Effects of Dimethylsulfoxide on the Lactose Operon in Escherichia coli.

Abstract:

:Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight extent, and does not affect the differential rate of synthesis of beta-galactosidase. Resting cells remain viable after shaking in the presence of 20% DMSO for 3 hr at 37 C. Both beta-galactosidase and thiogalactoside transacetylase retain almost all activity after incubation in even higher concentrations of the solvent for many hours. DMSO decreases the permeability barrier. The rate of hydrolysis of o-nitrophenyl-beta-d-galactoside (ONPG) in whole cells containing beta-galactosidase but lacking permease is increased in cells treated with 5% DMSO. Several permeaseless strains preinduced for beta-galactosidase will grow on lactose in the presence, but not in the absence, of 5% DMSO. When permeaseless strains are grown on tetrazolium-lactose-agar, the presence of 5% DMSO causes a definite but not marked shift toward the lactose-positive character.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Fowler AV,Zabin I

doi

10.1128/JB.92.2.353-357.1966

keywords:

subject

Has Abstract

pub_date

1966-08-01 00:00:00

pages

353-7

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

92

pub_type

杂志文章
  • Purification and characterization of specific 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase from Escherichia coli B.

    abstract::A phosphatase specific for the hydrolysis of 3-deoxy-d-manno-octulosonate (KDO)-8-phosphate was purified approximately 400-fold from crude extracts of Escherichia coli B. The hydrolysis of KDO-8-phosphate to KDO and inorganic phosphate in crude extracts of E. coli B, grown in phosphate-containing minimal medium, could...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.1.60-68.1980

    authors: Ray PH,Benedict CD

    更新日期:1980-04-01 00:00:00

  • Characterization of a human serum inhibitor of Clostridium histolyticum proteinase(s).

    abstract::Normal animal sera inhibit at least one Clostridium histolyticum proteinase. An assay procedure based on immune hemolysis was developed for the estimation of this inhibition. This inhibitory activity occurs in various levels in the sera of different animal species. The highest titers have been obtained with rat sera. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.6.1969-1976.1968

    authors: Luzzati A,Goldlust MB,Levine L

    更新日期:1968-12-01 00:00:00

  • Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403.

    abstract::Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.11.3762-3778.2005

    authors: Sperandio B,Polard P,Ehrlich DS,Renault P,Guédon E

    更新日期:2005-06-01 00:00:00

  • Genome of Streptococcus oralis strain Uo5.

    abstract::Streptococcus oralis, a commensal species of the human oral cavity, belongs to the Mitis group of streptococci, which includes one of the major human pathogens as well, S. pneumoniae. We report here the first complete genome sequence of this species. S. oralis Uo5, a high-level penicillin- and multiple-antibiotic-resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00321-11

    authors: Reichmann P,Nuhn M,Denapaite D,Brückner R,Henrich B,Maurer P,Rieger M,Klages S,Reinhard R,Hakenbeck R

    更新日期:2011-06-01 00:00:00

  • Alcohol dehydrogenases from a facultative methylotrophic bacterium.

    abstract::Alcohol-oxidizing enzymes of the facultative methylotroph PAR were investigated after growth of the bacteria on methanol and ethanol. During methanol growth only a phenazine methosulfate-linked alcohol dehydrogenase was detected. This enzyme had broad specificity for primary alcohols and was also capable of oxidation ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.1.251-258.1978

    authors: Bellion E,Wu GT

    更新日期:1978-07-01 00:00:00

  • Fibronectin binding to the Salmonella enterica serotype Typhimurium ShdA autotransporter protein is inhibited by a monoclonal antibody recognizing the A3 repeat.

    abstract::ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.15.4931-4939.2004

    authors: Kingsley RA,Abi Ghanem D,Puebla-Osorio N,Keestra AM,Berghman L,Bäumler AJ

    更新日期:2004-08-01 00:00:00

  • Eikenella corrodens phase variation involves a posttranslational event in pilus formation.

    abstract::The human pathogen Eikenella corrodens synthesizes type IV pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. On solid medium, piliated variants form small (S-phase), corroding colonies whereas nonpiliated variants form large (L-phase), noncorroding colonie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.14.4154-4160.1999

    authors: Villar MT,Helber JT,Hood B,Schaefer MR,Hirschberg RL

    更新日期:1999-07-01 00:00:00

  • Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase.

    abstract::The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.802-807.1990

    authors: Pradel E,Marck C,Boquet PL

    更新日期:1990-02-01 00:00:00

  • Cell division in Escherichia coli: role of FtsL domains in septal localization, function, and oligomerization.

