Fine structure of Vibrio cholerae during toxin production.

Abstract:

:The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope permeability, is evident particularly during the late logarithmic and early stationary phases of growth. Certain extracellular material demonstrable with negative-stain techniques does appear during the period of toxin production. The possible origin of this material is discussed. The effects of high temperature (37 C) and aeration on cell structure are also examined.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kennedy JR,Richardson SH

doi

10.1128/JB.100.3.1393-1401.1969

subject

Has Abstract

pub_date

1969-12-01 00:00:00

pages

1393-401

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

100

pub_type

杂志文章
  • Identification of a target gene and activating stimulus for the YpdA/YpdB histidine kinase/response regulator system in Escherichia coli.

    abstract::Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02051-12

    authors: Fried L,Behr S,Jung K

    更新日期:2013-02-01 00:00:00

  • Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence.

    abstract::Virulence in Shigella spp., as well as in strains of enteroinvasive Escherichia coli, is regulated by growth temperature. Previously, virR had been identified as the gene controlling the temperature-regulated expression of Shigella virulence. Since Shigella spp. and E. coli are also known to share greater than 90% DNA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2879-2881.1989

    authors: Hromockyj AE,Maurelli AT

    更新日期:1989-05-01 00:00:00

  • Permeability of bacterial spores. II. Molecular variables affecting solute permeation.

    abstract::Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750-760. 1961.-More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.5.750-760.1961

    authors: GERHARDT P,BLACK SH

    更新日期:1961-11-01 00:00:00

  • Characterization and functional analysis of atl, a novel gene encoding autolysin in Streptococcus suis.

    abstract::Streptococcus suis serotype 2 (S. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. A novel gene, designated atl and encoding a major autolysin of S. suis 2 virulent strain HA9801, was identified and characterized in this study. The Atl protein contains 1,025 amino acids with ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06231-11

    authors: Ju CX,Gu HW,Lu CP

    更新日期:2012-03-01 00:00:00

  • Identification of the PhoB Regulon and Role of PhoU in the Phosphate Starvation Response of Caulobacter crescentus.

    abstract:UNLABELLED:An ability to sense and respond to changes in extracellular phosphate is critical for the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitation throug...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00658-15

    authors: Lubin EA,Henry JT,Fiebig A,Crosson S,Laub MT

    更新日期:2015-10-19 00:00:00

  • Contribution of the FtsQ transmembrane segment to localization to the cell division site.

    abstract::The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00723-07

    authors: Scheffers DJ,Robichon C,Haan GJ,den Blaauwen T,Koningstein G,van Bloois E,Beckwith J,Luirink J

    更新日期:2007-10-01 00:00:00

  • Plasmid deoxyribonucleic acid replication in Streptomyces griseus.

    abstract::A series of electron micrographs showing the presence of different molecular forms representing various replication stages of plasmid deoxyribonucleic acid from Streptomyces griseus was obtained. Based upon an analysis of these electron micrographs, a tentative model for plasmid deoxyribonucleic acid replication in S....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.1.412-414.1981

    authors: Xue Y,Zhuang Z,Zhu Y,Xu Y,Dong K

    更新日期:1981-04-01 00:00:00

  • A second branched-chain alpha-keto acid dehydrogenase gene cluster (bkdFGH) from Streptomyces avermitilis: its relationship to avermectin biosynthesis and the construction of a bkdF mutant suitable for the production of novel antiparasitic avermectins.

    abstract::A second cluster of genes encoding the E1 alpha, E1 beta, and E2 subunits of branched-chain alpha-keto acid dehydrogenase (BCDH), bkdFGH, has been cloned and characterized from Streptomyces avermitilis, the soil microorganism which produces anthelmintic avermectins. Open reading frame 1 (ORF1) (bkdF, encoding E1 alpha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.12.3504-3511.1995

    authors: Denoya CD,Fedechko RW,Hafner EW,McArthur HA,Morgenstern MR,Skinner DD,Stutzman-Engwall K,Wax RG,Wernau WC

    更新日期:1995-06-01 00:00:00

  • Methylation-Induced Hypermutation in Natural Populations of Bacteria.

    abstract::Methylation of DNA at the C-5 position of cytosine occurs in diverse organisms. This modification can increase the rate of C→T transitions at the methylated position. In Escherichia coli and related enteric bacteria, the inner C residues of the sequence CCWGG (W is A or T) are methylated by the Dcm enzyme. These sites...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00371-18

    authors: Cherry JL

    更新日期:2018-11-26 00:00:00

  • Kinetics and catalytic properties of coenzyme A transferase from Peptostreptococcus elsdenii.

