Abstract:
:The initiation process of deoxyribonucleic acid (DNA) replication in Escherichia coli has been studied using the thermoreversible dna initiation mutant E. coli HfrHl65/120/6 dna-252. This dna mutation was incorrectly classed as a dnaA mutation. Biochemical and genetic evidence suggests that the dna-252 mutant is a novel dnaB mutant, possessing phenotypic properties which distinguish it from other dnaB mutants. Sensitivity of reinitiation in the dna-252 mutant to specific inhibitors of protein, ribonucleic acid (RNA), and DNA synthesis was studied. Reinitiation is shown to be sensitive to rifampin and streptolydigin but not to cholramphenicol. Thus, the dna-252 gene product appears to be required during the initiation process for a step occurring either before or during synthesis of an RNA species (origin-RNA). Using reversible inhibition of RNA synthesis by streptolydigin of a streptolydigin-sensitive derivative of the dna-252 mutant, the dna-252 gene product is shown to be directly involved in the synthesis of an orgin-RNA species. These results are included in a schematic model presented in the accompanying paper of the temporal sequence of events occurring during the initiation process.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zyskind JW,Smith DWdoi
10.1128/JB.129.3.1476-1486.1977subject
Has Abstractpub_date
1977-03-01 00:00:00pages
1476-86issue
3eissn
0021-9193issn
1098-5530journal_volume
129pub_type
杂志文章abstract::Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 4...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.1.41-46.1972
更新日期:1972-04-01 00:00:00
abstract::The outer membranes of Escherichia coli K-12 cells carrying a derepressed F-like R factor contain about 7 times 10-4 molecules per cell of a protein similar to the subunit of the sex pili specified by the R factor. This protein pool is absent in cells carrying the repressed variant of the R factor. The size of the poo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.1.59-65.1975
更新日期:1975-04-01 00:00:00
abstract::A deoxyribonucleic acid-directed in vitro system for the synthesis of Escherichia coli carbamoylphosphate synthase has been developed, and its properties have been studied. The system uses the deoxyribonucleic acid of a lambda phage carrying the car genes (lambdadcarAB) as template and mediates the synthesis of both s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.58-66.1980
更新日期:1980-01-01 00:00:00
abstract::Herman, Alberta (University of Wisconsin, Madison) and Harlyn Halvorson. Identification of the structural gene for beta-glucosidase in Saccharomyces lactis. J. Bacteriol. 85:895-900. 1963.-Three allelic forms (B(h, m, l)) of the structural gene for beta-glucosidase have been identified in the yeast Saccharomyces lacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.895-900.1963
更新日期:1963-04-01 00:00:00
abstract::Gas vesicles, isolated from lysed Halobacterium halobium cells, gave an amino acid analysis which accounted for 78% of the weight, and the balance was mainly salt and water. One percent of tightly bound d-galactose was found, as well as 2% of phosphate that was not released by treatment which promotes beta-elimination...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.1058-1067.1973
更新日期:1973-06-01 00:00:00
abstract::We report here the identification of a new lipoprotein, NlpI, in Escherichia coli K-12. The NlpI structural gene (nlpI) is located between the genes pnp (polynucleotide phosphorylase) and deaD (RNA helicase) at 71 min on the E. coli chromosome. The nlpI gene encodes a putative polypeptide of approximately 34 kDa, and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.14.4318-4325.1999
更新日期:1999-07-01 00:00:00
abstract::We recently demonstrated that Pseudomonas aeruginosa PAO1 undergoes a pronounced phenotypic change when introduced into the intestines of rats during surgical injury. Recovered strains displayed a specific phenotype (termed the P2 phenotype) characterized by altered pyocyanin production, high collagenase activity, hig...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01050-13
更新日期:2014-01-01 00:00:00
abstract::In anaerobic cultures of Mucor rouxii, morphogenesis was strongly dependent on hexose concentration as well as pCO(2). At low levels of hexose or CO(2), or both, hyphal development occurred; at high levels, the fungus developed as yeast cells. Other dimorphic strains of Mucor responded similarly to hexose and CO(2) bu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.5.1586-1594.1968
更新日期:1968-11-01 00:00:00
abstract::Drugs known to interrupt the chain of protein synthesis (streptomycin, chloramphenicol, ethidium bromide) appeared to affect production of the group D lysin by Streptococcus zymogenes by inhibiting growth. Drugs which block cell wall synthesis (vancomycin, bacitracin, d-cycloserine, and phosphonomycin) inhibited lytic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.2.844-848.1971
更新日期:1971-11-01 00:00:00
abstract::The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.191-199.1992
更新日期:1992-01-01 00:00:00
abstract::Integrons constitute a novel family of DNA elements which evolved by site-specific integration of discrete units between two conserved segments. On the In4 integron of Tn1696, a precisely inserted gene cassette of 1,549 bp conferring nonenzymatic chloramphenicol resistance (cmlA) is present between the streptomycin-sp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4493-4502.1991
更新日期:1991-07-01 00:00:00
abstract::Thermococcus sp. strain CL1 is a hyperthermophilic, anaerobic, and heterotrophic archaeon isolated from a Paralvinella sp. polychaete worm living on an active deep-sea hydrothermal sulfide chimney on the Cleft Segment of the Juan de Fuca Ridge. To further understand the distinct characteristics of this archaeon at the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01016-12
更新日期:2012-09-01 00:00:00
