Instability of pathogenicity islands in uropathogenic Escherichia coli 536.

Abstract:

:The uropathogenic Escherichia coli strain 536 carries at least five genetic elements on its chromosome that meet all criteria characteristic of pathogenicity islands (PAIs). One main feature of these distinct DNA regions is their instability. We applied the so-called island-probing approach and individually labeled all five PAIs of E. coli 536 with the counterselectable marker sacB to evaluate the frequency of PAI-negative colonies under the influence of different environmental conditions. Furthermore, we investigated the boundaries of these PAIs. According to our experiments, PAI II536 and PAI III536 were the most unstable islands followed by PAI I536 and PAI V536, whereas PAI IV536 was stable. In addition, we found that deletion of PAI II536 and PAI III536 was induced by several environmental stimuli. Whereas excision of PAI I536, PAI II536, and PAI V536 was based on site-specific recombination between short direct repeat sequences at their boundaries, PAI III536 was deleted either by site-specific recombination or by homologous recombination between two IS100-specific sequences. In all cases, deletion is thought to lead to the formation of nonreplicative circular intermediates. Such extrachromosomal derivatives of PAI II536 and PAI III536 were detected by a specific PCR assay. Our data indicate that the genome content of uropathogenic E. coli can be modulated by deletion of PAIs.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Middendorf B,Hochhut B,Leipold K,Dobrindt U,Blum-Oehler G,Hacker J

doi

10.1128/jb.186.10.3086-3096.2004

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

3086-96

issue

10

eissn

0021-9193

issn

1098-5530

journal_volume

186

pub_type

杂志文章
  • Rapid viability loss on exposure to air in a superoxide dismutase-deficient mutant of Porphyromonas gingivalis.

    abstract::Porphyromonas gingivalis, an obligate anaerobe, exhibits a relatively high degree of aerotolerance and possesses superoxide dismutase (SOD) which is induced by exposure to air. To clarify roles for SOD in this organism, the gene encoding SOD (sod) on the P. gingivalis chromosome was disrupted in a gene-directed way by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.7.1939-1943.1994

    authors: Nakayama K

    更新日期:1994-04-01 00:00:00

  • Formation of the chlorophyll precursor delta-aminolevulinic acid in cyanobacteria requires aminoacylation of a tRNAGlu species.

    abstract::In the chloroplasts of higher plants and algae, the biosynthesis of the chlorophyll precursor delta-aminolevulinic acid (ALA) involves at least three enzymes and a tRNA species. Here we demonstrate that in cell extracts of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 ALA was formed from glutamate i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.3810-3816.1988

    authors: O'Neill GP,Peterson DM,Schön A,Chen MW,Söll D

    更新日期:1988-09-01 00:00:00

  • Characterization of the Escherichia coli SecA signal peptide-binding site.

    abstract::SecA signal peptide interaction is critical for initiating protein translocation in the bacterial Sec-dependent pathway. Here, we have utilized the recent nuclear magnetic resonance (NMR) and Förster resonance energy transfer studies that mapped the location of the SecA signal peptide-binding site to design and isolat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06150-11

    authors: Grady LM,Michtavy J,Oliver DB

    更新日期:2012-01-01 00:00:00

  • Dual control of sbo-alb operon expression by the Spo0 and ResDE systems of signal transduction under anaerobic conditions in Bacillus subtilis.

    abstract::The Bacillus subtilis sbo-alb operon contains sboA, the structural gene for the bacteriocin subtilosin, and the alb genes required for subtilosin production. Transcription from the sbo-alb promoter is highly induced by oxygen limitation. The transcriptional regulation of the sbo-alb operon is under dual control involv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.11.3274-3277.2000

    authors: Nakano MM,Zheng G,Zuber P

    更新日期:2000-06-01 00:00:00

  • Antigenic sites on porin of Haemophilus influenzae type b: mapping with synthetic peptides and evaluation of structure predictions.

    abstract::The major surface-located protein in the outer membrane of Haemophilus influenzae type b (Hib) is porin, molecular mass, 38 kDa, 341 amino acids. To define precisely the molecular reactivities of nine mouse monoclonal antibodies (MAbs) against Hib porin, overlapping hexapeptides corresponding to the entire sequence of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.12.4007-4016.1992

    authors: Srikumar R,Dahan D,Gras MF,Ratcliffe MJ,van Alphen L,Coulton JW

    更新日期:1992-06-01 00:00:00

  • Metronidazole activation and isolation of Clostridium acetobutylicum electron transport genes.

