Characterization of YmgF, a 72-residue inner membrane protein that associates with the Escherichia coli cell division machinery.

abstract：:Site-directed mutagenesis has been employed to address the functional significance of the highly conserved aspartic and glutamic acid residues present in the Walker B (also called motif II) sequence in Escherichia coli DNA helicase II. Two mutant proteins, UvrDE221Q and UvrDD220NE221Q, were expressed and purified to a...

journal_title：Journal of bacteriology

authors： Brosh RM Jr,Matson SW

更新日期：1995-10-01 00:00:00

abstract：:Fixation of NaH(14)CO(3) by a heavy cell suspension of Streptococcus faecalis var. liquefaciens was studied. Several nutrients, pyridoxal, riboflavine, adenine, uracil, and O(2) stimulated (14)CO(2) incorporation into cells only under conditions that were adequate for synthesis of cell macromolecules. Biotin increased...

journal_title：Journal of bacteriology

authors： Goff RC,Hartman RE

更新日期：1970-10-01 00:00:00

abstract：:A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

journal_title：Journal of bacteriology

authors： Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

更新日期：1995-07-01 00:00:00

abstract：:The bacterial response to stress is controlled by two proteins, RelA and SpoT. RelA generates the alarmone (p)ppGpp under amino acid starvation, whereas SpoT is responsible for (p)ppGpp hydrolysis and for synthesis of (p)ppGpp under a variety of cellular stress conditions. It is widely accepted that RelA is associated...

journal_title：Journal of bacteriology

authors： Jiang M,Sullivan SM,Wout PK,Maddock JR

更新日期：2007-09-01 00:00:00

abstract：:Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...

journal_title：Journal of bacteriology

authors： MacLachlan PR,Keenleyside WJ,Dodgson C,Whitfield C

更新日期：1993-12-01 00:00:00

abstract：:The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,00...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schwartz M

更新日期：1983-10-01 00:00:00

abstract：:Two chemotactic transducers for inorganic phosphate (P(i)), designated CtpH and CtpL, have been identified in Pseudomonas aeruginosa. The corresponding genes (ctpH and ctpL) were inactivated by inserting kanamycin and tetracycline resistance gene cassettes into the wild-type genes in the P. aeruginosa PAO1 genome. Com...

journal_title：Journal of bacteriology

authors： Wu H,Kato J,Kuroda A,Ikeda T,Takiguchi N,Ohtake H

更新日期：2000-06-01 00:00:00

abstract：:tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...

journal_title：Journal of bacteriology

authors： Yu N,Jora M,Solivio B,Thakur P,Acevedo-Rocha CG,Randau L,de Crécy-Lagard V,Addepalli B,Limbach PA

更新日期：2019-04-09 00:00:00

abstract：:Upon cold shock, Escherichia coli cell growth transiently stops. During this acclimation phase, specific cold shock proteins (CSPs) are highly induced. At the end of the acclimation phase, their synthesis is reduced to new basal levels, while the non-cold shock protein synthesis is resumed, resulting in cell growth re...

journal_title：Journal of bacteriology

authors： Yamanaka K,Inouye M

更新日期：2001-05-01 00:00:00

abstract：:In vivo and in vitro methylation, methanol production assays, and the use of specific antibodies raised against the sensory transducing protein Tar in Escherichia coli all failed to demonstrate the presence of methyl-accepting chemotaxis proteins (MCPs) in the photosynthetic bacterium Rhodobacter sphaeroides, although...

journal_title：Journal of bacteriology

authors： Sockett RE,Armitage JP,Evans MC

更新日期：1987-12-01 00:00:00

abstract：:In Escherichia coli, the cytoplasmic proteins MreB and FtsZ play crucial roles in ensuring that new muropeptide subunits are inserted into the cell wall in a spatially correct way during elongation and division. In particular, to retain a constant diameter and overall shape, new material must be inserted into the wall...

journal_title：Journal of bacteriology

authors： Varma A,Young KD

更新日期：2009-06-01 00:00:00

abstract：:A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four muta...

journal_title：Journal of bacteriology

authors： Van Alstyne D,Simon MI

更新日期：1971-12-01 00:00:00

abstract：:The two-component system SaeRS of Staphylococcus aureus is closely involved in the regulation of major virulence factors. However, little is known about the signals leading to saeRS activation. A total of four overlapping transcripts (T1 to T4) from three different transcription starting points are expressed in the sa...

journal_title：Journal of bacteriology

authors： Geiger T,Goerke C,Mainiero M,Kraus D,Wolz C

更新日期：2008-05-01 00:00:00

abstract：:Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activiti...

journal_title：Journal of bacteriology

authors： Habe H,Chung JS,Kato H,Ayabe Y,Kasuga K,Yoshida T,Nojiri H,Yamane H,Omori T

更新日期：2004-09-01 00:00:00

abstract：:Rough variants of serovars from the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex were isolated in high frequency from pellicle growth of the wild-type strains. Rough morphology could be correlated with the lack of an outer cell wall sheath and its constituent C-mycoside glycopept...

journal_title：Journal of bacteriology

authors： Barrow WW,Brennan PJ

更新日期：1982-04-01 00:00:00

abstract：:Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at si...

journal_title：Journal of bacteriology

authors： Stewart P,Kersten P,Vanden Wymelenberg A,Gaskell J,Cullen D

更新日期：1992-08-01 00:00:00

abstract：:Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

journal_title：Journal of bacteriology

authors： Hahn J,Dubnau D

更新日期：1985-06-01 00:00:00

abstract：:Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...

