Isolation and characterization of lipopolysaccharides from cell walls of blue-green algae of the genus Phormidium.

Abstract:

:Lipopolysaccharide (LPS) fractions were isolated from three species of blue-green algae of the genus Phormidium, namely, P. africanum, P. laminosum, and P. uncinatum, by using a phenol-water procedure followed by exhaustive extraction with ammonium oxalate. The materials obtained were shown to be closely related biochemically. Nearly 60% of the LPS consisted of the polysaccharides galactose, glucose, mannose, xylose, arabinose, and rhamnose and an unidentified, fast-moving sugar residue. In addition, glucosamine, galactosamine, and 2-keto-3-deoxyoctonate were detected. Oleic, stearic, and palmitic acids were found in the hydrolysate of the lipid component, which averaged 1.5% of the LPS. Concomitantly, the protein component (7 to 20%) was shown to contain the following amino acids: aspartic acid, threonine, serine glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, and arginine. Whole cells, as well as the LPS, of Phormidium possessed antigenic properties.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Mikheyskaya LV,Ovodova RG,Ovodov YS

doi

10.1128/JB.130.1.1-3.1977

subject

Has Abstract

pub_date

1977-04-01 00:00:00

pages

1-3

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

130

pub_type

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