    abstract::An Escherichia coli F19 recA, nitrate reductase-deficient mutant was constructed by transposon mutagenesis and shown to be resistant to metronidazole. This mutant was a most suitable host for the isolation of Clostridium acetobutylicum genes on recombinant plasmids, which activated metronidazole and rendered the E. co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.3.1088-1095.1991

    authors: Santangelo JD,Jones DT,Woods DR

    更新日期:1991-02-01 00:00:00

  • Facilitated diffusion of monosaccharides in Saccharomyces cerevisiae: experimental investigation of kinetic parameters without the assumptions of symmetry.

    abstract::Until the question of symmetry or asymmetry in the facilitated diffusion of monosaccharides by Saccharomyces cerevisiae is resolved, attempts to study the transport process cannot be based on assumptions of symmetry, such as equal concentrations at equilibrium or kinetic parameters that are equal in opposite direction...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.612-618.1973

    authors: Kalsow CM,Doyle RJ

    更新日期:1973-02-01 00:00:00

  • Penicillin acyltransferase in Penicillium chrysogenum.

    abstract::Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.5.1502-1508.1967

    authors: Pruess DL,Johnson MJ

    更新日期:1967-11-01 00:00:00

  • NOVEL Escherichia coli dnaB mutant: direct involvement of the dnaB252 gene product in the synthesis of an origin-ribonucleic acid species during initiaion of a round of deoxyribonucleic acid replication.

    abstract::The initiation process of deoxyribonucleic acid (DNA) replication in Escherichia coli has been studied using the thermoreversible dna initiation mutant E. coli HfrHl65/120/6 dna-252. This dna mutation was incorrectly classed as a dnaA mutation. Biochemical and genetic evidence suggests that the dna-252 mutant is a nov...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1476-1486.1977

    authors: Zyskind JW,Smith DW

    更新日期:1977-03-01 00:00:00

  • Discovery of a nonclassical siderophore, legiobactin, produced by strains of Legionella pneumophila.

    abstract::The mechanisms by which Legionella pneumophila, a facultative intracellular parasite and the agent of Legionnaires' disease, acquires iron are largely unexplained. Several earlier studies indicated that L. pneumophila does not elaborate siderophores. However, we now present evidence that supernatants from L. pneumophi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.3.749-757.2000

    authors: Liles MR,Scheel TA,Cianciotto NP

    更新日期:2000-02-01 00:00:00

  • Chemical analysis of and degradation studies on the cell wall lipopolysaccharide of Rhodopseudomonas capsulata.

    abstract::A lipopolysaccharide (LPS) has been isolated from the gram-negative photosynthetic bacterium Rhodopseudomonas capsulata. Chemical analysis revealed the presence of d-glucose, d-galactose, l-rhamnose, 3-O-methyl-l-rhamnose (l-acofriose), d-glucosamine, 2-keto-3-deoxyoctonate, and neuraminic acid. The LPS does not conta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.3.1106-1113.1972

    authors: Weckesser J,Drews G,Fromme I

    更新日期:1972-03-01 00:00:00

  • Role of the catabolite activator protein in the maltose regulon of Escherichia coli.

    abstract::The maltose regulon consists of three operons controlled by a positive regulatory gene, malT. Deletions of the gene crp were introduced into strains which carried a malT-lacZ hybrid gene. From the observed reduction in beta-galactosidase activity it was concluded that the expression of malT-lacZ, and therefore of malT...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.2.722-729.1982

    authors: Chapon C

    更新日期:1982-05-01 00:00:00

  • Regulation of nitrogenase gene expression by transcript stability in the cyanobacterium Anabaena variabilis.

    abstract::The nitrogenase gene cluster in cyanobacteria has been thought to comprise multiple operons; however, in Anabaena variabilis, the promoter for the first gene in the cluster, nifB1, appeared to be the primary promoter for the entire nif cluster. The structural genes nifHDK1 were the most abundant transcripts; however, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02045-14

    authors: Pratte BS,Thiel T

    更新日期:2014-10-01 00:00:00

  • Escherichia coli produces linoleic acid during late stationary phase.

    abstract::Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5324-5328.1993

    authors: Rabinowitch HD,Sklan D,Chace DH,Stevens RD,Fridovich I

    更新日期:1993-09-01 00:00:00

  • Exogenous Alginate Protects Staphylococcus aureus from Killing by Pseudomonas aeruginosa.

