Transcriptional regulation of the ilv-leu operon of Bacillus subtilis.

Abstract:

:We used primer extension and mutational analysis to identify a promoter upstream of ilvB, the first gene in the ilv-leu operon of Bacillus subtilis. Between the promoter and ilvB, there is a 482-bp leader region which contains a sequence that resembles a factor-independent transcription terminator. In in vitro transcription experiments, 90% of transcripts initiated at the ilvB promoter ended at a site near this terminator. Primer extension analysis of RNA synthesized in vivo showed that the steady-state level of mRNA upstream of the terminator was twofold higher from cells limited for leucine than it was from cells grown with excess leucine. mRNA downstream of the terminator was 14-fold higher in cells limited for leucine than in cells grown with excess leucine. Measurement of mRNA degradation rates showed that the half-life of ilv-leu mRNA was the same when the cells were grown with or without leucine. These data demonstrate that the ilv-leu operon is regulated by transcription attenuation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Grandoni JA,Zahler SA,Calvo JM

doi

10.1128/jb.174.10.3212-3219.1992

keywords:

subject

Has Abstract

pub_date

1992-05-01 00:00:00

pages

3212-9

issue

10

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

杂志文章
  • Serological comparison of ten glycolytic Mycoplasma species.

    abstract::Seventeen strains of mycoplasmata representing 11 named species were compared serologically by three parameters: growth inhibition on agar, double immunodiffusion, and complement fixation. In growth-inhibition studies, a strain labeled Mycoplasma histotropicum was found related to and perhaps best classified as M. pul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.1044-1055.1969

    authors: Kenny GE

    更新日期:1969-06-01 00:00:00

  • The C terminus of sigma(32) is not essential for degradation by FtsH.

    abstract::A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.20.5911-5917.2001

    authors: Tomoyasu T,Arsène F,Ogura T,Bukau B

    更新日期:2001-10-01 00:00:00

  • Cloning and characterization of a glutamine transport operon of Bacillus stearothermophilus NUB36: effect of temperature on regulation of transcription.

    abstract::We cloned and sequenced a fragment of the Bacillus stearothermophilus NUB36 chromosome that contains two open reading frames (ORFs) whose products were detected only in cells of cultures grown in complex medium at high temperature. The nucleotide sequence of the two ORFs exhibited significant identity to the sequence ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.15.4877-4888.1991

    authors: Wu L,Welker NE

    更新日期:1991-08-01 00:00:00

  • Cytochemical evidence of extramembranous carbohydrates on Ureaplasma urealyticum (T-strain Mycoplasma).

    abstract::Cells of Ureaplasma urealyticum that were prepared by a ruthenium red technique demonstrated an extramembranous layer of polyanions of about 15 to 30 nm in width. Application of the concanavalin A-iron dextran strain indicated that the outer surface of this layer contained glucosyl-like residues. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.2.658-660.1976

    authors: Robertson J,Smook E

    更新日期:1976-11-01 00:00:00

  • Isolation and characterization of the Escherichia coli htrD gene, whose product is required for growth at high temperatures.

    abstract::Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chrom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.4.1240-1247.1992

    authors: Delaney JM,Ang D,Georgopoulos C

    更新日期:1992-02-01 00:00:00

  • Extrachromosomal deoxyribonucleic acid in different enterobacteria.

    abstract::Eighty-seven different enterobacteria and pseudomonas strains were examined for the presence of extrachromosomal deoxyribonucleic acid (DNA). Thirty-four strains contained closed circular DNA by the ethidium bromide CsCl density technique. Extrachromosomal DNA was most frequent in Escherichia and Klebsiella strains. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.1.367-377.1973

    authors: Christiansen C,Christiansen G,Bak AL,Stenderup A

    更新日期:1973-04-01 00:00:00

  • Swarming: flexible roaming plans.

    abstract::Movement over an agar surface via swarming motility is subject to formidable challenges not encountered during swimming. Bacteria display a great deal of flexibility in coping with these challenges, which include attracting water to the surface, overcoming frictional forces, and reducing surface tension. Bacteria that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.02063-12

    authors: Partridge JD,Harshey RM

    更新日期:2013-03-01 00:00:00

  • Cloning of the gene for myxobacterial hemagglutinin and isolation and analysis of structural gene mutations.

    abstract::Myxobacterial hemagglutinin (MBHA) is a major developmentally induced protein that accumulates during the period of cellular aggregation in the bacterium Myxococcus xanthus. It has been shown that this lectin is targeted to the cell surface and periplasmic space of developmental cells, suggesting that it may play a ro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.8.3801-3808.1987

    authors: Romeo JM,Zusman DR

    更新日期:1987-08-01 00:00:00

  • Neutralization of endotoxin toxicity in chick embryos by antibiotics.

