Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis.

Abstract:

:The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis consensus sequences. Extracts from E. coli cells, containing the sacA gene, displayed a sucrose-hydrolyzing activity. However, no transfructosylation activity (exchange reaction or levan formation) could be detected. This sucrase activity was different from that observed with the purified extracellular protein B46 from Z. mobilis. These two proteins showed different electrophoretic mobilities and molecular masses and shared no immunological similarity. Thus, the product of sacA (a polypeptide of 58.4-kDa molecular mass) is a new sucrase from Z. mobilis. The amino acid sequence, deduced from the nucleotide sequence of sacA, showed strong homologies with the sucrases from Bacillus subtilis, Salmonella typhimurium, and Vibrio alginolyticus.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gunasekaran P,Karunakaran T,Cami B,Mukundan AG,Preziosi L,Baratti J

doi

10.1128/jb.172.12.6727-6735.1990

subject

Has Abstract

pub_date

1990-12-01 00:00:00

pages

6727-35

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

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