abstract::Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01563-07
更新日期:2008-04-01 00:00:00
abstract::Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00644-09
更新日期:2009-10-01 00:00:00
abstract::Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.2.446-453.1964
更新日期:1964-02-01 00:00:00
abstract::The plasmid-encoded yop genes of pathogenic yersiniae are regulated by the environmental stimuli calcium and temperature. A novel protein, LcrQ, which exhibits a key function in the negative calcium-controlled pathway, was identified. DNA sequence analysis revealed that LcrQ has a molecular mass of 12,412 daltons and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.10.3355-3363.1992
更新日期:1992-05-01 00:00:00
abstract::The genes for biosynthesis of the branched-chain amino acids leucine, isoleucine, and valine in Lactococcus lactis subsp. lactis NCDO2118 were characterized by cloning, complementation in Escherichia coli and Bacillus subtilis, and nucleotide sequence analysis. Nine structural genes are clustered on a 12-kb DNA fragme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6580-6589.1992
更新日期:1992-10-01 00:00:00
abstract::In the photosynthetic bacterium Rhodobacter capsulatus, the enzyme DNA gyrase has been implicated in the expression of genes for anaerobic metabolic processes such as nitrogen fixation and photosynthesis. To assess the involvement of supercoiling in anaerobic gene expression, we have developed an assay to detect in vi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4836-4843.1989
更新日期:1989-09-01 00:00:00
abstract::The strict human pathogen Neisseria gonorrhoeae is exposed to oxidative damage during infection. N. gonorrhoeae has many defenses that have been demonstrated to counteract oxidative damage. However, recN is the only DNA repair and recombination gene upregulated in response to hydrogen peroxide (H(2)O(2)) by microarray...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00801-06
更新日期:2006-11-01 00:00:00
abstract::Phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Azotobacter vinelandii, like the corresponding enzyme from other organisms, is activated by acetyl coenzyme A and inhibited by l-aspartate. Both modifiers affect primarily the affinity of the enzyme for phosphoenolpyruvate. This is the first enzyme with a strictly ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.1.31-36.1971
更新日期:1971-04-01 00:00:00
abstract::Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospire...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1618-1623.1964
更新日期:1964-12-01 00:00:00
abstract::Escherichia coli WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first few days after plating at densities above 10(8) on plates lacking the required amino acid. They lose viability for some days, and then a subpopulation recovers and there is cell turnover. At very low plating d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2906-2910.1998
更新日期:1998-06-01 00:00:00
abstract::Benzoate catabolism is thought to play a key role in aerobic bacterial degradation of biphenyl and polychlorinated biphenyls (PCBs). Benzoate catabolic genes were cloned from a PCB degrader, Rhodococcus sp. strain RHA1, by using PCR amplification and temporal temperature gradient electrophoresis separation. A nucleoti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.22.6598-6606.2001
更新日期:2001-11-01 00:00:00
abstract::The properties of the anthranilate synthetase complex and its separated subunits were compared in catalyzing the anthranilate synthetase reaction, chorismate + l-glutamine or NH(4) (+) --> anthranilate, and the transferase reaction, anthranilate + 5'-phosphorylribosyl-1-pyrophosphate --> phosphoribosyl anthranilate. I...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.734-742.1969
更新日期:1969-02-01 00:00:00
abstract::The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00426-09
更新日期:2009-08-01 00:00:00
abstract::A transformable strain of Bacillus subtilis 6160, a derivative of B. subtilis 168, produces three kinds of casein hydrolytic enzymes (alkaline protease, neutral protease, and esterase) in a culture medium. B. natto IAM 1212 produces 15 to 20 times as much total proteolytic activity as does B. subtilis. Extracellular p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.1.82-91.1974
更新日期:1974-07-01 00:00:00
abstract::In Cyanophora paradoxa, the allophycocyanin apoprotein subunits, alpha and beta, are encoded in the cyanelle (plastid) genome. These genes were transferred to the cyanobacterium Synechococcus sp. PCC 7002 on a plasmid replicon. Phycobilisomes isolated from transformed cyanobacteria were found to contain C. paradoxa al...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.1830-1835.1987
更新日期:1987-05-01 00:00:00
abstract::The stationary phase/general stress response sigma factor RpoS (σ(S)) is necessary for adaptation and restoration of homeostasis in stationary phase. As a physiological consequence, its levels are tightly regulated at least at two levels. Multiple small regulatory RNA molecules modulate its translation, in a manner th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01013-13
更新日期:2014-02-01 00:00:00
abstract::Hampar, Berge (National Institute of Dental Research, Bethesda, Md.). Persistent cyclic herpes simplex virus infection in vitro. IV. Changes in the severity of the infections in the presence of antibody. J. Bacteriol. 92:1741-1747. 1966.-The severity of persistent herpes simplex virus (HSV) infections maintained in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.6.1741-1747.1966
更新日期:1966-12-01 00:00:00
abstract::The Escherichia coli K42 capsular polysaccharide consists of leads to 3)-alpha-D-Galp-(1 leads to 3)-alpha-D-GalUAp-(1 leads to 3)-alpha-L-Fucp-(1 leads to repeating units. The E. coli K42 and Klebsiella K63 antigens are serologically identical. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.390-391.1978
更新日期:1978-01-01 00:00:00