Abstract:
:The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extracts of the ungerminated spores were prepared with mortar and pestle, very little or no enzyme activity was detected. When the extracts were prepared with a mechanical homogenizer, however, they exhibited some enzyme activity, although less than did the extracts from germinated spores. Enzyme activities from germinated spores were approximately the same, regardless of the method of preparation. The enzyme fraction from ungerminated spores prepared with a mechanical homogenizer could also stimulate incorporation of phenylalanine into polyphenylalanine in the presence of ribosomes, polyuridylic acid, and sRNA, although the activity was approximately only 15 to 20% that of a similar enzyme fraction from germinated spores. It is concluded that ungerminated spores of B. theobromae contain active aminoacyl-sRNA synthetases and transfer enzymes, although the activities are low when compared to germinated spores.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Van Etten JL,Brambl RMdoi
10.1128/JB.96.4.1042-1048.1968subject
Has Abstractpub_date
1968-10-01 00:00:00pages
1042-8issue
4eissn
0021-9193issn
1098-5530journal_volume
96pub_type
杂志文章abstract::The bacterium Myxococcus xanthus alternates between two colony types distinguished by colony morphology and pigmentation. Because the two phases are interconvertible, this phenomenon has been termed phase variation. In one phase, the colonies are bright yellow, rough, and swarming. In the alternate phase, the colonies...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.17.5341-5349.1994
更新日期:1994-09-01 00:00:00
abstract::A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1186-1189.1974
更新日期:1974-06-01 00:00:00
abstract::A second cluster of genes encoding the E1 alpha, E1 beta, and E2 subunits of branched-chain alpha-keto acid dehydrogenase (BCDH), bkdFGH, has been cloned and characterized from Streptomyces avermitilis, the soil microorganism which produces anthelmintic avermectins. Open reading frame 1 (ORF1) (bkdF, encoding E1 alpha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.12.3504-3511.1995
更新日期:1995-06-01 00:00:00
abstract::Cells of Bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. The autolysate contained enzymes which lysed vegetative cell walls of the organism. Three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. One enzyme, most active near pH 4.0,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.3.629-638.1968
更新日期:1968-09-01 00:00:00
abstract::Lactobacillus brevis transports glucose and the nonmetabolizable glucose analog 2-deoxyglucose via a proton symport mechanism that is allosterically inhibited by the seryl-phosphorylated derivative of HPr, the small phosphocarrier protein of the phosphotransferase system. We have demonstrate that S46DHPr, a mutant ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.7.1900-1902.1995
更新日期:1995-04-01 00:00:00
abstract::We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01415-09
更新日期:2010-03-01 00:00:00
abstract::The metabolism of mRNA from the lactose (lac) operon of Escherichia coli has been studied in ribonuclease (RNase) III-deficient strains (rnc-105). The induction lag for beta-galactosidase from the first gene was twice as long, and enzyme synthesis was reduced 10-fold in one such mutant compared with its isogenic rnc+ ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.528-541.1978
更新日期:1978-08-01 00:00:00
abstract::A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.3.1144-1155.1973
更新日期:1973-03-01 00:00:00
abstract:UNLABELLED:The latent period of phage T4, normally ∼25 min, can be extended indefinitely if the infected cell is superinfected after 5 min. This phenomenon, designated lysis inhibition (LIN), was first described in the 1940s and is genetically defined by mutations in diverse T4 r genes. RI, the main effector of LIN, ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00294-16
更新日期:2016-08-25 00:00:00
abstract::ibeA is a virulence factor found in some extraintestinal pathogenic Escherichia coli (ExPEC) strains from the B2 phylogenetic group and particularly in newborn meningitic and avian pathogenic strains. It was shown to be involved in the invasion process of the newborn meningitic strain RS218. In a previous work, we sho...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00089-12
更新日期:2012-09-01 00:00:00
abstract::Haemophilus influenzae is a common gram-negative pathogen that initiates infection by colonizing the upper respiratory tract epithelium. In previous work, we reported the isolation of a locus involved in expression of short, thin surface fibrils by H. influenzae type b and presented evidence that surface fibrils promo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.21.6281-6287.1996
更新日期:1996-11-01 00:00:00
abstract::Slein, Milton W. (Fort Detrick, Frederick, Md.) and Gerald F. Logan, Jr. Mechanism of action of the toxin of Bacillus anthracis. II. Alkaline phosphatasemia produced by culture filtrates of various bacilli. J. Bacteriol. 83:359-369. 1962.-A factor which produces hyperphosphatasemia after intravenous injection into ani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.359-369.1962
更新日期:1962-02-01 00:00:00
