Thermoregulation of virB transcription in Shigella flexneri by sensing of changes in local DNA superhelicity.

abstract：:The entry of oligogalacturonates into Dickeya dadantii occurs through the specific channel KdgM. The genome of the bacterium encodes a second member of this family of outer membrane proteins, KdgN. We showed that this protein is also involved in the uptake of oligogalacturonates. When KdgN was reconstituted in proteol...

journal_title：Journal of bacteriology

authors： Condemine G,Ghazi A

更新日期：2007-08-01 00:00:00

abstract：:The DnaA protein concentration in Escherichia coli was increased above the wild-type level by inducing a lacP-controlled dnaA gene located on a plasmid. In these cells with different DnaA protein levels, we measured several parameters: dnaA gene expression; cell size, amount of DNA per cell, and number of origins per ...

journal_title：Journal of bacteriology

authors： Atlung T,Hansen FG

更新日期：1993-10-01 00:00:00

abstract：:Streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. This study was initiated by the finding that IgM degradation products are released after opsonization of S. suis. The objective of this work was to identify the bacterial factor re...

journal_title：Journal of bacteriology

authors： Seele J,Singpiel A,Spoerry C,von Pawel-Rammingen U,Valentin-Weigand P,Baums CG

更新日期：2013-03-01 00:00:00

abstract：:In Bacillus subtilis, carbon catabolite repression (CCR) of many genes is mediated at cis-acting carbon repression elements (cre) by the catabolite repressor protein CcpA. Mutations in transcription-repair coupling factor (mfd) partially relieve CCR at cre sites located downstream of transcriptional start sites by abo...

journal_title：Journal of bacteriology

authors： Zalieckas JM,Wray LV Jr,Fisher SH

更新日期：1998-12-01 00:00:00

abstract：:Sensory adaptation by the chemotaxis system of Escherichia coli requires adjustments of the extent of methyl esterification of the chemotaxis receptor proteins. One mechanism utilized by E. coli to make such adjustments is to control the activity of CheB, the enzyme responsible for removing receptor methyl ester group...

journal_title：Journal of bacteriology

authors： Stewart RC,Roth AF,Dahlquist FW

更新日期：1990-06-01 00:00:00

abstract：:A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. ...

journal_title：Journal of bacteriology

authors： Eddy CK,Smith OH,Noel KD

更新日期：1988-07-01 00:00:00

abstract：:Low-copy-number plasmids, such as P1 prophage and the fertility factor F, require a plasmid-encoded replication protein and several host products for replication. Stable maintenance also depends on active partitioning of plasmids into daughter cells. Mini-P1 par+ and par plasmids were found to be destabilized by mutat...

journal_title：Journal of bacteriology

authors： Tilly K,Yarmolinsky M

更新日期：1989-11-01 00:00:00

abstract：:Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...

journal_title：Journal of bacteriology

authors： ISHIBASHI M,SUGINO Y,HIROTA Y

更新日期：1964-03-01 00:00:00

abstract：:Previous studies have shown that narL+ is required for nitrate induction of nitrate reductase synthesis and for nitrate inhibition of fumarate reductase synthesis in Escherichia coli. We cloned narL on a 5.1-kilobase HindIII fragment. Our clone also contained a previously unidentified gene, which we propose to designa...

journal_title：Journal of bacteriology

authors： Stewart V,Parales J Jr

更新日期：1988-04-01 00:00:00

abstract：:The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...

journal_title：Journal of bacteriology

authors： Beck C,von Meyenburg HK

更新日期：1968-08-01 00:00:00

abstract：:The Escherichia coli LytB protein regulates the activity of guanosine 3',5'-bispyrophosphate synthetase I (RelA). A Southern blot analysis of chromosomal DNA with the E. coli lytB gene as a probe revealed the presence of lytB homologs in all of the gram-negative bacterial species examined but not in gram-positive spec...

journal_title：Journal of bacteriology

authors： Potter S,Yang X,Boulanger MJ,Ishiguro EE

更新日期：1998-04-01 00:00:00

abstract：:A citrate lyase (EC 4.1.3.6) was purified 25-fold from Leuconostoc mesenteroides and was shown to contain three subunits. The first 42 amino acids of the beta subunit were identified, as well as an internal peptide sequence spanning some 20 amino acids into the alpha subunit. Using degenerated primers from these seque...

journal_title：Journal of bacteriology

authors： Bekal S,Van Beeumen J,Samyn B,Garmyn D,Henini S,Diviès C,Prévost H

更新日期：1998-02-01 00:00:00

abstract：:Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions...

journal_title：Journal of bacteriology

authors： Kosugi A,Murashima K,Doi RH

更新日期：2001-12-01 00:00:00

abstract：:The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...

journal_title：Journal of bacteriology

authors： Wu CY,Blaszczak LC,Smith MC,Skatrud PL

更新日期：1994-03-01 00:00:00

abstract：:Most strains of Pseudomonas aeruginosa can express two chemically and immunologically distinct types of lipopolysaccharide (LPS), an antigenically conserved form called A band and the serotype-specific form called B band. To study the molecular controls regulating expression of the A-band LPS antigen, we have cloned t...

