Organization of rRNA genes in Mycobacterium bovis BCG.


:The number of rRNA genes in Mycobacterium bovis BCG was examined by Southern hybridization of end-labeled 5S, 16S, and 23S rRNAs with BamHI, PstI, and SalI digests of M. bovis BCG DNA. Each RNA probe gave only one radioactive band with three kinds of DNA digest. These results suggest that M. bovis BCG chromosomes may carry only a minimum set of rRNA genes. Hybridization of randomly labeled rRNAs with BamHI, PstI, SalI, BglII, and PvuII digests of DNA from the same organism supported these conclusions. The 6.4-kilobase-pair SalI fragment containing the entire structural genes for both 16S and 23S rRNAs was cloned into pBR322. The cloned fragment was characterized by restriction endonuclease mapping, DNA-RNA hybridization analysis, and the R-loop technique. The results indicated that the fragments contained rRNA genes in the following order: 16S, 23S, and 5S rRNA genes. No tRNA gene was detected in the spacer region between the 16S and 23S rRNA genes, but one was found downstream of the 23S rRNA and 5S rRNA genes.


J Bacteriol


Journal of bacteriology


Suzuki Y,Yoshinaga K,Ono Y,Nagata A,Yamada T




Has Abstract


1987-02-01 00:00:00












  • Klebsiella serotype 25 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase.

    abstract::By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Niemann H,Kwiatkowski B,Westphal U,Stirm S

    更新日期:1977-04-01 00:00:00

  • Infection in mouse peritoneal cavity with a pyrimidine-requiring mutant and naturally occurring Staphylococcus aureus strains.

    abstract::The lethal activity of a thymineless mutant of Staphylococcus aureus Wood 46 strain has been compared with that of three naturally occurring strains: parent Wood 46, Smith, and coagulase-negative SA-13. The thymineless mutant and the parent Wood 46 strain showed a sharp decline in culturable units from the peritoneal ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mathieu LG,De Repentigny J,Turgeon S,Sonea S

    更新日期:1967-07-01 00:00:00

  • Induction of yeast killer factor mutations.

    abstract::Two related killer strains of Saccharomyces cerevisiae were mutagenized and screened for nonkiller variants. About 20% of the mutants derived from one strain lacked all detectable double-straned ribonucleic acid (dsRNA). About 70% of the mutants from the other strain lacked one of the dsRNA species normally associated...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vodkin M

    更新日期:1977-10-01 00:00:00

  • Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis.

    abstract::Protoplasts of Saccharomyces cerevisiae were mixed with linear DNA plasmids, pGKl1 and pGKl2, isolated from a Kluyveromyces lactis killer strain and treated with polyethylene glycol. Out of 2,000 colonies regenerated on a nonselective medium, two killer transformants were obtained. The pGKl plasmids and the killer cha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gunge N,Murata K,Sakaguchi K

    更新日期:1982-07-01 00:00:00

  • Pathway of succinate and propionate formation in Bacteroides fragilis.

    abstract::Cell suspensions of Bacteroides fragilis were allowed to ferment glucose and lactate labeled with (14)C in different positions. The fermentation products, propionate and acetate, were isolated, and the distribution of radioactivity was determined. An analysis of key enzymes of possible pathways was also made. The resu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Macy JM,Ljungdahl LG,Gottschalk G

    更新日期:1978-04-01 00:00:00

  • Oligoribonuclease is distinct from the other known exoribonucleases of Escherichia coli.

    abstract::Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yu D,Deutscher MP

    更新日期:1995-07-01 00:00:00

  • Polyamine transport and role of potE in response to osmotic stress in Escherichia coli.

    abstract::When transport of polyamines in Escherichia coli was examined, putrescine excretion was observed under two different physiological conditions: (i) strictly correlated to growth and (ii) following a hyperosmotic shock. Spermidine was not excreted. Characterization of a deletion mutant showed that PotE is not involved i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schiller D,Kruse D,Kneifel H,Krämer R,Burkovski A

    更新日期:2000-11-01 00:00:00

  • Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli.

    abstract::Type IIb heat-labile enterotoxin (LT-IIb) is produced by Escherichia coli 41. Restriction fragments of total cell DNA from strain 41 were cloned into a cosmid vector, and one cosmid clone that encoded LT-IIb was identified. The genes for LT-IIb were subcloned into a variety of plasmids, expressed in minicells, sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pickett CL,Twiddy EM,Coker C,Holmes RK