    abstract::Cystic fibrosis (CF) patients chronically infected with both Pseudomonas aeruginosa and Staphylococcus aureus have worse health outcomes than patients who are monoinfected with either P. aeruginosa or S. aureus We showed previously that mucoid strains of P. aeruginosa can coexist with S. aureusin vitro due to the tran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00559-19

    authors: Price CE,Brown DG,Limoli DH,Phelan VV,O'Toole GA

    更新日期:2020-03-26 00:00:00

  • Glucose transport by a mutant of Streptococcus mutans unable to accumulate sugars via the phosphoenolpyruvate phosphotransferase system.

    abstract::Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS). Earlier studies indicated that an alternate glucose transport system functions in this organism under conditions of high growth rates, low pH, or excess glucose. To identify this system, S. mutans...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.9.2251-2258.1995

    authors: Cvitkovitch DG,Boyd DA,Thevenot T,Hamilton IR

    更新日期:1995-05-01 00:00:00

  • Characterization of the nonenzymatic chloramphenicol resistance (cmlA) gene of the In4 integron of Tn1696: similarity of the product to transmembrane transport proteins.

    abstract::Integrons constitute a novel family of DNA elements which evolved by site-specific integration of discrete units between two conserved segments. On the In4 integron of Tn1696, a precisely inserted gene cassette of 1,549 bp conferring nonenzymatic chloramphenicol resistance (cmlA) is present between the streptomycin-sp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.14.4493-4502.1991

    authors: Bissonnette L,Champetier S,Buisson JP,Roy PH

    更新日期:1991-07-01 00:00:00

  • MucA-mediated coordination of type III secretion and alginate synthesis in Pseudomonas aeruginosa.

    abstract::The type III secretion system (T3SS) of Pseudomonas aeruginosa is an important virulence factor. The T3SS of P. aeruginosa can be induced by a low calcium signal or upon direct contact with the host cells. The exact pathway of signal sensing and T3SS activation is not clear. By screening a transposon insertion mutant ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.22.7575-7585.2004

    authors: Wu W,Badrane H,Arora S,Baker HV,Jin S

    更新日期:2004-11-01 00:00:00

  • Function of alpha-D-glucosyl monophosphorylpolyprenol in biosynthesis of cell wall teichoic acids in Bacillus coagulans.

    abstract::D-[alpha-14C]]glucosyl phosphorylpolyprenol ([ 14C]Glc-P-prenol) was formed from UDP-D-[14C]glucose in each of the membrane systems obtained from Bacillus coagulans AHU 1631 and AHU 1634 and two Bacillus megaterium strains. Membranes of these B. coagulans strains, which possess beta-D-glucosyl branches on the repeatin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2835-2841.1989

    authors: Shimada A,Tamatukuri J,Ito E

    更新日期:1989-05-01 00:00:00

  • Global genomic analysis of Pseudomonas savastanoi pv. savastanoi plasmids.

    abstract::Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of informat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01067-07

    authors: Pérez-Martínez I,Zhao Y,Murillo J,Sundin GW,Ramos C

    更新日期:2008-01-01 00:00:00

  • Suppression of replication-deficient mutants of IncFII plasmid NR1 can occur by two different mechanisms that increase expression of the repA1 gene.

    abstract::Replication-proficient (Rep+) revertants were isolated from mutants of IncFII plasmid NR1 that were replication defective (Rep-). The parental Rep- plasmids contained a mutation that inactivated promoter PE for transcription of RNA-E, a trans-acting repressor of translation of the essential RepA1 replication initiatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.3161-3173.1993

    authors: Wu R,Wang X,Womble DD,Rownd RH

    更新日期:1993-05-01 00:00:00

  • Induction of superoxide dismutases in Escherichia coli by manganese and iron.

    abstract::Growth of Escherichia coli B in simple media enriched with Mn(II) resulted in the elevation of the manganese-containing superoxide dismutase, whereas growth in such medium enriched with iron caused increased content of the iron-containing superoxide dismutase. Enrichment of the medium with Co(II), Cu(II), Mo(VI), Zn(I...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.137-142.1984

    authors: Pugh SY,DiGuiseppi JL,Fridovich I

    更新日期:1984-10-01 00:00:00

  • EXPERIMENTAL VIABLE VACCINE AGAINST PULMONARY COCCIDIOIDOMYCOSIS IN MONKEYS.