    abstract::In Escherichia coli, nine essential cell division proteins are known to localize to the division septum. FtsL is a 13-kDa bitopic membrane protein with a short cytoplasmic N-terminal domain, a membrane-spanning segment, and a periplasmic domain that has a repeated heptad motif characteristic of leucine zippers. Here, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.1.116-129.2000

    authors: Ghigo JM,Beckwith J

    更新日期:2000-01-01 00:00:00

  • uvrC gene function in excision repair in toluene-treated Escherichia coli.

    abstract::We have examined the role of the uvrC gene in UV excision repair by studying incision, excision, repair synthesis, and DNA strand reformation in Escherichia coli mutants made permeable to nucleoside triphosphates by toluene treatment. After irradiation, incisions occur normally in uvrC cells in the presence of nicotin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.397-408.1979

    authors: Sharma S,Moses RE

    更新日期:1979-01-01 00:00:00

  • Iron-regulated surface determinant (Isd) proteins of Staphylococcus lugdunensis.

    abstract::Staphylococcus lugdunensis is the only coagulase-negative Staphylococcus species with a locus encoding iron-regulated surface determinant (Isd) proteins. In Staphylococcus aureus, the Isd proteins capture heme from hemoglobin and transfer it across the wall to a membrane-bound transporter, which delivers it into the c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01195-12

    authors: Zapotoczna M,Heilbronner S,Speziale P,Foster TJ

    更新日期:2012-12-01 00:00:00

  • Yield coefficients of Thiobacillus neapolitanus in continuous culture.

    abstract::Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.3.874-878.1967

    authors: Hempfling WP,Vishniac W

    更新日期:1967-03-01 00:00:00

  • Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

    abstract::A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.3.1072-1074.1974

    authors: Lederberg EM,Cohen SN

    更新日期:1974-09-01 00:00:00

  • Characterization of the Synechocystis strain PCC 6803 penicillin-binding proteins and cytokinetic proteins FtsQ and FtsW and their network of interactions with ZipN.

    abstract::Because very little is known about cell division in noncylindrical bacteria and cyanobacteria, we investigated 10 putative cytokinetic proteins in the unicellular spherical cyanobacterium Synechocystis strain PCC 6803. Concerning the eight penicillin-binding proteins (PBPs), which define three classes, we found that S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00620-09

    authors: Marbouty M,Mazouni K,Saguez C,Cassier-Chauvat C,Chauvat F

    更新日期:2009-08-01 00:00:00

  • The Hfq homolog in Legionella pneumophila demonstrates regulation by LetA and RpoS and interacts with the global regulator CsrA.

    abstract::A gene in Legionella pneumophila that has significant homology to published hfq genes demonstrated regulation by RpoS and the transcriptional regulator LetA. Additionally, Hfq has a positive effect on the presence of transcripts of the genes for CsrA and the ferric uptake regulator Fur. Mutants lacking hfq demonstrate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.4.1527-1532.2005

    authors: McNealy TL,Forsbach-Birk V,Shi C,Marre R

    更新日期:2005-02-01 00:00:00

  • Fractionation and characterization of the phosphoenolpyruvate: fructose 1-phosphotransferase system from Pseudomonas aeruginosa.

    abstract::The initial reactions involved in the catabolism of fructose in Pseudomonas aeruginosa include the participation of a phosphoenolpyruvate:fructose 1-phosphotransferase system (F-PTS). Fractionation of crude extracts of fructose-grown cells revealed that both membrane-associated and soluble components were essential fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.2.534-541.1982

    authors: Durham DR,Phibbs PV Jr

    更新日期:1982-02-01 00:00:00

  • The poc locus is required for 1,2-propanediol-dependent transcription of the cobalamin biosynthetic (cob) and propanediol utilization (pdu) genes of Salmonella typhimurium.

    abstract::In this communication we present evidence that indicates that 1,2-propanediol (1,2-PDL) is a positive effector of the transcription of the cobalamin (vitamin B12) biosynthetic (cob) operon and of the 1,2-PDL utilization (pdu) genes. The stimulatory effects of 1,2-PDL were demonstrated using Mu d-lac transcriptional fu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.7.2267-2272.1992

    authors: Rondon MR,Escalante-Semerena JC

    更新日期:1992-04-01 00:00:00

  • Size comparisons among integral membrane transport protein homologues in bacteria, Archaea, and Eucarya.

    abstract::Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.3.1012-1021.2001

    authors: Chung YJ,Krueger C,Metzgar D,Saier MH Jr

    更新日期:2001-02-01 00:00:00

  • Transcriptional regulation of the ilv-leu operon of Bacillus subtilis.

