Salmonella typhimurium mutants with reduced levels of transfer ribonucleic acid-inhibitable endodeoxyribonucleolytic activity.

Abstract:

:Two methods of mutagenesis, chemical alkylation and insertion of the tetracycline resistance transposon Tn10, were used to generate mutants of Salmonella typhimurium which had reduced levels of endodeoxyribonucleolytic activity. The chemically induced mutations defined a locus, endA, which was cotransduced with serA at a low frequency and with metK at a high frequency. Three-factor crosses revealed that metK was between serA and endA. The major endodeoxyribonucleolytic activity in crude extracts of s. typhimurium was similar to the activity of Escherichia coli endonuclease I. A Tn10 insertion mutation of endA resulted in the most severe loss of endodeoxyribonucleolytic activity among the endA alleles studied. Two of the chemically induced mutations resulted in activities which were more thermolabile than the wild-type activity.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Zabel DJ,Trucksis M,Depew RE

doi

10.1128/JB.144.1.173-178.1980

subject

Has Abstract

pub_date

1980-10-01 00:00:00

pages

173-8

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

144

pub_type

杂志文章
  • Characterization of the detachable Rho-dependent transcription terminator of the fimE gene in Escherichia coli K-12.

    abstract::The fim genetic switch in the chromosome of Escherichia coli K-12 is an invertible DNA element that harbors the promoter for transcription of the downstream fim structural genes and a transcription terminator that acts on the upstream fimE regulatory gene. Switches oriented appropriately for structural gene transcript...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.24.8256-8266.2005

    authors: Hinde P,Deighan P,Dorman CJ

    更新日期:2005-12-01 00:00:00

  • MICROBIAL METABOLISM OF AROMATIC COMPOUNDS. I. DECOMPOSITION OF PHENOLIC COMPOUNDS AND AROMATIC HYDROCARBONS BY PHENOL-ADAPTED BACTERIA.

    abstract::Tabak, Henry H. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Cecil W. Chambers, and Paul W. Kabler. Microbial metabolism of aromatic carbon compounds. I. Decomposition of phenolic compounds and aromatic hydrocarbons by phenol-adapted bacteria. J. Bacteriol. 87:910-919. 1964.-Bacteria from soil and r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.4.910-919.1964

    authors: TABAK HH,CHAMBERS CW,KABLER PW

    更新日期:1964-04-01 00:00:00

  • Plasmid-to-chromosome gene transfer in Haemophilus influenza during growth.

    abstract::A low level of recombination between homologous regions of plasmids and the host chromosome occurred during cell growth. The plasmids contained antibiotic resistance markers on the homologous regions which were only expressed when they were integrated into the chromosome. Such recombination took place in Rec+ rec-2 mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.1.458-459.1986

    authors: Balganesh M,Arrigoni L,Setlow JK

    更新日期:1986-10-01 00:00:00

  • Substrate recognition properties of oligopeptidase B from Salmonella enterica serovar Typhimurium.

    abstract::Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.12.3329-3337.2002

    authors: Morty RE,Fülöp V,Andrews NW

    更新日期:2002-06-01 00:00:00

  • Global gene expression analysis of the heat shock response in the phytopathogen Xylella fastidiosa.

    abstract::Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00182-06

    authors: Koide T,Vêncio RZ,Gomes SL

    更新日期:2006-08-01 00:00:00

  • The bkdR gene of Pseudomonas putida is required for expression of the bkd operon and encodes a protein related to Lrp of Escherichia coli.

    abstract::Branched-chain keto acid dehydrogenase is a multienzyme complex which is required for the metabolism of the branched-chain amino acids in Pseudomonas putida. The structural genes encoding all four proteins of the bkd operon have been cloned, and their nucleotide sequences have been determined (G. Burns, K. T. Madhusud...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.13.3934-3940.1993

    authors: Madhusudhan KT,Lorenz D,Sokatch JR

    更新日期:1993-07-01 00:00:00

  • HK022 Nun Requires Arginine-Rich Motif Residues Distinct from λ N.

    abstract:UNLABELLED:Bacteriophage λ N protein binds boxB RNA hairpins in the nut (N utilization) sites of immediate early λ transcripts and interacts with host factors to suppress transcriptional termination at downstream terminators. In opposition to λ N, the Nun protein of HK022 binds the boxBs of coinfecting λ transcripts, i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00466-15

    authors: Tawk CS,Ghattas IR,Smith CA

    更新日期:2015-11-01 00:00:00

  • Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli.

    abstract::Tn5 insertion mutations in the recN gene, and in what appears to be a new RecF pathway gene designated recO and mapping at approximately 55.4 min on the standard genetic map, were isolated by screening Tn5 insertion mutations that cotransduced with tyrA. The recO1504::Tn5 mutation decreased the frequency of recombinat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.3.1060-1066.1985

    authors: Kolodner R,Fishel RA,Howard M

    更新日期:1985-09-01 00:00:00

  • Medium-dependent alteration of lysine transfer ribonucleic acid in sporulating Bacillus subtilis cells.

