Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast.

Abstract:

:Using an auxotrophic strain of Saccharomyces cerevisiae, we examined the kinetics of ribonucleic acid (RNA) synthesis following inhibition of protein synthesis caused by amino acid starvation or cycloheximide. Removal of a required amino acid immediately stopped net protein synthesis. After a brief lag, RNA synthesis also ceased. Cycloheximide, a ribosome-inhibiting drug, also immediately halted net protein synthesis. Again RNA synthesis stopped after a brief lag. Although cycloheximide and amino acid starvation affect different steps in protein biosynthesis, both inhibited RNA synthesis in identical fashion. This indicates that amino acids do not play a unique role in the control of RNA production in rapidly growing yeast; rather, it suggests that RNA synthesis is responsive to the overall rate of protein synthesis itself.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Roth RM,Dampier C

doi

10.1128/JB.109.2.773-779.1972

subject

Has Abstract

pub_date

1972-02-01 00:00:00

pages

773-9

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

109

pub_type

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