Abstract:
:Helicobacter pylori urease requires nickel ions in the enzyme active site for catalytic activity. Nickel ions must, therefore, be actively acquired by the bacterium. NixA (high-affinity nickel transport protein)-deficient mutants of H. pylori retain significant urease activity, suggesting the presence of alternate nickel transporters. Analysis of the nucleotide sequence of the H. pylori genome revealed a homolog of NikD, a component of an ATP-dependent nickel transport system in Escherichia coli. Based on this sequence, a 378-bp DNA fragment was PCR amplified from H. pylori genomic DNA and used as a probe to identify an H. pylori lambda ZAPII genomic library clone that carried these sequences. Four open reading frames of 621, 273, 984, and 642 bp (abcABCD) were revealed by sequencing and predicted polypeptides of 22.7, 9.9, 36.6, and 22.8 kDa, respectively. The 36.6-kDa polypeptide (AbcC) has significant homology (56% amino acid sequence identity) to an E. coli ATP-binding protein component of an ABC transport system, while none of the other putative proteins are significantly homologous to polypeptides in the available databases. To determine the possible contribution of these genes to urease activity, abcC and abcD were each insertionally inactivated with a kanamycin resistance (aphA) cassette and allelic exchange mutants of each gene were constructed in H. pylori UMAB41. Mutation of abcD resulted in an 88% decrease in urease activity to 27 +/- 31 mumol of NH3/min/mg of protein (P < 0.0001), and a double mutant of nixA and abcC resulted in the near abolishment of urease activity (1.1 +/- 1.4 mumol of NH3/min/mg of protein in the double mutant versus 228 +/- 92 mumol of NH3/min/mg of protein in the parent [P < 0.0001]). Synthesis of urease apoenzyme, however, was unaffected by mutations in any of the abc genes. We conclude that the abc gene cluster, in addition to nixA, is involved in production of a catalytically active urease.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hendricks JK,Mobley HLdoi
10.1128/jb.179.18.5892-5902.1997subject
Has Abstractpub_date
1997-09-01 00:00:00pages
5892-902issue
18eissn
0021-9193issn
1098-5530journal_volume
179pub_type
杂志文章abstract::Spontaneous mutators of Salmonella typhimurium LT2 were generated by inserting the transposable element Tn5 or Tn10 into the bacterial chromosome. Two mutators mapped at the position of the mutH and mutL loci of S. typhimurium, and two other mutators mapped at positions corresponding to the mutS and uvrD loci of Esche...
journal_title:Journal of bacteriology
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doi:10.1128/JB.147.3.827-835.1981
更新日期:1981-09-01 00:00:00
abstract::Wild-type Escherichia coli cannot grow on L-1,2-propanediol; mutants that can do so have increased basal activity of an NAD-linked L-1,2-propanediol oxidoreductase. This enzyme belongs to the L-fucose system and functions normally as L-lactaldehyde reductase during fermentation of the methylpentose. In wild-type cells...
journal_title:Journal of bacteriology
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doi:10.1128/JB.159.2.725-729.1984
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journal_title:Journal of bacteriology
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doi:10.1128/jb.179.16.5232-5237.1997
更新日期:1997-08-01 00:00:00
abstract::The symbiotic plasmid of Rhizobium etli CE3 belongs to the RepABC family of plasmid replicons. This family is characterized by the presence of three conserved genes, repA, repB, and repC, encoded by the same DNA strand. A long intergenic sequence (igs) between repB and repC is also conserved in all members of the plas...
