Inhibition of secretion of a mutant lipoprotein across the cytoplasmic membrane by the wild-type lipoprotein of the Escherichia coli outer membrane.

Abstract:

:A globomycin-resistant mutant of Escherichia coli was found to produce a precursor of the major outer membrane lipoprotein (prolipoprotein), in which the glycine residue at position 14 within the signal peptide was replaced by an aspartic acid residue. The same mutation has been reported by Lin et al. (Proc. Natl. Acad. Sci. U.S.A. 175:4891-4895, 1978). The structural gene of the mutant prolipoprotein was inserted into an inducible expression cloning vehicle. When the mutant prolipoprotein was produced in lipoprotein-minus host cells, 82% of the unprocessed protein was found in the membrane fraction, with the remaining 18% localized in the soluble fraction. However, when the production of the mutant prolipoprotein was induced in the wild-type lpp+ host cells, only 31% of the mutant prolipoprotein was found in the membrane fraction, leaving the remaining 69% in the soluble, cytoplasmic fraction. In addition, the assembly of the wild-type lipoprotein in these cells was not affected, whether the mutant prolipoprotein was produced or not. These results suggest that secretions of both mutant and wild-type prolipoproteins utilize the same component(s) responsible for the initial stages of secretion across the cytoplasmic membrane. However, it appears that the wild-type lipoprotein has a higher affinity for these components than does the mutant lipoprotein.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Lee N,Yamagata H,Inouye M

doi

10.1128/JB.155.1.407-411.1983

subject

Has Abstract

pub_date

1983-07-01 00:00:00

pages

407-11

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

155

pub_type

杂志文章
  • Unraveling the function of the Rhodospirillum rubrum activator of polyhydroxybutyrate (PHB) degradation: the activator is a PHB-granule-bound protein (phasin).

    abstract::Efficient hydrolysis of native poly(3-hydroxybutyrate) (nPHB) granules in vitro by soluble PHB depolymerase of Rhodospirillum rubrum requires pretreatment of nPHB with an activator compound present in R. rubrum cells (J. M. Merrick and M. Doudoroff, J. Bacteriol. 88:60-71, 1964). Edman sequencing of the purified activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.8.2466-2475.2004

    authors: Handrick R,Reinhardt S,Schultheiss D,Reichart T,Schüler D,Jendrossek V,Jendrossek D

    更新日期:2004-04-01 00:00:00

  • Cadmium and manganese transport in Staphylococcus aureus membrane vesicles.

    abstract::The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.2.973-976.1982

    authors: Perry RD,Silver S

    更新日期:1982-05-01 00:00:00

  • Cell wall constituents of Leuconostoc citrovorum and Leuconostoc mesenteroides.

    abstract::The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.273-277.1967

    authors: Harney SJ,Simopoulos ND,Ikawa M

    更新日期:1967-01-01 00:00:00

  • Isolation and characterization of trans-acting mutations involved in oxygen regulation of puc operon transcription in Rhodobacter sphaeroides.

    abstract::Transcriptional expression of the puc operon in Rhodobacter sphaeroides 2.4.1 is dependent on the partial pressure of oxygen. By using transcriptional fusions in trans of a promoterless fragment derived from the aminoglycoside-3'-phosphotransferase gene of Tn903 to puc operon-specific DNA containing a 629-bp 5' cis-ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.4.1158-1171.1992

    authors: Lee JK,Kaplan S

    更新日期:1992-02-01 00:00:00

  • Metal ion content of Escherichia coli versus cell age.

    abstract::The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.3.1089-1095.1976

    authors: Kung FC,Raymond J,Glaser DA

    更新日期:1976-06-01 00:00:00

  • Regulation of the cadA cadmium resistance determinant of Staphylococcus aureus plasmid pI258.

    abstract::Regulation of the cadA cadmium and zinc resistance determinant of Staphylococcus aureus plasmid pI258 was demonstrated by using gene fusions and direct measurements of transcription. In growth experiments, cells harboring the intact cadA operon were induced with different cations and challenged by an inhibitory concen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7643-7649.1991

    authors: Yoon KP,Misra TK,Silver S

    更新日期:1991-12-01 00:00:00

  • FK-506-binding proteins from streptomycetes producing immunosuppressive macrolactones of the FK-506 type.

    abstract::FK-506-binding proteins (FKBPs), which in T cells are supposed to mediate the immunosuppressive effects of the compounds FK-506 and rapamycin, have been isolated from Streptomyces chrysomallus, S. hygroscopicus subsp. ascomyceticus, and S. hygroscopicus. The latter two strains are producers of ascomycin (the ethyl ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.18.5888-5894.1992

    authors: Pahl A,Keller U

    更新日期:1992-09-01 00:00:00

  • Functional complementation of the essential gene fabG1 of Mycobacterium tuberculosis by Mycobacterium smegmatis fabG but not Escherichia coli fabG.

