Abstract:
:Sphingobium japonicum UT26 utilizes gamma-hexachlorocyclohexane (gamma-HCH) as its sole source of carbon and energy. In our previous studies, we cloned and characterized genes encoding enzymes for the conversion of gamma-HCH to beta-ketoadipate in UT26. In this study, we analyzed a mutant obtained by transposon mutagenesis and identified and characterized new genes encoding a putative ABC-type transporter essential for the utilization of gamma-HCH in strain UT26. This putative ABC transporter consists of four components, permease, ATPase, periplasmic protein, and lipoprotein, encoded by linK, linL, linM, and linN, respectively. Mutation and complementation analyses indicated that all the linKLMN genes are required, probably as a set, for gamma-HCH utilization in UT26. Furthermore, the mutant cells deficient in this putative ABC transporter showed (i) higher gamma-HCH degradation activity and greater accumulation of the toxic dead-end product 2,5-dichlorophenol (2,5-DCP), (ii) higher sensitivity to 2,5-DCP itself, and (iii) higher permeability of hydrophobic compounds than the wild-type cells. These results strongly suggested that LinKLMN are involved in gamma-HCH utilization by controlling membrane hydrophobicity. This study clearly demonstrated that a cellular factor besides catabolic enzymes and transcriptional regulators is essential for utilization of xenobiotic compounds in bacterial cells.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Endo R,Ohtsubo Y,Tsuda M,Nagata Ydoi
10.1128/JB.01883-06subject
Has Abstractpub_date
2007-05-01 00:00:00pages
3712-20issue
10eissn
0021-9193issn
1098-5530pii
JB.01883-06journal_volume
189pub_type
杂志文章abstract::The pyrimidine de novo biosynthesis pathway has been characterized for a number of organisms. The general pathway consists of six enzymatic steps. In the characterization of the pyrimidine pathway of Lactococcus lactis, two different pyrD genes encoding dihydroorotate dehydrogenase were isolated. The nucleotide sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.13.3975-3982.1994
更新日期:1994-07-01 00:00:00
abstract::Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...
journal_title:Journal of bacteriology
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doi:10.1128/JB.83.3.500-506.1962
更新日期:1962-03-01 00:00:00
abstract::Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....
journal_title:Journal of bacteriology
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doi:10.1128/JB.84.4.793-796.1962
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/jb.173.23.7719-7722.1991
更新日期:1991-12-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.176.2.443-449.1994
更新日期:1994-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-02-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1968-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.16.5009-5013.1997
更新日期:1997-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-04-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.91.5.1869-1874.1966
更新日期:1966-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1995-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1986-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1968-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.157.3.868-873.1984
更新日期:1984-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3500-3507.1994
更新日期:1994-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.179.5.1774-1779.1997
更新日期:1997-03-01 00:00:00
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更新日期:2003-01-01 00:00:00