Abstract:
:A polyacrylamide gel electrophoretic method was used for identification of L-forms in the genera Streptococcus, Staphylococcus, and Proteus, by comparing phenol-acetic acid-water extracts of homologous parent L-form pairs to one another and to other pairs. The method requires only milligram quantities of material for analysis. The standard patterns for the strains used in this study are shown as pictures of the gels and as densitometric tracings of appropriate gels. They show that, despite occasional minor differences in some organisms, the gel electrophoretic patterns of homologous L-forms and bacterial pairs are sufficiently similar-as well as sufficiently dissimilar from patterns of other genera-to permit generic identification of an unknown L-form by reference to patterns derived from possible parental bacteria.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
King JR,Theodore TS,Cole RMdoi
10.1128/JB.100.1.71-77.1969subject
Has Abstractpub_date
1969-10-01 00:00:00pages
71-7issue
1eissn
0021-9193issn
1098-5530journal_volume
100pub_type
杂志文章abstract::Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5027-5033.1988
更新日期:1988-11-01 00:00:00
abstract::In Listeria monocytogenes, the full details of how stress signals are integrated into the σB regulatory pathway are not yet available. To help shed light on this question, we investigated a collection of transposon mutants that were predicted to have compromised activity of the alternative sigma factor B (σB). These m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00751-19
更新日期:2020-04-09 00:00:00
abstract::Transcriptional regulation of the Mg(2+)-citrate transporter, CitM, the main citrate uptake system of Bacillus subtilis, was studied during growth in rich medium. Citrate in the growth medium was required for induction under all growth conditions. In Luria-Bertani medium containing citrate, citM expression was complet...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.3.854-859.2003
更新日期:2003-02-01 00:00:00
abstract::The bacteriocin produced by a strain of Staphylococcus aureus has been isolated and designated staphylococcin (414), and a study was made of its chemical, physical, and biological properties. The staphylococcin is released in appreciable quantities after breakage of the cells and can be purified through differential c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.117-125.1970
更新日期:1970-10-01 00:00:00
abstract::In Rhodobacter sphaeroides, MreB, MreC, MreD, PBP2, and RodA are encoded at the same locus. The localizations of PBP2, MreB, and MreC, which have all been implicated in the synthesis of the peptidoglycan layer, were investigated under different growth conditions to gain insight into the relationships between these pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.5.1691-1700.2006
更新日期:2006-03-01 00:00:00
abstract::The human pathogen Vibrio cholerae carries a chromosomal superintegron (SI). The SI contains an array of hundreds of gene cassettes organized in tandem which are stable under conditions when no particular stress is applied to bacteria (such as during laboratory growth). Rearrangements of these cassettes are catalyzed ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05982-11
更新日期:2012-04-01 00:00:00
abstract::Commonly used procedures effected the extraction of only 10% of the lipoteichoic acid of stationary-phase cells of Staphylococcus aureus and Streptococcus faecium, unless the cells were first disrupted. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.399-402.1982
更新日期:1982-01-01 00:00:00
abstract::The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.20.5625-5632.2002
更新日期:2002-10-01 00:00:00
abstract::Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00075-12
更新日期:2012-04-01 00:00:00
abstract::The general aromatic amino acid permease, AroP, of Escherichia coli is responsible for the active transport of phenylalanine, tyrosine, and tryptophan. A proposed topological model for the AroP permease, consisting of 12 hydrophobic transmembrane spans connected by hydrophilic loops, is very similar to that of the clo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.10.3317-3323.1997
更新日期:1997-05-01 00:00:00
abstract::The first common enzyme of isoleucine and valine biosynthesis, acetolactate synthase (ALS), is specifically inhibited by the herbicide sulfometuron methyl (SM). To further understand the physiological consequences of flux alterations at this point in metabolism, Escherichia coli genes whose expression was induced by p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.785-792.1998
更新日期:1998-02-01 00:00:00
abstract::A temperate phage, phi CTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of phi CTX-resistant mutants derived from phi CTX-sensitive strains. phi CTX infectivity was neutralized by LPS preparations derived from sensitive strains but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.17.5262-5269.1994