    更新日期:1989-09-01 00:00:00

  • VirE1 protein mediates export of the single-stranded DNA-binding protein VirE2 from Agrobacterium tumefaciens into plant cells.

    abstract::Agrobacterium tumefaciens transfers single-stranded DNAs (T strands) into plant cells. VirE1 and VirE2, which is a single-stranded DNA binding protein, are important for tumorigenesis. We show that T strands and VirE2 can enter plant cells independently and that export of VirE2, but not of T strands, depends on VirE1....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sundberg C,Meek L,Carroll K,Das A,Ream W

    更新日期:1996-02-01 00:00:00

  • Halocin S8: a 36-amino-acid microhalocin from the haloarchaeal strain S8a.

    abstract::Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Price LB,Shand RF

    更新日期:2000-09-01 00:00:00

  • Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D.

    abstract::The gene (entD) encoding staphylococcal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bayles KW,Iandolo JJ

    更新日期:1989-09-01 00:00:00

  • Orientation of the guanine operon of Escherichia coli K-12 by utilizing strains containing guaB-xse and guaB-upp deletions.

    abstract::Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vales LD,Chase JW,Murphy JB

    更新日期:1979-07-01 00:00:00

  • Bacteriocin activity of Streptococcus pneumoniae is controlled by the serine protease HtrA via posttranscriptional regulation.

    abstract::The blp locus of a type 6A strain of Streptococcus pneumoniae encodes a two-peptide bacteriocin, pneumocin MN, which mediates intraspecies competition during mouse nasopharyngeal colonization. This locus is regulated by a quorum-sensing mechanism consisting of a dedicated two-component regulatory system and a peptide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dawid S,Sebert ME,Weiser JN

    更新日期:2009-03-01 00:00:00

  • Relationship between sulfadiazine resistance and the failure to ferment maltose in Neisseria meningitidis.

    abstract::The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kingsbury DT

    更新日期:1967-09-01 00:00:00

  • Resistance of Escherichia coli to penicillins. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains.

    abstract::The chromosomally mediated penicillinase present in three strains of Escherichia coli K-12 has been purified and characterized. Two of the strains carried the ampA gene and the third the wild-type allele. The purification involves release of the enzyme by spheroplast formation, dialysis, chromatography on sulfoethyl c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Linström EB,Boman HG,Steele BB

    更新日期:1970-01-01 00:00:00

  • Impact of phosphorylation of specific residues in the tyrosine autokinase, Wzc, on its activity in assembly of group 1 capsules in Escherichia coli.

    abstract::Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Paiment A,Hocking J,Whitfield C

    更新日期:2002-12-01 00:00:00

  • In vivo-selected mutations in methyl-directed mismatch repair suppress the virulence attenuation of Salmonella dam mutant strains following intraperitoneal, but not oral, infection of naïve mice.

    abstract::Salmonella enterica serovar Typhimurium that lacks the DNA adenine methylase (Dam) ectopically expresses multiple genes that are preferentially expressed during infection, is attenuated for virulence, and confers heightened immunity in vaccinated hosts. The safety of dam mutant Salmonella vaccines was evaluated by scr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Heithoff DM,Badie G,Julio SM,Enioutina EY,Daynes RA,Sinsheimer RL,Mahan MJ

    更新日期:2007-07-01 00:00:00

  • Shuttle plasmids for Escherichia coli and Clostridium perfringens.

    abstract::Small plasmids which replicate in both Escherichia coli and Clostridium perfringens were made by recombining E. coli plasmid pBR322 with three different small (less than 4 kilobases) plasmids native to C. perfringens. Subsequently, two homologous, though distinct, tetracycline resistance determinants (tet) from other ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Squires CH,Heefner DL,Evans RJ,Kopp BJ,Yarus MJ

    更新日期:1984-08-01 00:00:00

  • Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii.

    abstract::The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Adams MD,Goglin K,Molyneaux N,Hujer KM,Lavender H,Jamison JJ,MacDonald IJ,Martin KM,Russo T,Campagnari AA,Hujer AM,Bonomo RA,Gill SR

    更新日期:2008-12-01 00:00:00

  • Selection of araB and araC mutants of Escherichia coli B-r by resistance to ribitol.

    abstract::The growth of strain araC(c)67, which produces the enzymes of the ara operon constitutively, is inhibited by the addition of ribitol. Isolation of strains resistant to ribitol yields mutants of either the araB or araC genes. A model to account for the inhibition by ribitol is discussed. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Katz L

