Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism.

Abstract:

:Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO) as the probe, identified at least four plasmids in celery, aster, and periwinkle plants and in Macrosteles severini leafhopper vectors infected with either the dwarf AY-MLO, Tulelake AY-MLO, or SAY-MLO strain. Plasmids were also detected in two California field isolates of AY-MLO but not in plants infected with the beet leafhopper-transmitted virescence agent, western X, or elm yellows MLOs. SAY-MLO plasmids were 5.2, 4.9, 3.4, and 1.7 kilobase pairs in size. Plasmids isolated from dwarf AY- and Tulelake AY-MLOs were 7.4, 5.1, 3.5, and 1.7 kilobase pairs in size. No evidence was obtained for integration of SAY-MLO plasmids into the MLO chromosome.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kuske CR,Kirkpatrick BC

doi

10.1128/jb.172.3.1628-1633.1990

subject

Has Abstract

pub_date

1990-03-01 00:00:00

pages

1628-33

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

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