Role of pili in bacterial conjugation.

Abstract:

:We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and allowed to grow into clones. That many exconjugants derived from "loose"-mating pairs produced recombinants suggests that F pili are involved in the transfer of genetic material. The frequency of formation of recombinants from "close"-mating pairs, however, was significantly higher than that from loose-mating pairs, indicating that a close cell-to-cell contact facilitates chromosome transfer. Death rates of exconjugants from close pairs were also higher than those from loose pairs. Hfr x F(-) matings produced higher death rates than F(+) x F(-) matings. Male cells were found capable of transferring genetic markers to two F(-) cells simultaneously. We conclude that F pili play at least three roles in mating: (i) they initiate contacts between mating pairs; (ii) they facilitate the transfer of genetic material; and (iii) they draw mating cells into a close contact which increases the fertility of the union.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ou JT,Anderson TF

doi

10.1128/JB.102.3.648-654.1970

subject

Has Abstract

pub_date

1970-06-01 00:00:00

pages

648-54

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

102

pub_type

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