    abstract::We used primer extension and mutational analysis to identify a promoter upstream of ilvB, the first gene in the ilv-leu operon of Bacillus subtilis. Between the promoter and ilvB, there is a 482-bp leader region which contains a sequence that resembles a factor-independent transcription terminator. In in vitro transcr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.10.3212-3219.1992

    authors: Grandoni JA,Zahler SA,Calvo JM

    更新日期:1992-05-01 00:00:00

  • NOD2/c-Jun NH2-Terminal Kinase Triggers Mycoplasma ovipneumoniae-Induced Macrophage Autophagy.

    abstract::Mycoplasma ovipneumoniae belongs to Mycoplasma, a genus containing the smallest self-replicating microorganisms, and causes infectious pleuropneumonia in goats and sheep. Nucleotide-binding oligomerization domain-containing protein (NOD2), an intracellular pattern recognition receptor, interacts with muramyl dipeptide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00689-19

    authors: Luo H,Wu X,Xu Z,Hao X,Wang Y,Li M

    更新日期:2020-09-23 00:00:00

  • Highly repetitive DNA sequences in cyanobacterial genomes.

    abstract::We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2755-2761.1990

    authors: Mazel D,Houmard J,Castets AM,Tandeau de Marsac N

    更新日期:1990-05-01 00:00:00

  • Macromolecular composition of spores from the filamentous cyanobacterium A nabaena cylindrica.

    abstract::Spores were isolated from the filamentous cyanobacterium Anabaena cylindrica, and their deoxyribonucleic acid, ribonucleic acid, and protein compositions were determined. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.2.1154-1155.1977

    authors: Simon RD

    更新日期:1977-02-01 00:00:00

  • Cyclic diguanylic acid and cellulose synthesis in Agrobacterium tumefaciens.

    abstract::The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.12.6649-6655.1989

    authors: Amikam D,Benziman M

    更新日期:1989-12-01 00:00:00

  • Formation of protoporphyrin from coproporphyrinogen in extracts of various bacteria.

    abstract::The enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of Pseudomonas fluorescens grown aerobically, of P. denitrificans grown anaerobically under denitrifying conditions, and of Escherichia coli grown both aerobically and anaerobically. Protop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.398-403.1970

    authors: Jacobs NJ,Jacobs JM,Brent P

    更新日期:1970-05-01 00:00:00

  • Events following prophage Mu induction.

    abstract::Escherichia coli strains lysogenic for a thermoinducible Mu prophage (Mu cts62) undergo rapid lysis about 50 min after heat induction. Induction of Mu cts62 apparently causes damage to the host sequences in which Mu is inserted. The normal expression of A, BU, and X genes of Mu is needed for this specific deleterious ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.2.437-442.1975

    authors: Razzaki T,Bukhari AI

    更新日期:1975-05-01 00:00:00

  • Induction of mutants of Staphylococcus aureus 100 with increased ability to produce enterotoxin A.

    abstract::As a result of serial exposures to a mutagenic agent, N-methyl-N'-nitro-N-nitrosoguanidine, the yield of enterotoxin A produced by the last mutant in the series was increased nearly 20-fold over the amount produced by the parent Staphylococcus aureus 100. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.1.289-291.1971

    authors: Friedman ME,Howard MB

    更新日期:1971-04-01 00:00:00

  • Rotation and switching of the flagellar motor assembly in Halobacterium halobium.

    abstract::Halobacterium halobium swims with a polarly inserted motor-driven flagellar bundle. The swimming direction of the cell can be reserved by switching the rotational sense of the bundle. The switch is under the control of photoreceptor and chemoreceptor proteins that act through a branched signal chain. The swimming beha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.6.1971-1977.1991

    authors: Marwan W,Alam M,Oesterhelt D

    更新日期:1991-03-01 00:00:00

  • Production of large amounts of acetate during germination of Bacillus megaterium spores in the absence of exogenous carbon sources.

    abstract::When Bacillus megaterium spores germinate in the absence of an exogenous carbon source, the first minutes of germination are accompanied by production of large amounts (approximately 70 nmol/mg of dry spores) of acetate and much smaller amounts of pyruvate and lactate. The majority of these compounds are excreted into...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.744-746.1977

    authors: Setlow B,Shay LK,Vary JC,Setlow P

    更新日期:1977-11-01 00:00:00

  • Competitive inhibition of transformation in group H Streptococcus strain Challis by heterologous deoxyribonucleic acid.

    abstract::Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1621-1623.1975

    authors: Ceglowski P,Fuchs PG,Soltyk A

    更新日期:1975-12-01 00:00:00

  • Identification of phthiodiolone ketoreductase, an enzyme required for production of mycobacterial diacyl phthiocerol virulence factors.

    abstract::Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.14.4760-4766.2005

    authors: Onwueme KC,Vos CJ,Zurita J,Soll CE,Quadri LE

    更新日期:2005-07-01 00:00:00