journal_title：Journal of bacteriology

authors： Lightfoot J,Lam JS

更新日期：1991-09-01 00:00:00

abstract：:We purified membrane-bound cytochrome c-550 [cytochrome c-550(m)] to an electrophoretically homogeneous state from Nitrobacter winogradskyi. The cytochrome showed peaks at 409 and 525 nm in the oxidized form and peaks at 416, 521, and 550 nm in the reduced form. The molecular weight of the cytochrome was estimated to ...

journal_title：Journal of bacteriology

authors： Nomoto T,Fukumori Y,Yamanaka T

更新日期：1993-07-01 00:00:00

abstract：UNLABELLED:It is well established that the RpoN-RpoS sigma factor (σ(54)-σ(S)) cascade plays an essential role in differential gene expression during the enzootic cycle of Borrelia burgdorferi, the causative agent of Lyme disease. The RpoN-RpoS pathway is activated by the response regulator/σ(54)-dependent activator (a...

journal_title：Journal of bacteriology

authors： Yin Y,Yang Y,Xiang X,Wang Q,Yang ZN,Blevins J,Lou Y,Yang XF

更新日期：2016-04-28 00:00:00

abstract：:A genetic approach was undertaken to identify normal bacterial genes whose products function to limit the effective concentration of antibiotics. In this approach, a multicopy plasmid library containing cloned Escherichia coli chromosomal sequences was screened for transformants that showed increased resistance to a n...

journal_title：Journal of bacteriology

authors： Gambino L,Gracheck SJ,Miller PF

更新日期：1993-05-01 00:00:00

abstract：:Strains monosomic for chromosome I of Saccharomyces cerevisiae contain 25 to 35% fewer rRNA genes than do normal diploid strains. When these strains are repeatedly subcultured, colonies are isolated that have magnified their number of rRNA genes to the diploid amount while remaining monosomic for chromosome I. We have...

journal_title：Journal of bacteriology

authors： Kaback DB,Halvorson HO

更新日期：1978-04-01 00:00:00

abstract：:Lipopolysaccharide (LPS) fractions were isolated from three species of blue-green algae of the genus Phormidium, namely, P. africanum, P. laminosum, and P. uncinatum, by using a phenol-water procedure followed by exhaustive extraction with ammonium oxalate. The materials obtained were shown to be closely related bioch...

journal_title：Journal of bacteriology

authors： Mikheyskaya LV,Ovodova RG,Ovodov YS

更新日期：1977-04-01 00:00:00

abstract：:The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of significant homology that lie wit...

journal_title：Journal of bacteriology

authors： Jackson MP,Deresiewicz RL,Calderwood SB

更新日期：1990-06-01 00:00:00

abstract：:The thermosensitive growth phenotype exerted by runaway-mutant plasmids was suppressed by sublethal doses of the DNA gyrase inhibitors novobiocin or nalidixic acid, although the latter drug was less efficient. A novobiocin-resistant gyrB mutant Escherichia coli strain prevented expression of the runaway phenotype at 3...

journal_title：Journal of bacteriology

authors： Uhlin BE,Nordström K

更新日期：1985-05-01 00:00:00

abstract：:The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...

journal_title：Journal of bacteriology

authors： Belitsky BR,Sonenshein AL

更新日期：1998-12-01 00:00:00

abstract：:The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...

journal_title：Journal of bacteriology

authors： Rodriguez RL,Davern CI

更新日期：1976-01-01 00:00:00

abstract：:Tetracycline accumulation by the phototrophic bacterium Rhodopseudomonas sphaeroides has been studied, using the fluorescence properties of the antibiotic and measuring uptake of [7- 3H]tetracycline. Accumulation was carrier mediated, with a Km of approximately 300 micronM. Efflux also appeared to be carried mediated,...

journal_title：Journal of bacteriology

authors： Weckesser J,Magnuson JA

更新日期：1979-06-01 00:00:00

abstract：:The metabolism of mRNA from the lactose (lac) operon of Escherichia coli has been studied in ribonuclease (RNase) III-deficient strains (rnc-105). The induction lag for beta-galactosidase from the first gene was twice as long, and enzyme synthesis was reduced 10-fold in one such mutant compared with its isogenic rnc+ ...

journal_title：Journal of bacteriology

authors： Talkad V,Achord D,Kennell D

更新日期：1978-08-01 00:00:00

abstract：:A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12. ...

journal_title：Journal of bacteriology

authors： Dickinson ES,Sundaram TK

更新日期：1970-03-01 00:00:00

abstract：:Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...

journal_title：Journal of bacteriology

authors： Price LB,Shand RF

更新日期：2000-09-01 00:00:00

abstract：:The gene encoding OprF, a major outer membrane protein in Pseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigm...

journal_title：Journal of bacteriology

authors： Brinkman FS,Schoofs G,Hancock RE,De Mot R

更新日期：1999-08-01 00:00:00

abstract：:There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...

journal_title：Journal of bacteriology

authors： Gibson JL,Tabita FR

更新日期：1985-12-01 00:00:00