journal_title:Journal of bacteriology
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doi:10.1128/jb.182.11.3117-3124.2000
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abstract::The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.778-784.2005
更新日期:2005-01-01 00:00:00
abstract::Random transposon Tn5 mutagenesis of Bradyrhizobium sp. (Arachis) strain NC92, a member of the cowpea cross-inoculation group, was carried out, and kanamycin-resistant transconjugants were tested for their symbiotic phenotype on three host plants: groundnut, siratro, and pigeonpea. Two nodulation (Nod- phenotype) muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2177-2186.1987
更新日期:1987-05-01 00:00:00
abstract::Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.840-843.1974
更新日期:1974-09-01 00:00:00
abstract::Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01259-10
更新日期:2011-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.669-673.1989
更新日期:1989-02-01 00:00:00
abstract::Using a number of approaches we were unable to demonstrate a chemotactic response of Myxococcus xanthus to a variety of defined and complex materials. These data in addition to a number of prima facie arguments considerably reduce the likelihood that M. xanthus possesses a mechanism for chemotactic behavior. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.437-442.1983
更新日期:1983-04-01 00:00:00
abstract::The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.2.361-364.1970
更新日期:1970-02-01 00:00:00
abstract::We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01415-09
更新日期:2010-03-01 00:00:00
abstract::The distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species was examined. Enzyme activity was demonstrated in cell-free growth media from all members of the family Vibrionaceae which were tested except Plesiomonas shigelloides. In each case, enzyme was produced in exponential to e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.132-136.1979
更新日期:1979-07-01 00:00:00
abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01619-06
更新日期:2007-04-01 00:00:00
abstract::Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S. salivarius fimbriae. Fimbriae were extracted by shearing the cell surface of hyperfimbriated mutant A37 (a spontaneous m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.9.2724-2732.2001
更新日期:2001-05-01 00:00:00
abstract::In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.375-382.1972
更新日期:1972-08-01 00:00:00
abstract::A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1366-1379.1971
更新日期:1971-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.8.2993-3001.2006
更新日期:2006-04-01 00:00:00
abstract::Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.755-760.1983
更新日期:1983-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.16.4866-4875.2001
更新日期:2001-08-01 00:00:00
abstract::Various methods of inducing autolysis of Escherichia coli cells were investigated, some being described here for the first time. For the autolysis of growing cells only induction methods interfering with the biosynthesis of peptidoglycan were taken into consideration, whereas with harvested cells autolysis was induced...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::omega 4403 is the site of a Tn5 lac insertion in the Myxococcus xanthus genome that fuses lacZ expression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C-signaling, are required for expression of Tn5 lac omega 4403. We have cloned DNA upstream of the omega 440...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.9.2539-2550.1996
更新日期:1996-05-01 00:00:00
abstract::Cystic fibrosis (CF) patients chronically infected with both Pseudomonas aeruginosa and Staphylococcus aureus have worse health outcomes than patients who are monoinfected with either P. aeruginosa or S. aureus We showed previously that mucoid strains of P. aeruginosa can coexist with S. aureusin vitro due to the tran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00559-19
更新日期:2020-03-26 00:00:00
abstract::The Bacillus subtilis dciA operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4605-4614.1993
更新日期:1993-08-01 00:00:00
abstract::The nucleotide sequence of a 2220-base-pair fragment containing the btuB gene of Escherichia coli was determined. There was a single open reading frame which was translated into a 614-amino-acid polypeptide, the first 20 amino acids of which comprised a typical leader sequence. The putative mature or processed form ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.3.904-908.1985
更新日期:1985-03-01 00:00:00
abstract::Oxalurate, the gratuitous inducer of the allantoin degradative enzymes, was taken into the cell by an energy-dependent active transport system with an apparent Km of 1.2 mM. Efflux of previously accumulated oxalurate was rapid, with a half-life of about 2 min. The oxalurate uptake system appears to be both constitutiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.917-923.1979
更新日期:1979-09-01 00:00:00
abstract::A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.468-470.1988
更新日期:1988-01-01 00:00:00
abstract::Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.723-728.1982
更新日期:1982-08-01 00:00:00
abstract::Previously we have described a mutant Hfr strain in which incompatibility between the integrated F factor and an autonomous F-prime (F') factor was abolished. The mutation (inc) was located in the integrated F factor. F-prime factors isolated from the mutant Hfr strain have the same incompatibility behavior as those i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.348-353.1976
更新日期:1976-07-01 00:00:00
abstract::The PriA protein, a component of the phiX174-type primosome, was previously shown to be essential for damage-inducible DNA replication in Escherichia coli, termed inducible stable DNA replication. Here, we show that priA::kan null mutants are defective in transductional and conjugational homologous recombination and a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.5.1258-1264.1996
更新日期:1996-03-01 00:00:00