Characterization of a Staphylococcus aureus bacteriocin.

Abstract:

:The bacteriocin produced by a strain of Staphylococcus aureus has been isolated and designated staphylococcin (414), and a study was made of its chemical, physical, and biological properties. The staphylococcin is released in appreciable quantities after breakage of the cells and can be purified through differential centrifugation and column chromatography. In the native state, it appears to be a lipoprotein-carbohydrate complex with a molecular weight in excess of 200,000. The complex can be dissociated by sodium dodecyl sulfate into smaller subunits which retain activity. The gross chemical and physical properties of the bacteriocin closely resemble those ascribed to certain preparations of cell membranes. Staphylococcin (414) is not a lytic enzyme like lysostaphin and does not have the same spectrum of activity. Like other bacteriocins from gram-positive microorganisms, it does not inhibit any gram-negative bacteria, but does inhibit several other genera.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gagliano VJ,Hinsdill RD

doi

10.1128/JB.104.1.117-125.1970

subject

Has Abstract

pub_date

1970-10-01 00:00:00

pages

117-25

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

104

pub_type

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