Mutants of Salmonella typhimurium that are insensitive to catabolite repression of proline degradation.

Abstract:

:In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes have been isolated by selection on agar plates containing glucose and proline. The specificity of the catabolite repression-insensitive character for the enzymes of proline utilization has been confirmed by an analysis of other unrelated catabolic enzymes. Histidase and amylomaltase of the mutant strains are equally as sensitive to glucose repression as are the enzymes from the wild type. All four PutR mutants exhibit higher induced and higher basal levels of proline oxidase as compared with the corresponding wild-type levels. The mutations of three strains tested are cotransducible with constitutive, pleiotrophic-negative and structural gene mutations of the put region. Three-factor crosses indicate that two putR mutations are located at one end of the cluster of put mutations.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Newell SL,Brill WJ

doi

10.1128/JB.111.2.375-382.1972

subject

Has Abstract

pub_date

1972-08-01 00:00:00

pages

375-82

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

111

pub_type

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