    abstract::Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,00...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.1.372-381.1976

    authors: Schulman M,Valentino D

    更新日期:1976-10-01 00:00:00

  • Regulation of expression of the Escherichia coli K-12 mtr gene by TyrR protein and Trp repressor.

    abstract::The Escherichia coli K-12 mtr gene, which encodes a tryptophan-specific permease, was cloned, and its nucleotide sequence was determined. The precise location of the mtr gene at 69 min on the E. coli chromosome was determined. The mtr gene product was identified as a 414-amino-acid residue protein with a calculated mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.13.4133-4143.1991

    authors: Sarsero JP,Wookey PJ,Pittard AJ

    更新日期:1991-07-01 00:00:00

  • Conservative duplication of spindle poles during meiosis in Saccharomyces cerevisiae.

    abstract::During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.7.2372-2375.2001

    authors: Wesp A,Prinz S,Fink GR

    更新日期:2001-04-01 00:00:00

  • Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli.

    abstract::The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene enco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.12.5343-5352.1987

    authors: Griggs DW,Tharp BB,Konisky J

    更新日期:1987-12-01 00:00:00

  • Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain.

    abstract::The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00476-07

    authors: Maul RW,Sanders LH,Lim JB,Benitez R,Sutton MD

    更新日期:2007-07-01 00:00:00

  • Localization of the cis-acting regulatory DNA sequences of the Myxococcus xanthus tps and ops genes.

    abstract::The cis-acting regulatory regions of the tps and ops genes of Myxococcus xanthus were localized by analyzing the expression of fusions of these genes with the lacZ gene. A 201-base-pair (bp) fragment of tps DNa extending 95 bp upstream (-95) from the transcriptional start was sufficient to direct developmentally regul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4931-4938.1988

    authors: Downard JS,Kim SH,Kil KS

    更新日期:1988-10-01 00:00:00

  • Numbers of individual nutrient germinant receptors and other germination proteins in spores of Bacillus subtilis.

    abstract::Germination of dormant Bacillus subtilis spores with specific nutrient germinants is dependent on a number of inner membrane (IM) proteins, including (i) the GerA, GerB, and GerK germinant receptors (GRs) that respond to nutrient germinants; (ii) the GerD protein, essential for optimal GR function; and (iii) SpoVA pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00377-13

    authors: Stewart KA,Setlow P

    更新日期:2013-08-01 00:00:00

  • Role of Sphingomonas sp. strain Fr1 PhyR-NepR-σEcfG cascade in general stress response and identification of a negative regulator of PhyR.

    abstract::The general stress response in Alphaproteobacteria was recently described to depend on the alternative sigma factor σ(EcfG), whose activity is regulated by its anti-sigma factor NepR. The response regulator PhyR, in turn, regulates NepR activity in a partner-switching mechanism according to which phosphorylation of Ph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06006-11

    authors: Kaczmarczyk A,Campagne S,Danza F,Metzger LC,Vorholt JA,Francez-Charlot A

    更新日期:2011-12-01 00:00:00

  • Isolation, identification, and structural analysis of the mycobactins of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, and Mycobacterium paratuberculosis.

    abstract::Methods were devised to purify the cell-associated, iron-binding compounds known as mycobactins from the closely related species Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (i.e., the MAIS complex of organisms). The mycobactins from these three species showed a structure that is c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.896-903.1985

    authors: Barclay R,Ewing DF,Ratledge C

    更新日期:1985-11-01 00:00:00

  • Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes.

    abstract::The DNA-binding factor BifA (previously called VF1) binds upstream of the developmentally regulated site-specific recombinase gene xisA in the cyanobacterium Anabaena sp. strain PCC 7120. Besides binding xisA, BifA also binds the glnA, rbcL, and nifH promoter regions. DNase I footprint analysis of BifA binding to glnA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1214-1223.1994

    authors: Ramasubramanian TS,Wei TF,Golden JW

    更新日期:1994-03-01 00:00:00

  • Discontinuity and limited linkage in the homologous recombination system of a hyperthermophilic archaeon.