    abstract::Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01740-06

    authors: Parish T,Roberts G,Laval F,Schaeffer M,Daffé M,Duncan K

    更新日期:2007-05-01 00:00:00

  • Identification of a segment of the Escherichia coli Tsx protein that functions as a bacteriophage receptor area.

    abstract::The Escherichia coli outer membrane protein Tsx functions as a nucleoside-specific channel and serves as the receptor for colicin K and a number of T-even-type bacteriophages, including phage T6. To identify those segments of the Tsx protein that are important for its phage receptor function, we devised a selection an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.2809-2817.1993

    authors: Schneider H,Fsihi H,Kottwitz B,Mygind B,Bremer E

    更新日期:1993-05-01 00:00:00

  • Nucleotide sequence of the Salmonella typhimurium mutB gene, the homolog of Escherichia coli mutY.

    abstract::The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.2.541-543.1993

    authors: Desiraju V,Shanabruch WG,Lu AL

    更新日期:1993-01-01 00:00:00

  • Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase.

    abstract::Restriction fragments containing the gene encoding cyanase, cynS, without its transcriptional regulatory sequences were placed downstream of lac and tac promoters in various pUC derivatives to maximize production of cyanase. Plasmid pSJ105, which contains the cynS gene and an upstream open reading frame, gave the high...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.5224-5230.1987

    authors: Sung YC,Anderson PM,Fuchs JA

    更新日期:1987-11-01 00:00:00

  • Selection, expression, and nucleotide sequencing of the glutamate dehydrogenase gene of Peptostreptococcus asaccharolyticus.

    abstract::The gene for the catabolic NAD-linked glutamate dehydrogenase of Peptostreptococcus asaccharolyticus was cloned by selection of Escherichia coli for complementation of a biosynthetic defect. Cloned fragments containing the gene and the P. asaccharolyticus transcription and translation signals are very highly expressed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6162-6167.1991

    authors: Snedecor B,Chu H,Chen E

    更新日期:1991-10-01 00:00:00

  • Role and regulation of fatty acid biosynthesis in the response of Shewanella piezotolerans WP3 to different temperatures and pressures.

    abstract::Members of the genus Shewanella inhabit various environments; they are capable of synthesizing various types of low-melting-point fatty acids, including monounsaturated fatty acids (MUFA) and branched-chain fatty acids (BCFA) with and without eicosapentanoic acid (EPA). The genes involved in fatty acid synthesis in 15...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00498-08

    authors: Wang F,Xiao X,Ou HY,Gai Y,Wang F

    更新日期:2009-04-01 00:00:00

  • Regulatory mutants and control of cysteine biosynthetic enzymes in Salmonella typhimurium.

    abstract::The cysB region in Salmonella typhimurium regulates in a positive manner the noncontiguous structural genes for the enzymes responsible for sulfate reduction in cysteine biosynthesis. We treated three cysB mutants with chemical mutagens and selected 81 secondary mutants in which the inability to utilize sulfate was su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.94-101.1976

    authors: Borum PR,Monty KJ

    更新日期:1976-01-01 00:00:00

  • Isocitrate lyase from a thermophilic Bacillus: effect of salts on enzyme activity.

    abstract::The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1160-1169.1973

    authors: Griffiths MW,Sundaram TK

    更新日期:1973-12-01 00:00:00

  • Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape.

    abstract::Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells at 30 degrees C but grew as spherical cells at 42 degrees C. The phenotype of one of these rod(Ts) mutants was 88% cotran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.3.1143-1158.1978

    authors: Iwaya M,Goldman R,Tipper DJ,Feingold B,Strominger JL

    更新日期:1978-12-01 00:00:00

  • Localization of P42 and F(1)-ATPase α-subunit homolog of the gliding machinery in Mycoplasma mobile revealed by newly developed gene manipulation and fluorescent protein tagging.

    abstract::Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01418-13

    authors: Tulum I,Yabe M,Uenoyama A,Miyata M

    更新日期:2014-05-01 00:00:00

  • Recruitment of the ParG segregation protein to different affinity DNA sites.

    abstract::The segrosome is the nucleoprotein complex that mediates accurate plasmid segregation. In addition to its multifunctional role in segrosome assembly, the ParG protein of multiresistance plasmid TP228 is a transcriptional repressor of the parFG partition genes. ParG is a homodimeric DNA binding protein, with C-terminal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01630-08

    authors: Zampini M,Derome A,Bailey SE,Barillà D,Hayes F

    更新日期:2009-06-01 00:00:00

  • Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes.