    abstract::Converse, John L. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), Merida W. Castleberry, and Ernest M. Snyder. Experimental viable vaccine against pulmonary coccidioidomycosis in monkeys. J. Bacteriol. 86:1041-1051. 1963.-Monkeys (Macaca mulatta) vaccinated by subcutaneous injection in the forearm w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.5.1041-1051.1963

    authors: CONVERSE JL,CASTLEBERRY MW,SNYDER EM

    更新日期:1963-11-01 00:00:00

  • Genome sequence of a recently emerged, highly transmissible, multi-antibiotic- and antiseptic-resistant variant of methicillin-resistant Staphylococcus aureus, sequence type 239 (TW).

    abstract::The 3.1-Mb genome of an outbreak methicillin-resistant Staphylococcus aureus (MRSA) strain (TW20) contains evidence of recently acquired DNA, including two large regions (635 kb and 127 kb). The strain is resistant to a wide range of antibiotics, antiseptics, and heavy metals due to resistance genes encoded on mobile ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01255-09

    authors: Holden MT,Lindsay JA,Corton C,Quail MA,Cockfield JD,Pathak S,Batra R,Parkhill J,Bentley SD,Edgeworth JD

    更新日期:2010-02-01 00:00:00

  • ISOLOGOUS INTERFERENCE WITH ULTRAVIOLET AND X-RAY IRRADIATED BACTERIOPHAGE T2.

    abstract::Levy, Stuart B. (Institut du Radium, Paris, France). Isologous interference with ultraviolet and X-ray irradiated bacteriophage T(2). J. Bacteriol. 87:1330-1338. 1964.-Qualitative and quantitative analysis of the interference capacity of an irradiated T(2) bacteriophage was made with ultraviolet and X-ray irradiation....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.6.1330-1338.1964

    authors: LEVY SB

    更新日期:1964-06-01 00:00:00

  • Sorting out bacterial viability with optical tweezers.

    abstract::We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.19.5551-5555.2000

    authors: Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

    更新日期:2000-10-01 00:00:00

  • Cyclic Dinucleotide-Controlled Regulatory Pathways in Streptomyces Species.

    abstract::The cyclic dinucleotides cyclic 3′,5′-diguanylate (c-di-GMP) and cyclic 3′,5′-diadenylate (c-di-AMP) have emerged as key components of bacterial signal transduction networks. These closely related second messengers follow the classical general principles of nucleotide signaling by integrating diverse signals into regu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00423-15

    authors: Tschowri N

    更新日期:2016-01-01 00:00:00

  • Caulobacter crescentus synthesizes an S-layer-editing metalloprotease possessing a domain sharing sequence similarity with its paracrystalline S-layer protein.

    abstract::Strains of Caulobacter crescentus elaborate an S-layer, a two-dimensional protein latticework which covers the cell surface. The S-layer protein (RsaA) is secreted by a type I mechanism (relying on a C-terminal signal) and is unusual among type I secreted proteins because high levels of protein are produced continuous...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.10.2709-2718.2002

    authors: Umelo-Njaka E,Bingle WH,Borchani F,Le KD,Awram P,Blake T,Nomellini JF,Smit J

    更新日期:2002-05-01 00:00:00

  • NONPHOTOREACTIVATING REPAIR OF ULTRAVIOLET LIGHT-DAMAGED MICROCOCCUS LYSODEIKTICUS CELLS.

    abstract::Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged Micrococcus lysodeikticus cells. J. Bacteriol. 89:1225-1230. 1965.-Micrococcus lysodeikticus cells are highly resistant to ultraviolet radiation. The shape of the dose-survival...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.5.1225-1230.1965

    authors: ELDER RL,BEERS RF Jr

    更新日期:1965-05-01 00:00:00

  • A SecE mutation that modulates SecY-SecE translocase assembly, identified as a specific suppressor of SecY defects.

    abstract::The SecY39(Cs) (cold-sensitive) alteration of Arg357 results in a defect of translocation initiation. As a means to dissect the Sec translocation machinery, we isolated mutations that act as suppressors of the secY39 defect. A specific secE mutation, designated secE105, was thus isolated. This mutation proved to be id...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.3.948-956.2003

    authors: Mori H,Akiyama Y,Ito K

    更新日期:2003-02-01 00:00:00