abstract::Apparent pK values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at pH 7 in media free from nitrogen, sulfur, and phosphorus. Of the compounds possessing pK' values near pH 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (EMTA) was c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.2.283-290.1967
更新日期:1967-08-01 00:00:00
abstract::The Yersinia adhesin A (YadA) is a trimeric autotransporter adhesin of enteric yersiniae. It consists of three major domains: a head mediating adherence to host cells, a stalk involved in serum resistance, and an anchor that forms a membrane pore and is responsible for the autotransport function. The anchor contains a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00985-07
更新日期:2007-12-01 00:00:00
abstract::Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.425-427.1980
更新日期:1980-10-01 00:00:00
abstract::Purified suspensions of Chlamydia psittaci were prepared from L cells. Thin sections of intact elementary bodies and intact developmental reticulate bodies and of their purified envelopes were observed by electron microscopy. In both intact organisms and partially purified envelopes, two membranous structures, each ap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.355-360.1971
更新日期:1971-01-01 00:00:00
abstract::The ultimate membrane localization and function of most of the 185 predicted Pseudomonas aeruginosa PAO1 lipoproteins remain unknown. We constructed a fluorescent lipoprotein, CSFP(OmlA)-ChFP, by fusing the signal peptide and the first four amino acids of the P. aeruginosa outer membrane lipoprotein OmlA to the monome...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00603-08
更新日期:2008-09-01 00:00:00
abstract::The involvement of the recently characterized 5' exonuclease activity of RNase J1 and endonuclease activity of RNase Y in the turnover of ΔermC mRNA in Bacillus subtilis was investigated. Evidence is presented that both of these activities determine the half-life of ΔermC mRNA. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05939-11
更新日期:2011-11-01 00:00:00
abstract::Sphingobium japonicum UT26 utilizes gamma-hexachlorocyclohexane (gamma-HCH) as its sole source of carbon and energy. In our previous studies, we cloned and characterized genes encoding enzymes for the conversion of gamma-HCH to beta-ketoadipate in UT26. In this study, we analyzed a mutant obtained by transposon mutage...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01883-06
更新日期:2007-05-01 00:00:00
abstract:UNLABELLED:Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02564-14
更新日期:2015-05-01 00:00:00
abstract::Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.3.1033-1039.1972
更新日期:1972-12-01 00:00:00
abstract::A pattern of allosteric control for aromatic biosynthesis in cyanobacteria relies upon early-pathway regulation as the major control point for the entire branched pathway. In Synechococcus sp. strain PCC6301 (Anacystis nidulans), two enzymes which form precursors for L-phenylalanine biosynthesis are subject to control...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.423-428.1983
更新日期:1983-01-01 00:00:00
abstract::Estrada-Parra, Sergio (Escuela Nacional de Ciencias Biológicas, México, D.F., México), Abel Zamora, and L. F. Bojalil. Immunochemistry of the group-specific polysaccharide of Nocardia brasiliensis. J. Bacteriol. 90:571-574. 1965.-The group-specific polysaccharide of Nocardia brasiliensis was further purified, yielding...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.571-574.1965
更新日期:1965-09-01 00:00:00
abstract::We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2612-2619.1992
更新日期:1992-04-01 00:00:00
abstract::Coxiella burnetii is a Gram-negative, obligate intracellular bacterial pathogen that resides within the harsh, acidic confines of a lysosome-like compartment of the host cell that is termed a parasitophorous vacuole. In this study, we characterized a thiol-specific peroxidase of C. burnetii that belongs to the atypica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01324-09
更新日期:2010-04-01 00:00:00
abstract::The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.283-290.1984
更新日期:1984-01-01 00:00:00
abstract::Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type popula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3915-3923.1988
更新日期:1988-09-01 00:00:00
abstract::Tetragenococcus halophila D10 catalyzes the decarboxylation of L-aspartate with nearly stoichiometric release of L-alanine and CO(2). This trait is encoded on a 25-kb plasmid, pD1. We found in this plasmid a putative asp operon consisting of two genes, which we designated aspD and aspT, encoding an L-aspartate-beta-de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.11.2906-2913.2002
更新日期:2002-06-01 00:00:00
abstract::Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00747-09
更新日期:2009-11-01 00:00:00
abstract::After treatment with N-methyl-N'-nitro-N-nitrosoguanidine, 133 independent mutants of a haploid strain of Escherichia coli able to use phenyl-beta-galactoside as a carbon source were obtained. The galactoside was specific in selecting for mutants with increases in their uninduced levels of beta-galactosidase. Virtuall...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.500-507.1974
更新日期:1974-08-01 00:00:00