Abstract:
:A previously constructed trp-S-lacZ fusion encoding a hybrid protein with beta-galactosidase activity was subcloned from a multicopy plasmid onto a lambda vector. Single-copy lysogens of lambda trpS-lacZ were used to determine whether trpS was regulated in a manner similar to that of other aminoacyl-tRNA synthetases. trpS regulation was found to resemble that of the majority of synthetases, in that expression of the lysogen-encoded hybrid beta-galactosidase varied with growth rate; beta-galactosidase activity increased 2.5-fold as the generation time decreased from 150 to 37 min. This regulatory response was confirmed by DNA/RNA hybridization experiments, which also suggested that this form of metabolic regulation occurred at the transcriptional level. No alteration in the level of hybrid beta-galactosidase was observed, however, when cells were starved for tryptophan.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hall CV,Yanofsky Cdoi
10.1128/JB.151.2.918-923.1982subject
Has Abstractpub_date
1982-08-01 00:00:00pages
918-23issue
2eissn
0021-9193issn
1098-5530journal_volume
151pub_type
杂志文章abstract::Bacterial uptake of phosphate is usually accomplished via high-affinity transporters that are commonly regulated by two-component systems, which are activated when the concentration of phosphate is low. Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transpo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01965-14
更新日期:2014-10-01 00:00:00
abstract::MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We al...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00371-10
更新日期:2010-09-01 00:00:00
abstract::An attempt was made to explain the puzzling observation that in bacteria 2,6-diaminopurine can replace guanine for guanineless mutants and for xanthineless mutants (both of which can make adenosine monophosphate de novo) but not for nonexacting purine auxotrophs (which cannot make adenosine monophosphate de novo). The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.565-571.1968
更新日期:1968-02-01 00:00:00
abstract::Sequences of the major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omcB) from Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. The Sawyer runs test, compatibility matrices...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.5997-6008.2001
更新日期:2001-10-01 00:00:00
abstract::Replication of the broad-host-range, IncPalpha plasmid RK2 requires two plasmid loci: trfA, the replication initiator gene, and oriV, the origin of replication. While these determinants are sufficient for replication in a wide variety of bacteria, they do not confer the stable maintenance of parental RK2 observed in i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.21.6014-6026.2000
更新日期:2000-11-01 00:00:00
abstract::Goldman, Manuel (The University of Michigan, Ann Arbor), and Harold J. Blumenthal. Pathways of glucose catabolism in Bacillus cereus. J. Bacteriol. 87:377-386. 1964.-Estimates by a radiorespirometric method of the pathways of glucose catabolism of resting-cell suspensions of Bacillus cereus strain terminalis indicate ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.2.377-386.1964
更新日期:1964-02-01 00:00:00
abstract::Biodegradation of metal-citrate complexes by Pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. Bidentate Fe(III)-, Ni-, and Zn-citrate complexes were readily biodegraded, but the tridentate Cd- and Cu-citrate, and U-citrate complexes were not. The biodegradation of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.8.1989-1993.1995
更新日期:1995-04-01 00:00:00
abstract::Mutant strains of Aspergillus niger with reduced citrate control of carbohydrate catabolism (cic mutants) grow faster than the parent strain on media containing 5% (wt/vol) citrate. The mutants tolerated a higher intracellular citrate concentration than the parent strain. One mutant (cic-7/3) contained phosphofructoki...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.1019-1022.1986
更新日期:1986-03-01 00:00:00
abstract::In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00799-16
更新日期:2017-05-09 00:00:00
abstract::Chemical, physical, and immunological properties of the envelope antigen of Yersinia pestis strains have been investigated. The antigen consists of two components with isoelectric points (pI) of 4.6 and 4.8. One component (pI 4.6) is a protein bound to a small carbohydrate moiety identified as an oligomeric galactan; ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.1.48-55.1974
更新日期:1974-01-01 00:00:00
abstract::The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00213-10
更新日期:2010-06-01 00:00:00
abstract::The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01520-06
