Abstract:
:The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis consensus sequences. Extracts from E. coli cells, containing the sacA gene, displayed a sucrose-hydrolyzing activity. However, no transfructosylation activity (exchange reaction or levan formation) could be detected. This sucrase activity was different from that observed with the purified extracellular protein B46 from Z. mobilis. These two proteins showed different electrophoretic mobilities and molecular masses and shared no immunological similarity. Thus, the product of sacA (a polypeptide of 58.4-kDa molecular mass) is a new sucrase from Z. mobilis. The amino acid sequence, deduced from the nucleotide sequence of sacA, showed strong homologies with the sucrases from Bacillus subtilis, Salmonella typhimurium, and Vibrio alginolyticus.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gunasekaran P,Karunakaran T,Cami B,Mukundan AG,Preziosi L,Baratti Jdoi
10.1128/jb.172.12.6727-6735.1990subject
Has Abstractpub_date
1990-12-01 00:00:00pages
6727-35issue
12eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00853-06
更新日期:2006-12-01 00:00:00
abstract::Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.3.1204-1213.1983
更新日期:1983-12-01 00:00:00
abstract::Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (K(m) of 1 muM and V(max) of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (K(m) of 10 muM for l-alanine and 20 muM for d-alanine a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.3.1085-1092.1974
更新日期:1974-12-01 00:00:00
abstract::Hampar, Berge (National Institute of Dental Research, Bethesda, Md.). Persistent cyclic herpes simplex virus infection in vitro. IV. Changes in the severity of the infections in the presence of antibody. J. Bacteriol. 92:1741-1747. 1966.-The severity of persistent herpes simplex virus (HSV) infections maintained in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.6.1741-1747.1966
更新日期:1966-12-01 00:00:00
abstract::The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.6.2148-2150.2001
更新日期:2001-03-01 00:00:00
abstract::Ramakrishnan, T. (Yale University, New Haven, Conn.), and Edward A. Adelberg. Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J. Bacteriol. 89:654-660. 1965.-A tightly clustered set of five structural genes governs the synthesis of the five enzymes of isole...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.3.654-660.1965
更新日期:1965-03-01 00:00:00
abstract::Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously showed that some M. leprae pseudogenes are transcribed at high levels and that their expression levels change following infe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00120-09
更新日期:2009-05-01 00:00:00
abstract::Pseudomonas sp. strain ACP is capable of growth on 1-aminocyclopropane-1-carboxylate (ACC) as a nitrogen source owing to induction of the enzyme ACC deaminase and the subsequent conversion of ACC to alpha-ketobutyrate and ammonia (M. Honma, Agric. Biol. Chem. 49:567-571, 1985). The complete amino acid sequence of puri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5260-5265.1991
更新日期:1991-09-01 00:00:00
abstract::Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.14.3578-3583.1998
更新日期:1998-07-01 00:00:00
abstract::Nishida, Shoki (Kanazawa University, Kanazawa, Japan), and Gizo Nakagawara. Relationship between toxigenicity and sporulating potency of Clostridium novyi. J. Bacteriol. 89:993-995. 1965.-The less toxigenic the strains, the stronger was the sporulating potency of Clostridium novyi strains isolated. This was confirmed ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.993-995.1965
更新日期:1965-04-01 00:00:00
abstract::Vibrio cholerae is a motile bacterium responsible for the disease cholera, and motility has been hypothesized to be inversely regulated with virulence. We examined the transcription profiles of V. cholerae strains containing mutations in flagellar regulatory genes (rpoN, flrA, flrC, and fliA) by utilizing whole-genome...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00949-09
更新日期:2009-11-01 00:00:00
abstract::Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1700-1704.1964
更新日期:1964-12-01 00:00:00
abstract::Pseudomonas stutzeri A1501 is a versatile nitrogen-fixing bacterium capable of living in diverse environments and coping with various oxidative stresses. NfiS, a regulatory noncoding RNA (ncRNA) involved in the control of nitrogen fixation in A1501, was previously shown to be required for optimal resistance to H2O2; h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00334-19
更新日期:2019-09-06 00:00:00
abstract::Unique lipids found in Azotobacter vinelandii cysts are derived primarily from beta-hydroxybutyrate used to induce encystment. Tracer studies with beta-[14C]hydroxybutyrate showed that the biosynthesis of these compounds during encystment began at 8 to 12 h after induction and reached maximal levels after 2 days, Seve...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.1.91-96.1981