    abstract::Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00447-10

    authors: Grogan DW,Rockwood J

    更新日期:2010-09-01 00:00:00

  • Mutational analysis of cat-86 gene expression controlled by lactococcal promoters in Lactococcus lactis subsp. lactis and Escherichia coli.

    abstract::Promoters were cloned from the chromosomal DNA of Lactococcus lactis subsp. lactis NP4510 by using promoter-probe vector pGKV210. N-Methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis of L. lactis-(pBV413), with low-level expression of the cat-86 gene, resulted in enhanced expression. Subcloning and sequencing of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.21.6754-6758.1994

    authors: Bojovic B,Djordjevic G,Banina A,Topisirovic L

    更新日期:1994-11-01 00:00:00

  • Proton pump coupled to cytochrome c oxidase in the cyanobacterium Anacystis nidulans.

    abstract::Intact spheroplasts of the cyanobacterium Anacystis nidulans were found to oxidize various exogenous c-type cytochromes with concomitant proton extrusion. In the coupled state, H+/e stoichiometries close to 1 were measured, regardless of absolute reaction rates. It is concluded that the proton translocation observed i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.1.539-542.1983

    authors: Peschek GA

    更新日期:1983-01-01 00:00:00

  • Chemotaxis in Pseudomonas aeruginosa.

    abstract::A chemotaxis system for Pseudomonas aeruginosa was defined by using the method of Adler. Cells were attracted to compounds in the order ammonium chloride greater than amino acids greater than organic acids. Two sugars were assayed and elicited no response. Comparisons with other model systems are discussed. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.1.427-429.1978

    authors: Moench TT,Konetzka WA

    更新日期:1978-01-01 00:00:00

  • Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I.

    abstract::Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.2.676-681.1972

    authors: Shizuya H,Dykhuizen D

    更新日期:1972-11-01 00:00:00

  • Control of photosynthetic membrane assembly in Rhodobacter sphaeroides mediated by puhA and flanking sequences.

    abstract::A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.436-446.1989

    authors: Sockett RE,Donohue TJ,Varga AR,Kaplan S

    更新日期:1989-01-01 00:00:00

  • Comparative studies of lipoteichoic acids from several Bacillus strains.

    abstract::Structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 Bacillus strains by phenol-water partition followed by chromatography on DEAE-Sephacel and Octyl-Sepharose columns. A group of the tested bacteria (group A), Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus, was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.508-516.1986

    authors: Iwasaki H,Shimada A,Ito E

    更新日期:1986-08-01 00:00:00

  • Plasmid-to-chromosome gene transfer in Haemophilus influenza during growth.

    abstract::A low level of recombination between homologous regions of plasmids and the host chromosome occurred during cell growth. The plasmids contained antibiotic resistance markers on the homologous regions which were only expressed when they were integrated into the chromosome. Such recombination took place in Rec+ rec-2 mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.1.458-459.1986

    authors: Balganesh M,Arrigoni L,Setlow JK

    更新日期:1986-10-01 00:00:00

  • Candida albicans secreted aspartyl proteinases: isoenzyme pattern is determined by cell type, and levels are determined by environmental factors.

    abstract::For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.18.5215-5221.1995

    authors: White TC,Agabian N

    更新日期:1995-09-01 00:00:00

  • Modification of peptidoglycan precursors is a common feature of the low-level vancomycin-resistant VANB-type Enterococcus D366 and of the naturally glycopeptide-resistant species Lactobacillus casei, Pediococcus pentosaceus, Leuconostoc mesenteroides, and

    abstract::The biochemical basis for the acquired or natural resistance of various gram-positive organisms to glycopeptides was studied by high-pressure liquid chromatographic analysis of their peptidoglycan UDP-MurNAc-peptide precursors. In all cases, resistance was correlated with partial or complete replacement of the C-termi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.8.2398-2405.1994

    authors: Billot-Klein D,Gutmann L,Sablé S,Guittet E,van Heijenoort J

    更新日期:1994-04-01 00:00:00

  • Ultrastructal morphology of some prokaryotic microorganisms associated with the hindgut of cockroaches.

    abstract::Scanning electron microscopy and transmission electron microscopy have been used to visualize the morphology and ultrastructure of two types of microorganisms in the hindgut of the cockroach Blaberus posticus. Both organisms, designated as either short or long rods, are attached to chitinous projections from the gut w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.336-345.1975

    authors: Foglesong MA,Walker DH Jr,Puffer JS,Markovetz AJ

    更新日期:1975-07-01 00:00:00