Anthranilate synthetase, an enzyme specified by the tryptophan operon of Escherichia coli: Comparative studies on the complex and the subunits.

abstract：:A DNA region carrying lysS, the gene encoding the lysyl-tRNA synthetase, was cloned from the extreme thermophile prokaryote Thermus thermophilus VK-1 and sequenced. The analysis indicated an open reading frame encoding a protein of 492 amino acids. This putative protein has significant homologies to previously sequenc...

journal_title：Journal of bacteriology

authors： Chen J,Brevet A,Lapadat-Tapolsky M,Blanquet S,Plateau P

更新日期：1994-05-01 00:00:00

abstract：:The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...

journal_title：Journal of bacteriology

authors： Bochar DA,Brown JR,Doolittle WF,Klenk HP,Lam W,Schenk ME,Stauffacher CV,Rodwell VW

更新日期：1997-06-01 00:00:00

abstract：:Alysiella bovis adheres to surfaces by means of short, ruthenium red-staining, rod-like fimbriae. The fimbriae remain associated with the cell envelope of A. bovis, even when sonicated or exposed sequentially to toluene, Triton X-100, lysozyme, ribonuclease, and deoxyribonuclease. Adhesion of outer membrane-derived ce...

journal_title：Journal of bacteriology

authors： Irvin RT,To M,Costerton JW

更新日期：1984-11-01 00:00:00

abstract：:Helicobacter pylori urease requires nickel ions in the enzyme active site for catalytic activity. Nickel ions must, therefore, be actively acquired by the bacterium. NixA (high-affinity nickel transport protein)-deficient mutants of H. pylori retain significant urease activity, suggesting the presence of alternate nic...

journal_title：Journal of bacteriology

authors： Hendricks JK,Mobley HL

更新日期：1997-09-01 00:00:00

abstract：:The fate of 35-S during anaerobic metabolism of [35-S]sulfate, [35-S]thiosulfate, and [35-S]sulfate plus unlabeled thiosulfate by washed cell suspensions of Desulfovibrio spp, and of [35-S]thiosulfate by growing D. desulfuricans was examined. The results appear to be inconsistent with the hypothesis that thiosulfate i...

journal_title：Journal of bacteriology

authors： Chambers LA,Trudinger PA

更新日期：1975-07-01 00:00:00

abstract：:The transcriptional regulatory protein Rgg coordinates amino acid catabolism and virulence factor expression in Streptococcus pyogenes. We used a proteomic approach to compare cytoplasmic proteins isolated from S. pyogenes wild-type strain NZ131 (serotype M49) to proteins isolated from an rgg mutant strain during the ...

journal_title：Journal of bacteriology

authors： Chaussee MA,Callegari EA,Chaussee MS

更新日期：2004-11-01 00:00:00

abstract：:The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the bi...

journal_title：Journal of bacteriology

authors： Pudlik AM,Lolkema JS

更新日期：2012-07-01 00:00:00

abstract：:Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmi...

journal_title：Journal of bacteriology

authors： Lopez-Alvarez J,Gyles CL,Shipley PL,Falkow S

更新日期：1980-02-01 00:00:00

abstract：:Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to the consumption of raw seafood. It secretes a metalloprotease that is associated with skin lesions and serious hemorrhagic complications. In this study, we purified and characterized an extracellular metalloprotease (designated ...

journal_title：Journal of bacteriology

authors： Chang AK,Kim HY,Park JE,Acharya P,Park IS,Yoon SM,You HJ,Hahm KS,Park JK,Lee JS

更新日期：2005-10-01 00:00:00

abstract：:Bacitracin is a peptide antibiotic nonribosomally produced by Bacillus licheniformis. The bcrABC genes which confer bacitracin resistance to the bacitracin producer encode ATP binding cassette (ABC) transporter proteins, which are hypothesized to pump out bacitracin from the cells. Bacillus subtilis 168, which has no ...

journal_title：Journal of bacteriology

authors： Ohki R,Tateno K,Okada Y,Okajima H,Asai K,Sadaie Y,Murata M,Aiso T

更新日期：2003-01-01 00:00:00

abstract：:Campylobacter jejuni commensally colonizes the cecum of birds. The RacR (reduced ability to colonize) response regulator was previously shown to be important in avian colonization. To explore the means by which RacR and its cognate sensor kinase RacS may modulate C. jejuni physiology and colonization, ΔracR and ΔracS ...

journal_title：Journal of bacteriology

authors： Apel D,Ellermeier J,Pryjma M,Dirita VJ,Gaynor EC

更新日期：2012-05-01 00:00:00

abstract：:There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...

journal_title：Journal of bacteriology

authors： Gibson JL,Tabita FR

更新日期：1985-12-01 00:00:00

abstract：:Spectinomycin-resistant (Spcr) mutants of Escherichia coli were isolated from nutrient agar plates containing 20% sucrose and 100 mug of spectinomycin per ml. About one-third of the Spcr mutants thus obtained were sucrose dependent (Sucd) and were classified into two types: I, those unable to grow on sucrose-free medi...