更新日期:1981-07-01 00:00:00
abstract::The Neurospora crassa assimilatory nitrite reductase structural gene, nit-6, has been isolated. A cDNA library was constructed from poly(A)+ RNA isolated from Neurospora mycelia in which nitrate assimilation had been induced. This cDNA was ligated into lambda ZAP II (Stratagene) and amplified. This library was then sc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2379-2392.1993
更新日期:1993-04-01 00:00:00
abstract:UNLABELLED:FimV is a Pseudomonas aeruginosa inner membrane protein that regulates intracellular cyclic AMP (cAMP) levels-and thus type IV pilus (T4P)-mediated twitching motility and type II secretion (T2S)-by activating the adenylate cyclase CyaB. Its cytoplasmic domain contains three predicted tetratricopeptide repeat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00322-16
更新日期:2016-07-28 00:00:00
abstract::The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.191-199.1992
更新日期:1992-01-01 00:00:00
abstract::Candida albicans produces large amounts of the acyclic pentitol D-arabitol in culture and in infected animals and humans, and most strains also grow on minimal D-arabitol medium. An earlier study showed that the major metabolic precursor of D-arabitol in C. albicans was D-ribulose-5-PO4 from the pentose pathway, that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.2971-2976.1995
更新日期:1995-06-01 00:00:00
abstract::Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight ext...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.353-357.1966
更新日期:1966-08-01 00:00:00
abstract::Dominance tests in diploids have confirmed that the product of the tyrR gene is involved in a negative control system affecting the synthesis of both 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and DAHP synthetase (phe). Some tyrR mutants are derepressed for the synthesis of both DAHP synthe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.1145-1150.1973
更新日期:1973-09-01 00:00:00
abstract::Growth of Streptococcus faecalis in the presence of l-malate resulted in the induction of a "malic enzyme" [l-malate:nicotinamide adenine dinucleotide (NAD) oxidoreductase (decarboxylating), E.C. 1.1.1.39]. Synthesis of the malic enzyme did not appear to be subject to catabolite repression by intermediate products of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.705-711.1969
更新日期:1969-05-01 00:00:00
abstract::We report the draft genome of the strain Lactobacillus gigeriorum CRBIP 24.85(T), isolated from a chicken crop. The total length of the 60 scaffolds is about 1.9 Mb, with a GC content of 38% and 2,062 protein-coding sequences (CDS). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01427-12
更新日期:2012-11-01 00:00:00
abstract::Pseudomonas sp. strain LB400 grows on biphenyl as the sole carbon and energy source. This organism also cooxidizes several chlorinated biphenyl congeners. Biphenyl dioxygenase activity in cell extract required addition of NAD(P)H as an electron donor for the conversion of biphenyl to cis-2,3-dihydroxy-2,3-dihydrobiphe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.2.395-400.1993
更新日期:1993-01-01 00:00:00
abstract::Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.512-522.1986
更新日期:1986-11-01 00:00:00
abstract::We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.10.3164-3170.1997
更新日期:1997-05-01 00:00:00
abstract::Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.4.925-930.1966
更新日期:1966-10-01 00:00:00
abstract::The secondary structures of the eubacterial RNase P RNAs are being elucidated by a phylogenetic comparative approach. Sequences of genes encoding RNase P RNA from each of the recognized subgroups (alpha, beta, gamma, and delta) of the proteobacteria have now been determined. These sequences allow the refinement, to ne...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3855-3863.1991
更新日期:1991-06-01 00:00:00
abstract::A macrorestriction map representing the complete physical map of the Rhodobacter sphaeroides 2.4.1 chromosomes has been constructed by ordering the chromosomal DNA fragments from total genomic DNA digested with the restriction endonucleases AseI, SpeI, DraI, and SnaBI. Junction fragments and multiple restriction endon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.5850-5859.1989
更新日期:1989-11-01 00:00:00
abstract::In this study, the H303A mutant form of the cbb(3) oxidase (H303A oxidase), which has the H303A mutation in its catalytic subunit (CcoN), was purified from Rhodobacter sphaeroides. The H303A oxidase showed the same catalytic activity as did the wild-type form of the oxidase (WT oxidase). The heme contents of the mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00443-07
更新日期:2007-08-01 00:00:00
abstract::Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05851-11
更新日期:2011-10-01 00:00:00