    abstract::A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.16.4794-4800.1996

    authors: Morbidoni HR,de Mendoza D,Cronan JE Jr

    更新日期:1996-08-01 00:00:00

  • Phenol: a complex chemoeffector in bacterial chemotaxis.

    abstract::Earlier observations that phenol is a repellent for Salmonella typhimurium but an attractant for Escherichia coli were confirmed. This behavioral difference was found to correlate with a difference in the effect phenol had on receptor methylation levels; it caused net demethylation in S. typhimurium but net methylatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.371-379.1987

    authors: Imae Y,Oosawa K,Mizuno T,Kihara M,Macnab RM

    更新日期:1987-01-01 00:00:00

  • Whole-genome sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H.

    abstract::We present the genomic sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H (ATCC 43290), a clinical isolate from the Colorado Department of Health, Denver, CO. This is the first example of a genome sequence of L. pneumophila from a serogroup other than serogroup 1. We highlight the simil...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06626-11

    authors: Amaro F,Gilbert JA,Owens S,Trimble W,Shuman HA

    更新日期:2012-03-01 00:00:00

  • YkdA and YvtA, HtrA-like serine proteases in Bacillus subtilis, engage in negative autoregulation and reciprocal cross-regulation of ykdA and yvtA gene expression.

    abstract::HtrA-type serine proteases participate in folding and degradation of aberrant proteins and in processing and maturation of native proteins. Mutation of the corresponding genes often confers a pleiotropic phenotype that can include temperature sensitivity, sensitivity to osmotic and oxidative stress, and attenuated vir...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.2.654-663.2001

    authors: Noone D,Howell A,Collery R,Devine KM

    更新日期:2001-01-01 00:00:00

  • DNA supercoiling and suppression of the leu-500 promoter mutation.

    abstract::top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.947-949.1985

    authors: Pruss GJ,Drlica K

    更新日期:1985-11-01 00:00:00

  • D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of als regulon expression by allose and ribose.

    abstract::Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Tra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.22.7126-7130.1999

    authors: Poulsen TS,Chang YY,Hove-Jensen B

    更新日期:1999-11-01 00:00:00

  • Gene structures and regulation of the alkane hydroxylase complex in Acinetobacter sp. strain M-1.

    abstract::In the long-chain n-alkane degrader Acinetobacter sp. strain M-1, two alkane hydroxylase complexes are switched by controlling the expression of two n-alkane hydroxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubredoxin ruductase are encoded by a single gene and expressed con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1819-1823.2001

    authors: Tani A,Ishige T,Sakai Y,Kato N

    更新日期:2001-03-01 00:00:00

  • Function of a chemotaxis-like signal transduction pathway in modulating motility, cell clumping, and cell length in the alphaproteobacterium Azospirillum brasilense.

    abstract::A chemotaxis signal transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this pathway are defective in chemotaxis, a mutant in which the entire...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00734-08

    authors: Bible AN,Stephens BB,Ortega DR,Xie Z,Alexandre G

    更新日期:2008-10-01 00:00:00

  • FATTY ACID METABOLISM IN SERRATIA MARCESCENS I. : Oxidation of Saturated Fatty Acids by Whole Cells.

    abstract::Bishop, D. G. (University of Sydney, Sydney, Australia), and J. L. Still. Fatty acid metabolism in Serratia marcescens. J. Bacteriol. 82:370-375. 1961.-A study has been made of the oxidation of saturated fatty acids containing between two and 18 carbon atoms by whole cells of the bacterium, Serratia marcescens. This o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.3.370-375.1961

    authors: Bishop DG,Still JL

    更新日期:1961-09-01 00:00:00

  • Recombination-deficient mutants of colicinogenic Salmonella typhimurium detected by their failure to produce colicin.

    abstract::Survivors of nitrosoguanidine-treated cultures of a colicinogenic strain of Salmonella typhimurium were tested for spontaneous production of colicin E1. Of about 1,000 colonies tested, 13 produced no (or very narrow) colicin zones. Four of these isolates proved to be more sensitive to ultraviolet (UV) light, X rays, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.345-350.1970

    authors: MacPhee DG

    更新日期:1970-10-01 00:00:00

  • Carotenoid-containing outer membrane of Synechocystis sp. strain PCC6714.

    abstract::Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.384-389.1985

    authors: Jürgens UJ,Weckesser J

    更新日期:1985-10-01 00:00:00

  • Bioconversion of m-hydroxybenzoate to 2,3-dihydroxybenzoate by mutants of Pseudomonas testosteroni.

    abstract::Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.1.293-296.1980

    authors: Daumy GO,McColl AS,Andrews GC

    更新日期:1980-01-01 00:00:00