journal_title：Journal of bacteriology

authors： Miyoshi Y,Yamagata H

更新日期：1976-01-01 00:00:00

abstract：UNLABELLED:During biosynthesis of [NiFe]-hydrogenase 2 (Hyd-2) of Escherichia coli, a 15-amino-acid C-terminal peptide is cleaved from the catalytic large subunit precursor, pro-HybC. This peptide is removed only after NiFe(CN)2CO cofactor insertion by the Hyp accessory protein machinery has been completed, suggesting ...

journal_title：Journal of bacteriology

authors： Thomas C,Muhr E,Sawers RG

更新日期：2015-09-01 00:00:00

abstract：:Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...

journal_title：Journal of bacteriology

authors： Aronson AI,Bell C,Fulroth B

更新日期：2005-05-01 00:00:00

abstract：:The buoyant density in rapidly growing Streptococcus faecium 9790 cells varies over the cell cycle, in contrast to the density in Escherichia coli. Buoyant density in S. faecium was measured by using Percoll (Pharmacia Fine Chemicals, Piscataway, N.J.) density gradients. We found that the mean and coefficient of varia...

journal_title：Journal of bacteriology

authors： Glaser D,Higgins M

更新日期：1989-02-01 00:00:00

abstract：:The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...

journal_title：Journal of bacteriology

authors： Peng JB,Yan WM,Bao XZ

更新日期：1994-05-01 00:00:00

abstract：:A genetic approach was used to establish the route of UMP biosynthesis in Sulfolobus acidocaldarius, a member of the hyperthermophilic division (the Crenarchaeota) of the Archaea domain. Pyrimidine auxotrophs of S. acidocaldarius DG6 were isolated by direct selection and by brute-force methods. Enzymatic assay of extr...

journal_title：Journal of bacteriology

authors： Grogan DW,Gunsalus RP

更新日期：1993-03-01 00:00:00

abstract：:Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling...

journal_title：Journal of bacteriology

authors： Karr DB,Emerich DW

更新日期：1989-06-01 00:00:00

abstract：:SigB is an alternative sigma factor that controls a large regulon in Staphylococcus aureus. Activation of SigB requires RsbU, a protein phosphatase 2C (PP2C)-type phosphatase. In a closely related organism, Bacillus subtilis, RsbU activity is stimulated upon interaction with RsbT, a kinase, which following an activati...

journal_title：Journal of bacteriology

authors： Pané-Farré J,Jonas B,Hardwick SW,Gronau K,Lewis RJ,Hecker M,Engelmann S

更新日期：2009-04-01 00:00:00

abstract：:Polysaccharide-containing material was located by histochemical staining on ultrathin sections of Bacillus cereus and several clostridial species during various phases of growth. These components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. In B. cere...

journal_title：Journal of bacteriology

authors： Walker PD,Short J

更新日期：1969-06-01 00:00:00

abstract：:Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fr...

journal_title：Journal of bacteriology

authors： Hou CT,Lillard MO

更新日期：1976-04-01 00:00:00

abstract：:The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various concentrations of oxygen or types of growth substrates. Spectrophotometric analysis revealed that the quantity and type...

journal_title：Journal of bacteriology

authors： Ensley BD Jr,Finnerty WR

更新日期：1980-06-01 00:00:00

abstract：:Haemophilus parasuis is a swine pathogen of significant industry concern, but little is known about how the organism causes disease. A related human pathogen, Haemophilus influenzae, has been better studied, and many of its virulence factors have been identified. Two of these, outer membrane proteins P2 and P5, are kn...

journal_title：Journal of bacteriology

authors： Mullins MA,Register KB,Bayles DO,Loving CL,Nicholson TL,Brockmeier SL,Dyer DW,Phillips GJ

更新日期：2009-10-01 00:00:00

abstract：:Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of...

journal_title：Journal of bacteriology

authors： Madden MS,Paustian TD,Ludden PW,Shah VK

更新日期：1991-09-01 00:00:00

abstract：:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not a...

journal_title：Journal of bacteriology

authors： Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms N

更新日期：1995-01-01 00:00:00

abstract：:A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (ten...

journal_title：Journal of bacteriology

authors： Bradshaw HD Jr,Traxler BA,Minkley EG Jr,Nester EW,Gordon MP

更新日期：1990-07-01 00:00:00

abstract：:We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repe...

journal_title：Journal of bacteriology

authors： Kato A,Tanabe H,Utsumi R

更新日期：1999-09-01 00:00:00

abstract：:Mycelial mats of Ascodesmis sphaerospora were fixed and embedded for electron microscopy, and thin sections of 1-mm blocks, taken from the 1st to the 7th mm behind the hyphal tips, were cut parallel to the long axis of the hyphae. The hyphal tip region is characterized by an outer zone of electron-transparent vesicles...

journal_title：Journal of bacteriology

authors： Brenner DM,Carroll GC

更新日期：1968-02-01 00:00:00

abstract：:The electron transport system of Neisseria gonorrhoeae was partially characterized by using spectrophotometric, spectroscopic, and oxygen consumption measurements. The effects of selected electron transport inhibitors (amytal, rotenone, 2-heptyl-4-hydroxyquinoline, antimycin A1, and potassium cyanide [KCN]) on electro...

journal_title：Journal of bacteriology

authors： Kenimer EA,Lapp DF

更新日期：1978-05-01 00:00:00