    abstract::Rifkind, David (University of Colorado Medical Center, Denver), and John D. Palmer. Neutralization of endotoxin toxicity in chick embryos by antibiotics. J. Bacteriol. 92:815-819. 1966.-Three cationic cyclic polypeptide antibiotics, polymyxin B sulfate colistin sulfate, and tyrocidine hydrochloride, were shown to neut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.4.815-819.1966

    authors: Rifkind D,Palmer JD

    更新日期:1966-10-01 00:00:00

  • Thermoregulation of virB transcription in Shigella flexneri by sensing of changes in local DNA superhelicity.

    abstract::Transcription of the virB gene, a transcriptional regulator of invasion genes on the large plasmid of Shigella flexneri, is strictly regulated by growth temperature; when bacteria are grown at 37 degrees C, virB transcription is highly activated, while at 30 degrees C the level of virB transcription decreases to less ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.4.1094-1097.1995

    authors: Tobe T,Yoshikawa M,Sasakawa C

    更新日期:1995-02-01 00:00:00

  • Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus.

    abstract::We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the regulatory region of the operon and the gene encoding the specific regulator of D-serine tolerance (dsdC) had been misplaced ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6456-6461.1995

    authors: Nørregaard-Madsen M,McFall E,Valentin-Hansen P

    更新日期:1995-11-01 00:00:00

  • Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170.

    abstract::The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.6.1776-1782.2003

    authors: Uchiyama T,Kaneko R,Yamaguchi J,Inoue A,Yanagida T,Nikaidou N,Regue M,Watanabe T

    更新日期:2003-03-01 00:00:00

  • Role of the CheW protein in bacterial chemotaxis: overexpression is equivalent to absence.

    abstract::The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.11.6271-6278.1989

    authors: Sanders DA,Mendez B,Koshland DE Jr

    更新日期:1989-11-01 00:00:00

  • The essential β-barrel assembly machinery complex components BamD and BamA are required for autotransporter biogenesis.

    abstract::Autotransporter biogenesis is dependent upon BamA, a central component of the β-barrel assembly machinery (BAM) complex. In this report, we detail the role of the other BAM components (BamB-E). We identify the importance of BamD in autotransporter biogenesis and show that BamB, BamC, and BamE are not required. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00192-11

    authors: Rossiter AE,Leyton DL,Tveen-Jensen K,Browning DF,Sevastsyanovich Y,Knowles TJ,Nichols KB,Cunningham AF,Overduin M,Schembri MA,Henderson IR

    更新日期:2011-08-01 00:00:00

  • Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene.

    abstract::The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.5789-5794.1990

    authors: David S,van der Rest ME,Driessen AJ,Simons G,de Vos WM

    更新日期:1990-10-01 00:00:00

  • Effect of Lipidation on the Localization and Activity of a Lysozyme Inhibitor in Neisseria gonorrhoeae.

    abstract::The Gram-negative pathogen Neisseria gonorrhoeae (gonococcus [Gc]) colonizes lysozyme-rich mucosal surfaces. Lysozyme hydrolyzes peptidoglycan, leading to bacterial lysis. Gc expresses two proteins, SliC and NgACP, that bind and inhibit the enzymatic activity of lysozyme. SliC is a surface-exposed lipoprotein, while N...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00633-19

    authors: Ragland SA,Gray MC,Melson EM,Kendall MM,Criss AK

    更新日期:2020-03-26 00:00:00

  • Environmental role of pathogenic traits in Vibrio cholerae.

    abstract::Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00795-17

    authors: Sakib SN,Reddi G,Almagro-Moreno S

    更新日期:2018-03-26 00:00:00

  • A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport.

    abstract::The Yersinia adhesin A (YadA) is a trimeric autotransporter adhesin of enteric yersiniae. It consists of three major domains: a head mediating adherence to host cells, a stalk involved in serum resistance, and an anchor that forms a membrane pore and is responsible for the autotransport function. The anchor contains a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00985-07

    authors: Grosskinsky U,Schütz M,Fritz M,Schmid Y,Lamparter MC,Szczesny P,Lupas AN,Autenrieth IB,Linke D

    更新日期:2007-12-01 00:00:00

  • CATALASE ACTIVITY IN ANAPLASMA MARGINALE.

    abstract::Wallace, W. R. (Louisiana State University, Baton Rouge), and G. T. Dimopoullos. Catalase activity in Anaplasma marginale. J. Bacteriol. 91:309-311. 1965.-Extracts of erythrocytes infected with Anaplasma marginale were found to contain more catalase activity than normal erythrocytic preparations. The increase in catal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.309-311.1965

    authors: WALLACE WR,DIMOPOULLOS GT

    更新日期:1965-08-01 00:00:00

  • Effect of growth medium on the relative polypeptide composition of cellular outer membrane and released outer membrane material in Escherichia coli.

