Host cell and ultraviolet reactivation of ultraviolet-irradiated Mycoplasmaviruses.

Abstract:

:The mycoplasma Acholeplasma laidlawii was shown to have mechanisms for both host cell and ultraviolet (UV) reactivation of UV-irradiated mycoplasmaviruses. Host cell reactivation was examined by comparing the survival abilities of UV-irradiated double-stranded deoxyribonucleic acid mycoplasmavirus plated on both untreated and on acriflavine-treated cells. Acriflavine treatment inhibited cell exision repair. Decreased survival on the acriflavine-treated cells demonstrated host cell reactivation. UV reactivation was studied by comparing the survival of UV-irradiated virus plated on untreated cells with its survival on cells that received a small UV dose before plating. The UV-irradiated cells gave increased virus survival, showing UV reactivation. Similar experiments with a single-stranded deoxyribonucleic acid mycoplasmavirus showed that this virus could be UV reactivated, but not host cell reactivated.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Das J,Nowak JA,Maniloff J

doi

10.1128/JB.129.3.1424-1427.1977

subject

Has Abstract

pub_date

1977-03-01 00:00:00

pages

1424-7

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

129

pub_type

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