更新日期:2007-03-01 00:00:00
abstract::We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and al...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.3.648-654.1970
更新日期:1970-06-01 00:00:00
abstract::Phase-contrast and serial-section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1430-1438.1986
更新日期:1986-12-01 00:00:00
abstract::The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01690-09
更新日期:2010-05-01 00:00:00
abstract::The DnaA protein concentration in Escherichia coli was increased above the wild-type level by inducing a lacP-controlled dnaA gene located on a plasmid. In these cells with different DnaA protein levels, we measured several parameters: dnaA gene expression; cell size, amount of DNA per cell, and number of origins per ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.20.6537-6545.1993
更新日期:1993-10-01 00:00:00
abstract::Campylobacter jejuni, a microaerophilic bacterium, is the most frequent cause of human bacterial gastroenteritis. C. jejuni is exposed to harmful reactive oxygen species (ROS) produced during its own normal metabolic processes and during infection from the host immune system and from host intestinal microbiota. These ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05740-11
更新日期:2012-01-01 00:00:00
abstract::Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.344-353.1975
更新日期:1975-01-01 00:00:00
abstract::The Sinorhizobium meliloti megaplasmid pSymA has previously been implicated in gluconate utilization. We report a locus on pSymA encoding a putative tripartite ATP-independent periplasmic (TRAP) transporter that is required for gluconate utilization. The expression of this locus is negatively regulated by a GntR famil...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01317-08
更新日期:2009-02-01 00:00:00
abstract::The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2385-2393.1999
更新日期:1999-04-01 00:00:00
abstract::Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01412-08
更新日期:2009-04-01 00:00:00
abstract::Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5999-6006.1994
更新日期:1994-10-01 00:00:00
abstract::The ubiquitous opportunistic pathogen Pseudomonas aeruginosa has five aerobic terminal oxidases: bo(3)-type quinol oxidase (Cyo), cyanide-insensitive oxidase (CIO), aa3-type cytochrome c oxidase (aa3), and two cbb(3)-type cytochrome c oxidases (cbb(3)-1and cbb(3)-2). These terminal oxidases are differentially regulate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02176-14
更新日期:2014-12-01 00:00:00
abstract::Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.), William Shirley, and John S. Baumstark. Effect of osmolarity on phagocytosis. J. Bacteriol. 85:306-313. 1963.-The effect of a number of different compounds on phagocytosis was studied. Phagocytosis was monitored by morphological and biochemical means. It was...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.2.306-313.1963
更新日期:1963-02-01 00:00:00
abstract::The fis operon from Salmonella typhimurium has been cloned and sequenced, and the properties of Fis-deficient and Fis-constitutive strains were examined. The overall fis operon organization in S. typhimurium is the same as that in Escherichia coli, with the deduced Fis amino acid sequences being identical between both...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.8.2021-2032.1995
更新日期:1995-04-01 00:00:00
abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00504-20
更新日期:2020-11-04 00:00:00
abstract::We studied the activity of a debranching enzyme (TreX) from Sulfolobus solfataricus on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc₄-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01442-13
更新日期:2014-06-01 00:00:00
abstract::To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.16.5286-5288.1993
更新日期:1993-08-01 00:00:00
abstract::Pseudomonas syringae injects numerous bacterial proteins into host plant cells through a type 3 secretion system (T3SS). One of the first such bacterial effectors discovered, HopA1, is a protein that has unknown functions in the host cell but possesses close homologs that trigger the plant hypersensitive response in r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01621-12
更新日期:2013-02-01 00:00:00
abstract::Salmonella enterica can utilize fructose-asparagine (F-Asn) as a source of carbon and nitrogen. This capability has been attributed to five genes in the fra locus. Previously, we determined that mutations in fraB (deglycase), fraD (kinase), or fraA (transporter) eliminated the ability of Salmonella to grow on F-Asn, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00330-17
更新日期:2017-10-17 00:00:00