    abstract::The presence of the altered lysine transfer ribonucleic acid (tRNA) in Bacillus subtilis spores is strongly dependent on the medium on which the cells were sporulated. Cells sporulated on synthetic media or dilute complex media contain little or none of the new component, whereas those sporulated on concentrated compl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.125-130.1967

    authors: Lazzarini RA,Santangelo E

    更新日期:1967-07-01 00:00:00

  • Sequence of molecular events involved in induction of allophanate hydrolase.

    abstract::Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic acid (RNA) and protein synthesis are needed for this induction to occur. To elucidate the time intervals occupied by kno...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.1.198-204.1976

    authors: Bossinger J,Cooper TG

    更新日期:1976-04-01 00:00:00

  • Conservation of genetic linkage in nonisogenic isolates of Candida albicans.

    abstract::A number of laboratories are now engaged in the genetic analysis of Candida albicans. This diploid yeast, the major fungal pathogen of humans, is imperfect. Parasexual techniques have been devised for complementation and recombination analysis in this organism. This paper attempts to address the question of the extent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.2.498-506.1983

    authors: Poulter R,Hanrahan V

    更新日期:1983-11-01 00:00:00

  • The Moraxella catarrhalis immunoglobulin D-binding protein MID has conserved sequences and is regulated by a mechanism corresponding to phase variation.

    abstract::The prevalence of the Moraxella catarrhalis immunoglobulin D (IgD)-binding outer membrane protein MID and its gene was determined in 91 clinical isolates and in 7 culture collection strains. Eighty-four percent of the clinical Moraxella strains expressed MID-dependent IgD binding. The mid gene was detected in all stra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.7.2285-2295.2003

    authors: Möllenkvist A,Nordström T,Halldén C,Christensen JJ,Forsgren A,Riesbeck K

    更新日期:2003-04-01 00:00:00

  • Influences of capsule on cell shape and chain formation of wild-type and pcsB mutants of serotype 2 Streptococcus pneumoniae.

    abstract::PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01505-08

    authors: Barendt SM,Land AD,Sham LT,Ng WL,Tsui HC,Arnold RJ,Winkler ME

    更新日期:2009-05-01 00:00:00

  • Draft genome sequences for two metal-reducing Pelosinus fermentans strains isolated from a Cr(VI)-contaminated site and for type strain R7.

    abstract::Pelosinus fermentans 16S rRNA gene sequences have been reported from diverse geographical sites since the recent isolation of the type strain. We present the genome sequence of the P. fermentans type strain R7 (DSM 17108) and genome sequences for two new strains with different abilities to reduce iron, chromate, and u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01174-12

    authors: Brown SD,Podar M,Klingeman DM,Johnson CM,Yang ZK,Utturkar SM,Land ML,Mosher JJ,Hurt RA Jr,Phelps TJ,Palumbo AV,Arkin AP,Hazen TC,Elias DA

    更新日期:2012-09-01 00:00:00

  • Loss of 4.5S RNA induces the heat shock response and lambda prophage in Escherichia coli.

    abstract::During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.1151-1154.1990

    authors: Bourgaize DB,Phillips TA,VanBogelen RA,Jones PG,Neidhardt FC,Fournier MJ

    更新日期:1990-02-01 00:00:00

  • Regulation of the transposase of Tn4652 by the transposon-encoded protein TnpC.

    abstract::Transposition is a DNA reorganization reaction potentially deleterious for the host. The frequency of transposition is limited by the amount of transposase. Therefore, strict regulation of a transposase is required to keep control over the destructive multiplication of the mobile element. We have shown previously that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6312-6318.1999

    authors: Hõrak R,Kivisaar M

    更新日期:1999-10-01 00:00:00

  • Host cell and ultraviolet reactivation of ultraviolet-irradiated Mycoplasmaviruses.

    abstract::The mycoplasma Acholeplasma laidlawii was shown to have mechanisms for both host cell and ultraviolet (UV) reactivation of UV-irradiated mycoplasmaviruses. Host cell reactivation was examined by comparing the survival abilities of UV-irradiated double-stranded deoxyribonucleic acid mycoplasmavirus plated on both untre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1424-1427.1977

    authors: Das J,Nowak JA,Maniloff J

    更新日期:1977-03-01 00:00:00

  • Stimulation of deletions in the Escherichia coli chromosome by partially induced Mucts62 prophages.

