,Li S,Jiang J

    更新日期:2010-06-01 00:00:00

  • Glycerol is metabolized in a complex and strain-dependent manner in Enterococcus faecalis.

    abstract::Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bizzini A,Zhao C,Budin-Verneuil A,Sauvageot N,Giard JC,Auffray Y,Hartke A

    更新日期:2010-02-01 00:00:00

  • An Extended Cyclic Di-GMP Network in the Predatory Bacterium Bdellovibrio bacteriovorus.

    abstract:UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rotem O,Nesper J,Borovok I,Gorovits R,Kolot M,Pasternak Z,Shin I,Glatter T,Pietrokovski S,Jenal U,Jurkevitch E

    更新日期:2015-08-31 00:00:00

  • Isolation of flagella from the archaebacterium Methanococcus voltae by phase separation with Triton X-114.

    abstract::The flagella of Methanococcus voltae were isolated by using three procedures. Initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in KBr gradients. Flagella were also prepared by solubilization of cells with 1% (vol/vol) Triton X-100 and purified as de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kalmokoff ML,Jarrell KF,Koval SF

    更新日期:1988-04-01 00:00:00

  • Interaction of alpha-agglutinin with Saccharomyces cerevisiae a cells.

    abstract::Binding of Saccharomyces cerevisiae alpha-agglutinin to target a cells was assayed by agglutination inhibition and 125I-alpha-agglutinin binding. The assays showed characteristics of equilibrium binding, namely saturability, competability, and the establishment of a kinetic endpoint in the presence of free alpha-agglu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lipke PN,Terrance K,Wu YS

    更新日期:1987-02-01 00:00:00


    abstract::Buck, C. A. (Kansas State University, Manhattan), R. L. Anacker, F. S. Newman, and A. Eisenstark. Phage isolated from lysogenic Bacillus anthracis. J. Bacteriol. 85:1423-1430. 1963.-Bacillus anthracis cultures (a total of 311) were obtained from several laboratories throughout the world; 139 of these were screened for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1963-06-01 00:00:00

  • Initiation of swarming motility by Proteus mirabilis occurs in response to specific cues present in urine and requires excess L-glutamine.

    abstract::Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Armbruster CE,Hodges SA,Mobley HL

    更新日期:2013-03-01 00:00:00

  • Isolation and characterization of an Escherichia coli mutant deficient in dTMP kinase activity.

    abstract::Escherichia coli LD0181 is sensitive to 15 micrograms of 2',3'-dideoxythymidine per ml. A derivative that was resistant to 40 micrograms of the same chemical per ml at 30 degrees C and that had lost the ability to grow on enriched medium at 42 degrees C was isolated after nitroso-guanidine mutagenesis. This mutant, TD...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Daws TD,Fuchs JA

    更新日期:1984-02-01 00:00:00

  • Mycoplasma laidlawii in human burns.

    abstract::Mycoplasma laidlawii was recovered from burns from 13 of 52 patients. In most cases, cocci or bacilli were isolated simultaneously. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Markham JG,Markham NP

    更新日期:1969-05-01 00:00:00

  • Neurospora crassa mutants deficient in asparagine synthetase.

    abstract::Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: MacPhee KG,Nelson RE,Schuster SM

    更新日期:1983-10-01 00:00:00

  • Dynamics of the SetCD-regulated integration and excision of genomic islands mobilized by integrating conjugative elements of the SXT/R391 family.

    abstract::Mobilizable genomic islands (MGIs) are small genomic islands that are mobilizable by SXT/R391 integrating conjugative elements (ICEs) due to similar origins of transfer. Their site-specific integration and excision are catalyzed by the integrase that they encode, but their conjugative transfer entirely depends upon th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Daccord A,Mursell M,Poulin-Laprade D,Burrus V

    更新日期:2012-11-01 00:00:00

  • Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi.

    abstract::A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kotoujansky A,Lemattre M,Boistard P

    更新日期:1982-04-01 00:00:00

  • Stability of ribosomes and ribosomal ribonucleic acid from Bacillus stearothermophilus.

    abstract::After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Friedman SM,Axel R,Weinstein IB

    更新日期:1967-05-01 00:00:00

  • Spontaneous erythromycin resistance mutation in a 23S rRNA gene, rrlA, of the extreme thermophile Thermus thermophilus IB-21.

    abstract::Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gregory ST,Cate JH,Dahlberg AE

    更新日期:2001-07-01 00:00:00

  • AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor.

    abstract::In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and Bacillus subtilisStreptomyces is a bacterial genus that is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Łebkowski T,Wolański M,Ołdziej S,Flärdh K,Zakrzewska-Czerwińska J

    更新日期:2020-01-15 00:00:00


    abstract::Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-11-01 00:00:00