    更新日期:1970-05-01 00:00:00

  • Biochemical and phylogenetic characterization of a novel diaminopimelate biosynthesis pathway in prokaryotes identifies a diverged form of LL-diaminopimelate aminotransferase.

    abstract::A variant of the diaminopimelate (DAP)-lysine biosynthesis pathway uses an LL-DAP aminotransferase (DapL, EC to catalyze the direct conversion of L-2,3,4,5-tetrahydrodipicolinate to LL-DAP. Comparative genomic analysis and experimental verification of DapL candidates revealed the existence of two diverged fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hudson AO,Gilvarg C,Leustek T

    更新日期:2008-05-01 00:00:00

  • Biosynthetic pathways of the osmolytes N epsilon-acetyl-beta-lysine, beta-glutamine, and betaine in Methanohalophilus strain FDF1 suggested by nuclear magnetic resonance analyses.

    abstract::Methanohalophilus strain FDF1 synthesizes beta-glutamine, betaine, and N epsilon-acetyl-beta-lysine as osmoprotective agents when the cells are grown in high external concentrations of NaCl. Nuclear magnetic resonance spectroscopic analyses of 13CH3OH-12CO2 label incorporation by the cells provide information on the b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Roberts MF,Lai MC,Gunsalus RP

    更新日期:1992-10-01 00:00:00

  • Analysis of the roles of FlgP and FlgQ in flagellar motility of Campylobacter jejuni.

    abstract::Flagellar motility is an important determinant of Campylobacter jejuni that is required for promoting interactions with various hosts to promote gastroenteritis in humans or commensal colonization of many animals. In a previous study, we identified a nonmotile mutant of C. jejuni 81-176 with a transposon insertion in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sommerlad SM,Hendrixson DR

    更新日期:2007-01-01 00:00:00

  • Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei.

    abstract::Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Birnbaum J,Lichstein HC

    更新日期:1966-10-01 00:00:00

  • Regulation of the aromatic pathway in the cyanobacterium Synechococcus sp. strain Pcc6301 (Anacystis nidulans).

    abstract::A pattern of allosteric control for aromatic biosynthesis in cyanobacteria relies upon early-pathway regulation as the major control point for the entire branched pathway. In Synechococcus sp. strain PCC6301 (Anacystis nidulans), two enzymes which form precursors for L-phenylalanine biosynthesis are subject to control...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hall GC,Flick MB,Jensen RA

    更新日期:1983-01-01 00:00:00

  • Regulatory role of recF in the SOS response of Escherichia coli: impaired induction of SOS genes by UV irradiation and nalidixic acid in a recF mutant.

    abstract::We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Thoms B,Wackernagel W

    更新日期:1987-04-01 00:00:00

  • Growth and glucose repression are controlled by glucose transport in Saccharomyces cerevisiae cells containing only one glucose transporter.

    abstract::A set of Saccharomyces cerevisiae strains with variable expression of only the high-affinity Hxt7 glucose transporter was constructed by partial deletion of the HXT7 promoter in vitro and integration of the gene at various copy numbers into the genome of an hxt1-7 gal2 deletion strain. The glucose transport capacity i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ye L,Kruckeberg AL,Berden JA,van Dam K

    更新日期:1999-08-01 00:00:00

  • Differential inactivation of alcohol dehydrogenase isoenzymes in Zymomonas mobilis by oxygen.

    abstract::Zymomonas mobilis is endowed with two isoenzymes of fermentative alcohol dehydrogenase, a zinc-containing enzyme (ADH I) and an iron-containing enzyme (ADH II). The activity of ADH I remains fully conserved, while ADH II activity decays when anaerobic cultures are shifted to aerobiosis. This differential response depe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tamarit J,Cabiscol E,Aguilar J,Ros J

    更新日期:1997-02-01 00:00:00

  • Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans.

    abstract::The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Garrad RC,Bhattacharjee JK

    更新日期:1992-11-01 00:00:00

  • Complete genome sequence of Streptococcus equi subsp. zooepidemicus strain ATCC 35246.

    abstract::Streptococcus equi subsp. zooepidemicus is an opportunistic pathogen. It has caused a very large economic loss in the swine industry of China and has become a threat to human health. We announce the complete genome sequence of S. equi subsp. zooepidemicus strain ATCC 35246, which provides opportunities to understand i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ma Z,Geng J,Zhang H,Yu H,Yi L,Lei M,Lu CP,Fan HJ,Hu S

    更新日期:2011-10-01 00:00:00