更新日期:1994-09-01 00:00:00
abstract::The phoP-phoQ operon of Salmonella typhimurium is a member of the family of two-component regulatory systems and controls expression of the phoN gene that codes for nonspecific acid phosphatase and the genes involved in the pathogenicity of the bacterium. The phoP-phoQ operon of Escherichia coli was cloned on a plasmi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.492-498.1992
更新日期:1992-01-01 00:00:00
abstract::The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.426-433.1982
更新日期:1982-02-01 00:00:00
abstract::Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.404-410.1970
更新日期:1970-05-01 00:00:00
abstract::The Escherichia coli K42 capsular polysaccharide consists of leads to 3)-alpha-D-Galp-(1 leads to 3)-alpha-D-GalUAp-(1 leads to 3)-alpha-L-Fucp-(1 leads to repeating units. The E. coli K42 and Klebsiella K63 antigens are serologically identical. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.390-391.1978
更新日期:1978-01-01 00:00:00
abstract::Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.4.1145-1150.1964
更新日期:1964-10-01 00:00:00
abstract::The fungal pathogen Cryptococcus neoformans is killed by the bacterium Staphylococcus aureus, and the killing is inhibited by soluble capsular polysaccharides. To investigate the mechanism of killing, cells in coculture were examined by scanning and transmission electron microscopy. S. aureus attached to the capsule o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00412-07
更新日期:2007-07-01 00:00:00
abstract::Deletions of the Escherichia coli K-12 chromosome between trpR and thr render the bacterium sensitive to the dye toluidine blue (Dye-), and if male (Hfr or F'), the strain is sterile (Fex-), failing to donate F' or chromosomal markers and resistant to male-specific phages as a consequence of its inability to elaborate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1309-1314.1983
更新日期:1983-06-01 00:00:00
abstract::Microbial communities span many orders of magnitude, ranging in scale from hundreds of cells on a single particle of soil to billions of cells within the lumen of the gastrointestinal tract. Bacterial cells in all habitats are members of densely populated local environments that facilitate competition between neighbor...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00275-16
更新日期:2016-07-28 00:00:00
abstract::Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1628-1633.1990
更新日期:1990-03-01 00:00:00
abstract::The kinetics of respiration-dependent proton efflux and membrane energization have been studied in intact cells of logarithmic-phase Escherichia coli. Parallel measurements of the rate and extent of proton efflux into the external medium (half-time, about 10 s; ratio of H(+) to O, about 0.5) and the oxidation of E. co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.176-184.1979
更新日期:1979-04-01 00:00:00
abstract::The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6207-6214.1992
更新日期:1992-10-01 00:00:00
abstract::Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.577-581.1963
更新日期:1963-09-01 00:00:00
abstract::Picolinic acid (PA), a typical C-2-carboxylated pyridine derivative, is a metabolite of l-tryptophan and many other aromatic compounds in mammalian and microbial cells. Microorganisms can degrade and utilize PA for growth. However, the precise mechanism of PA metabolism remains unknown. Alcaligenes faecalis strain JQ1...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00665-18
更新日期:2019-03-13 00:00:00
abstract::Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.2.329-334.1970
更新日期:1970-08-01 00:00:00
abstract::Salmonella enterica can utilize fructose-asparagine (F-Asn) as a source of carbon and nitrogen. This capability has been attributed to five genes in the fra locus. Previously, we determined that mutations in fraB (deglycase), fraD (kinase), or fraA (transporter) eliminated the ability of Salmonella to grow on F-Asn, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00330-17
更新日期:2017-10-17 00:00:00
abstract::We reported previously that a Salmonella enterica serovar Enteritidis dam mutant expressing a truncated Dam protein does not agglutinate in the presence of specific antibodies against O9 polysaccharide. Here we investigate the participation of Dam in lipopolysaccharide (LPS) synthesis in Salmonella. The LPS O-antigen ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00839-09
更新日期:2009-11-01 00:00:00
abstract::The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.330-333.1984
更新日期:1984-01-01 00:00:00
abstract::Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.23.7346-7355.1999
更新日期:1999-12-01 00:00:00