    abstract::When ratios of the major polypeptides of the outer membrane isolated from cells of Escherichia coli B grown in minimal medium containing either a single amino acid or several amino acids were compared, no difference was observed. However, the ratio of these polypeptides in outer membrane material released into the med...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.2.1031-1034.1979

    authors: Loeb MR,Kilner J

    更新日期:1979-02-01 00:00:00

  • Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system.

    abstract::Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.1.197-203.1966

    authors: Day LE

    更新日期:1966-07-01 00:00:00

  • Superdormant spores of Bacillus species have elevated wet-heat resistance and temperature requirements for heat activation.

    abstract::Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00736-09

    authors: Ghosh S,Zhang P,Li YQ,Setlow P

    更新日期:2009-09-01 00:00:00

  • tRNA Modification Profiles and Codon-Decoding Strategies in Methanocaldococcus jannaschii.

    abstract::tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00690-18

    authors: Yu N,Jora M,Solivio B,Thakur P,Acevedo-Rocha CG,Randau L,de Crécy-Lagard V,Addepalli B,Limbach PA

    更新日期:2019-04-09 00:00:00

  • The virulence regulator Sae of Staphylococcus aureus: promoter activities and response to phagocytosis-related signals.

    abstract::The two-component system SaeRS of Staphylococcus aureus is closely involved in the regulation of major virulence factors. However, little is known about the signals leading to saeRS activation. A total of four overlapping transcripts (T1 to T4) from three different transcription starting points are expressed in the sa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01927-07

    authors: Geiger T,Goerke C,Mainiero M,Kraus D,Wolz C

    更新日期:2008-05-01 00:00:00

  • Identification of the lipopolysaccharide core region as the receptor site for a cytotoxin-converting phage, phi CTX, of Pseudomonas aeruginosa.

    abstract::A temperate phage, phi CTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of phi CTX-resistant mutants derived from phi CTX-sensitive strains. phi CTX infectivity was neutralized by LPS preparations derived from sensitive strains but ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.17.5262-5269.1994

    authors: Yokota S,Hayashi T,Matsumoto H

    更新日期:1994-09-01 00:00:00

  • Genetic, molecular, and functional analysis of Streptococcus faecalis R plasmid pJH1.

    abstract::Streptococcus faecalis JH1 contains two conjugative plasmids, pJH1, an R plasmid that codes for resistance to kanamycin, streptomycin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Strain JH1 was used as an antibiotic resistance donor in conjugation experiments with two plasmid-free S. fae...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1094-1104.1983

    authors: Banai M,LeBlanc DJ

    更新日期:1983-09-01 00:00:00

  • Genome sequence of Klebsiella pneumoniae LZ, a potential platform strain for 1,3-propanediol production.

    abstract::Klebsiella pneumoniae LZ is a bacterium isolated from soil which can produce 1,3-propanediol from glycerol. Here we present a 5,431,750-bp assembly of its genome sequence. We annotated 9 coding sequences (CDSs) responsible for glycerol fermentation to 1,3-propanediol, 19 CDSs encoding glycerol utilization, and 134 CDS...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00864-12

    authors: Tao F,Tai C,Liu Z,Wang A,Wang Y,Li L,Gao C,Ma C,Xu P

    更新日期:2012-08-01 00:00:00

  • Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli.

    abstract::DNA sequence analysis of the modABCD operon of Escherichia coli revealed the presence of four open reading frames. The first gene, modA, codes for a 257-amino-acid periplasmic binding protein enunciated by the presence of a signal peptide-like sequence. The second gene (modB) encodes a 229-amino-acid protein with a po...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.17.4851-4856.1995

    authors: Maupin-Furlow JA,Rosentel JK,Lee JH,Deppenmeier U,Gunsalus RP,Shanmugam KT

    更新日期:1995-09-01 00:00:00

  • An antimicrobial peptide is produced by extracellular processing of a protein from Propionibacterium jensenii.

    abstract::A protease-activated antimicrobial peptide (PAMP) and its inactive precursor were purified from the culture supernatant of Propionibacterium jensenii LMG 3032 and characterized at the molecular level. PAMP is a 64-amino-acid cationic peptide of 6,383 Da with physicochemical features similar to those of bacteriocins fr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.13.3649-3656.2002

    authors: Faye T,Brede DA,Langsrud T,Nes IF,Holo H

    更新日期:2002-07-01 00:00:00

  • Outer membrane proteins and cell surface structure of Selenomonas ruminantium.

    abstract::The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first, isoelectric focusing; second, sodium dodecyl sulfate) polyacrylamide slab gel electrophoresis. The outer membrane from both glucos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.899-907.1980

    authors: Kamio Y,Takahashi H

    更新日期:1980-02-01 00:00:00