    abstract::Deletion of bacterial DNA fragments is stimulated in induced Mucts62 lysogens. The host genes located proximally to the prophage are more frequently lost than those which are unlinked to the Mu genome. Genes located on either side of a Mu genome are deleted in the same manner. Like the other Mu-induced rearrangements,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.2.477-483.1978

    authors: Faelen M,Toussaint A

    更新日期:1978-11-01 00:00:00

  • Role of the ganSPQAB Operon in Degradation of Galactan by Bacillus subtilis.

    abstract::Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00468-16

    authors: Watzlawick H,Morabbi Heravi K,Altenbuchner J

    更新日期:2016-09-22 00:00:00

  • Polypeptide involved in the Escherichia coli plasmid-mediated citrate transport system.

    abstract::A genetic determinant conferring on Escherichia coli the ability to utilize citrate as a sole source of carbon and energy was subcloned into pBR322 from a naturally occurring, citrate utilization (Cit+) plasmid, pOH30221, and was localized to a 1.6-kilobase region by cloning and subsequent deletion analysis. Genetic e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.421-426.1984

    authors: Hirato T,Shinagawa M,Ishiguro N,Sato G

    更新日期:1984-10-01 00:00:00

  • Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha.

    abstract::Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the ina...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.2.446-452.1986

    authors: Chandra TS,Friedrich CG

    更新日期:1986-05-01 00:00:00

  • Coordination of Synthesis and Assembly of a Modular Membrane-Associated [NiFe]-Hydrogenase Is Determined by Cleavage of the C-Terminal Peptide.

    abstract:UNLABELLED:During biosynthesis of [NiFe]-hydrogenase 2 (Hyd-2) of Escherichia coli, a 15-amino-acid C-terminal peptide is cleaved from the catalytic large subunit precursor, pro-HybC. This peptide is removed only after NiFe(CN)2CO cofactor insertion by the Hyp accessory protein machinery has been completed, suggesting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00437-15

    authors: Thomas C,Muhr E,Sawers RG

    更新日期:2015-09-01 00:00:00

  • Denaturation mapping of R factor deoxyribonucleic acid.

    abstract::The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it is possible to distinguish the RTF-TC region from the r-determinants r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.1035-1042.1975

    authors: Perlman D,Twose TM,Holland MJ,Rownd RH

    更新日期:1975-09-01 00:00:00

  • Inhibition of secretion of a mutant lipoprotein across the cytoplasmic membrane by the wild-type lipoprotein of the Escherichia coli outer membrane.

    abstract::A globomycin-resistant mutant of Escherichia coli was found to produce a precursor of the major outer membrane lipoprotein (prolipoprotein), in which the glycine residue at position 14 within the signal peptide was replaced by an aspartic acid residue. The same mutation has been reported by Lin et al. (Proc. Natl. Aca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.1.407-411.1983

    authors: Lee N,Yamagata H,Inouye M

    更新日期:1983-07-01 00:00:00

  • Protein synthesis during fungal spore germination. II. Aminoacyl-soluble ribonucleic acid synthetase activities during germination of Botryodiplodia theobromae spores.

    abstract::The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.4.1042-1048.1968

    authors: Van Etten JL,Brambl RM

    更新日期:1968-10-01 00:00:00

  • Molecular analysis of the Escherichia coli phoP-phoQ operon.

    abstract::The phoP-phoQ operon of Salmonella typhimurium is a member of the family of two-component regulatory systems and controls expression of the phoN gene that codes for nonspecific acid phosphatase and the genes involved in the pathogenicity of the bacterium. The phoP-phoQ operon of Escherichia coli was cloned on a plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.2.492-498.1992

    authors: Kasahara M,Nakata A,Shinagawa H

    更新日期:1992-01-01 00:00:00

  • Pyrogenic principle of the ribonucleic acid from the yeast Candida utilis.

    abstract::Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chrom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.3.738-743.1968

    authors: Kanoh S,Kohlhage H,Siegert R

    更新日期:1968-09-01 00:00:00

  • Mechanism of action of the antifungal antibiotic pyrrolnitrin.

    abstract::Pyrrolnitrin at 10 mug/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.310-318.1969

    authors: Tripathi RK,Gottlieb D

    更新日期:1969-10-01 00:00:00

  • Growth phase variation in cell and nucleoid morphology in a Bacillus subtilis recA mutant.

    abstract::The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects. However, the efficiency of plating was about 12% of that of the parent strain. A substantial and additive d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.9.2963-2968.2001

    authors: Sciochetti SA,Blakely GW,Piggot PJ

    更新日期:2001-05-01 00:00:00

  • Multiple loci of Pseudomonas syringae pv. syringae are involved in pathogenicity on bean: restoration of one lesion-deficient mutant requires two tRNA genes.

    abstract::A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.7.2247-2258.1997

    authors: Rich JJ,Willis DK

    更新日期:1997-04-01 00:00:00