Generic identification of L-forms by polyacrylamide gel electrophoretic comparison of extracts from parent strains and their derived L-forms.

Abstract:

:A polyacrylamide gel electrophoretic method was used for identification of L-forms in the genera Streptococcus, Staphylococcus, and Proteus, by comparing phenol-acetic acid-water extracts of homologous parent L-form pairs to one another and to other pairs. The method requires only milligram quantities of material for analysis. The standard patterns for the strains used in this study are shown as pictures of the gels and as densitometric tracings of appropriate gels. They show that, despite occasional minor differences in some organisms, the gel electrophoretic patterns of homologous L-forms and bacterial pairs are sufficiently similar-as well as sufficiently dissimilar from patterns of other genera-to permit generic identification of an unknown L-form by reference to patterns derived from possible parental bacteria.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

King JR,Theodore TS,Cole RM

doi

10.1128/JB.100.1.71-77.1969

subject

Has Abstract

pub_date

1969-10-01 00:00:00

pages

71-7

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

100

pub_type

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