journal_title：Journal of bacteriology

authors： Karunakaran KP,Noguchi Y,Read TD,Cherkasov A,Kwee J,Shen C,Nelson CC,Brunham RC

更新日期：2003-03-01 00:00:00

abstract：:Using an auxotrophic strain of Saccharomyces cerevisiae, we examined the kinetics of ribonucleic acid (RNA) synthesis following inhibition of protein synthesis caused by amino acid starvation or cycloheximide. Removal of a required amino acid immediately stopped net protein synthesis. After a brief lag, RNA synthesis ...

journal_title：Journal of bacteriology

authors： Roth RM,Dampier C

更新日期：1972-02-01 00:00:00

abstract：:Among 120 strains of gliding bacteria which were screened for restriction endonucleases, 27 were found positive. Additionally, three strains carried enzymes able to release the supercoiled state of closed circular DNA. By using a new rapid method, restriction endonuclease activity was released by stirring about 0.5 g ...

journal_title：Journal of bacteriology

authors： Mayer H,Reichenbach H

更新日期：1978-11-01 00:00:00

abstract：:A survey of chromosomal variation in the ST239 clonal group of methicillin-resistant Staphylococcus aureus (MRSA) revealed a novel genetic element, ICE6013. The element is 13,354 bp in length, excluding a 6,551-bp Tn552 insertion. ICE6013 is flanked by 3-bp direct repeats and is demarcated by 8-bp imperfect inverted r...

journal_title：Journal of bacteriology

authors： Smyth DS,Robinson DA

更新日期：2009-10-01 00:00:00

abstract：:Lambda transducing phage gamma 16-2 carries the genes envA, ftsZ, ftsA, ddl, and murC and directs the synthesis of six unique proteins in ultraviolet-irradiated cells. Various derivatives of gamma 16-2 carrying smaller segments of the bacterial deoxyribonucleic acid have also been analyzed for their capacity to direct...

journal_title：Journal of bacteriology

authors： Lutkenhaus JF,Wu HC

更新日期：1980-09-01 00:00:00

abstract：:Lysogenization of Salmonella typhimurium with either of the bacteriophages A3 and A4 results in O-acetylation of the L-rhamnose residues of the O-polysaccharide chain of the lipopolysaccharide of the bacterial cell envelope. The O-acetyl group is found on both O-2 and O-3 of the L-rhamnosyl residues. This lysogenic co...

journal_title：Journal of bacteriology

authors： Wollin R,Stocker BA,Lindberg AA

更新日期：1987-03-01 00:00:00

abstract：:From a lysogen with lambda integrated in the leu operon, specialized transducing phages that carry the cell division, murein biosynthesis, and envelope permeability genes located about 0.5 min to the right of leu were isolated. These phages were used to identify the previously undiscovered cell division gene sep. A ge...

journal_title：Journal of bacteriology

authors： Fletcher G,Irwin CA,Henson JM,Fillingim C,Malone MM,Walker JR

更新日期：1978-01-01 00:00:00

abstract：:The flavodoxins constitute a highly conserved family of small, acidic electron transfer proteins with flavin mononucleotide prosthetic groups. They are found in prokaryotes and in red and green algae, where they provide electrons at low potentials for the reduction of nitrogen by nitrogenase, for the light-dependent r...

journal_title：Journal of bacteriology

authors： Osborne C,Chen LM,Matthews RG

更新日期：1991-03-01 00:00:00

abstract：:A polysaccharide-rich particulate fraction was isolated from cytoplasmic extracts of Candida albicans by a procedure using differential centrifugation. The polysaccharide particles obtained after purification with deoxycholate treatment were essentially free of nitrogen and were identified chemically as polyglucosan, ...

journal_title：Journal of bacteriology

authors： Yamaguchi H,Kanda Y,Iwata K

更新日期：1974-10-01 00:00:00

abstract：:The polycistronic mRNA encoding the nine genes of the unc operon of Escherichia coli was studied. We demonstrated the ribosome-binding capabilities of six of the nine unc genes, uncB, uncE, uncF, uncH, uncA, and uncD, by using the technique of primer extension inhibition or "toeprinting." No toeprint was detected for ...

journal_title：Journal of bacteriology

authors： Schaefer EM,Hartz D,Gold L,Simoni RD

更新日期：1989-07-01 00:00:00

abstract：:The inhibition of growth of the K-12 strain of Escherichia coli by glycyl-l-leucine observed originally by Simmonds and co-workers was investigated. The inhibition was reversed by isoleucine and those precursors of isoleucine beyond threonine in the biosynthetic pathway. Threonine reversed the inhibition poorly. With ...

journal_title：Journal of bacteriology

authors： Vonder Haar RA,Umbarger HE

更新日期：1972-10-01 00:00:00

abstract：:The ftsZ gene is thought to be an essential cell division gene in Escherichia coli. We constructed a null allele of ftsZ in a strain carrying additional copies of ftsZ on a plasmid with a temperature-sensitive replication defect. This strain was temperature sensitive for cell division and viability, confirming that ft...

journal_title：Journal of bacteriology

authors： Dai K,Lutkenhaus J

更新日期：1991-06-01 00:00:00

abstract：:When Methanobacterium thermoautotrophicum cells were incubated in 50 mM potassium phosphate buffer (pH 7.0) containing 1 M sucrose and autolysate from Methanobacterium wolfei, they were transformed into protoplasts. The protoplasts, which possessed no cell wall, lysed in buffer without sucrose. Unlike whole cells, the...

journal_title：Journal of bacteriology

authors： Mountfort DO,Mörschel E,Beimborn DB,Schönheit P

更新日期：1986-11-01 00:00:00