Abstract:
:Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells at 30 degrees C but grew as spherical cells at 42 degrees C. The phenotype of one of these rod(Ts) mutants was 88% cotransducible with lip (14.3 min), and all lip+ rod(Ts) transductants of a lip recipient had the following characteristics: (i) growth was relatively sensitive to mecillinam at 30 degrees C but relatively resistant to mecillinam at 42 degrees C; (ii) penicillin-binding protein 2 was present in membranes of cells grown at 30 degrees C in reduced amounts and was undetectable in the membranes of cells grown at 42 degrees C. The mecillinam resistance, penicillin-binding protein 2 defect, and rod phenotypes all cotransduced with lip with high frequency. Thus the mutation [rodA(Ts)] is most likely in the gene for penicillin-binding protein 2 and causes the organism to grow as a sphere at 42 degrees C, although it grows with normal rodlike morphology at 30 degrees C. At 42 degrees C, cells of this strain were round with many wrinkles on their surfaces, as revealed by scanning electron microscopy. In these round cells, chromosomes were dispersed or distributed peripherally, in contrast to normal rod-shaped cells which had centrally located, more condensed chromosomes. The round cells divided asymmetrically on solid agar, and it seemed that the plane of each successive division was perpendicular to the preceding one. On temperature shift-down in liquid medium many cells with abnormal morphology appeared before normal rod-shaped cells developed. Few abnormal cells were seen when cells were placed on solid medium during temperature shift-down. These pleiotropic effects are presumably caused by one or more mutations in the rodA gene.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Iwaya M,Goldman R,Tipper DJ,Feingold B,Strominger JLdoi
10.1128/JB.136.3.1143-1158.1978subject
Has Abstractpub_date
1978-12-01 00:00:00pages
1143-58issue
3eissn
0021-9193issn
1098-5530journal_volume
136pub_type
杂志文章abstract::Streptomyces azureus carried one copy or less of plasmid pSA1, which elicited pocks at 0.1 to 1.0%. Strain PK100 was isolated from the wild-type strain after UV irradiation. PK100 carried approximately 20 to 30 copies of pSA1.1, a derivative of pSA1. Plasmid pSA1.1 elicited pocks at 100% and inhibited spore and thiost...
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pub_type: 杂志文章
doi:10.1128/jb.168.1.452-454.1986
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doi:10.1128/JB.150.1.410-413.1982
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pub_type: 杂志文章
doi:10.1128/JB.153.3.1493-1501.1983
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.403-406.1969
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.170.7.3053-3057.1988
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5619-5625.1998
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00680-12
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00642-08
更新日期:2008-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.14.4486-4491.2004
更新日期:2004-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.1054-1057.1973
更新日期:1973-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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abstract::A periplasmic protein essential for the function of the oligopeptide transport system of Salmonella typhimurium was identified. This protein, encoded by the oppA gene, is one of the most abundant proteins in the periplasm and, with an apparent molecular weight of 52,000, is considerably larger than any other known per...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1434-1438.1983
更新日期:1983-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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doi:10.1128/jb.177.18.5370-5373.1995
更新日期:1995-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.151.1.229-236.1982
更新日期:1982-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.1015-1025.1973
更新日期:1973-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.657-663.1983
更新日期:1983-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.176.21.6590-6598.1994
更新日期:1994-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.154-161.1976
更新日期:1976-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.987-996.1968
更新日期:1968-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.20.6998-7008.2005
更新日期:2005-10-01 00:00:00