Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence.


:Virulence in Shigella spp., as well as in strains of enteroinvasive Escherichia coli, is regulated by growth temperature. Previously, virR had been identified as the gene controlling the temperature-regulated expression of Shigella virulence. Since Shigella spp. and E. coli are also known to share greater than 90% DNA sequence homology, we sought to determine if nonpathogenic E. coli K-12 C600 contains a gene homologous to the Shigella flexneri 2a gene virR. Through the use of transduction and molecular cloning of strain C600 chromosomal DNA we have shown that E. coli K-12 does indeed contain a gene functionally homologous to the virR of S. flexneri.


J Bacteriol


Journal of bacteriology


Hromockyj AE,Maurelli AT




Has Abstract


1989-05-01 00:00:00












  • Cloning and expression in Escherichia coli of the dnaK gene of Zymomonas mobilis.

    abstract::The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Michel GP

    更新日期:1993-05-01 00:00:00

  • Role of the Eikenella corrodens pilA locus in pilus function and phase variation.

    abstract::The human pathogen Eikenella corrodens expresses type IV pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. On solid medium, piliated variants form small (S-phase), corroding colonies whereas nonpiliated variants form large (L-phase), noncorroding colonies....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Villar MT,Hirschberg RL,Schaefer MR

    更新日期:2001-01-01 00:00:00

  • Interaction between two regulatory proteins in osmoregulatory expression of ompF and ompC genes in Escherichia coli: a novel ompR mutation suppresses pleiotropic defects caused by an envZ mutation.

    abstract::The ompR and envZ genes, which together constitute the ompB operon, are involved in osmoregulatory expression of the OmpF and OmpC proteins, major outer membrane proteins of Escherichia coli. The envZ11 mutation results in the OmpF- OmpC-constitutive phenotype. A mutant which suppressed defects caused by the envZ11 mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Matsuyama S,Mizuno T,Mizushima S

    更新日期:1986-12-01 00:00:00

  • The Rhizobium etli cyaC product: characterization of a novel adenylate cyclase class.

    abstract::Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Téllez-Sosa J,Soberón N,Vega-Segura A,Torres-Márquez ME,Cevallos MA

    更新日期:2002-07-01 00:00:00

  • Purification and characterization of the repressor of the shiga toxin-encoding bacteriophage 933W: DNA binding, gene regulation, and autocleavage.

    abstract::The genes encoding Shiga toxin (stx), the major virulence factor of Shiga toxin-encoding Escherichia coli (STEC) strains, are carried on lambdoid prophages resident in all known STEC strains. The stx genes are expressed only during lytic growth of these temperate bacteriophages. We cloned the gene encoding the repress...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Koudelka AP,Hufnagel LA,Koudelka GB

    更新日期:2004-11-01 00:00:00

  • Integration of rotation and piston motions in coiled-coil signal transduction.

    abstract::A coordinated response to a complex and dynamic environment requires an organism to simultaneously monitor and interpret multiple signaling cues. In bacteria and some eukaryotes, environmental responses depend on the histidine autokinases (HKs). For example, VirA, a large integral membrane HK from Agrobacterium tumefa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gao R,Lynn DG

    更新日期:2007-08-01 00:00:00

  • Isocitrate dehydrogenase kinase/phosphatase: aceK alleles that express kinase but not phosphatase activity.

    abstract::For Escherichia coli, growth on acetate requires the induction of the enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase. The branch point between the glyoxylate bypass and the Krebs cycle is controlled by phosphorylation of isocitrate dehydrogenase (IDH), inhibiting that enzyme's activity and thus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ikeda T,LaPorte DC

    更新日期:1991-03-01 00:00:00

  • Negative regulator of sigma G-controlled gene expression in stationary-phase Bacillus subtilis.

    abstract::In some media, Bacillus subtilis can maintain a prolonged stationary growth phase; however, in other media, nutrient depletion triggers a complex differentiation that culminates in production of a dormant endospore. This differentiation requires the expression of many genes. We found that during the stationary phase i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rather PN,Coppolecchia R,DeGrazia H,Moran CP Jr

    更新日期:1990-02-01 00:00:00

  • Genetic and physical analyses of Caulobacter crescentus trp genes.

    abstract::Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Winkler ME,Schoenlein PV,Ross CM,Barrett JT,Ely B

    更新日期:1984-10-01 00:00:00

  • Localization of acyl carrier protein in Escherichia coli.

    abstract::Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jackowski S,Edwards HH,Davis D,Rock CO

    更新日期:1985-04-01 00:00:00

  • Annotated genome sequence of Lactobacillus pentosus MP-10, which has probiotic potential, from naturally fermented Aloreña green table olives.

    abstract::Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Abriouel H,Benomar N,Pulido RP,Cañamero MM,Gálvez A

    更新日期:2011-09-01 00:00:00

  • Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila.

    abstract::Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jablonski PE,Ferry JG

    更新日期:1991-04-01 00:00:00

  • Gut Microbiota: the Emerging Link to Lung Homeostasis and Disease.

    abstract::The gut microbiota plays a crucial role in the development of the immune system and confers benefits or disease susceptibility to the host. Emerging studies have indicated the gut microbiota could affect pulmonary health and disease through cross talk between the gut microbiota and the lungs. Gut microbiota dysbiosis c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审


    authors: Zhou A,Lei Y,Tang L,Hu S,Yang M,Wu L,Yang S,Tang B

    更新日期:2021-01-25 00:00:00

  • Genome-wide transcriptional analysis of the phosphate starvation stimulon of Bacillus subtilis.

    abstract::Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the acquisition of phosphate (P(i)) from the environment and reducing the cellular requirement for this essential nutrient, th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Allenby NE,O'Connor N,Prágai Z,Ward AC,Wipat A,Harwood CR

    更新日期:2005-12-01 00:00:00

  • Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.

    abstract::A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Berg CM,Liu L,Wang B,Wang MD

    更新日期:1988-01-01 00:00:00

  • Mutational analysis of the Myxococcus xanthus Omicron4499 promoter region reveals shared and unique properties in comparison with other C-signal-dependent promoters.

    abstract::The bacterium Myxococcus xanthus undergoes multicellular development during times of nutritional stress and uses extracellular signals to coordinate cell behavior. C-signal affects gene expression late in development, including that of Omega4499, an operon identified by insertion of Tn5 lac into the M. xanthus chromos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yoder DR,Kroos L

    更新日期:2004-06-01 00:00:00

  • Complete genome sequence of the strong mutator Salmonella enterica subsp. enterica serotype Heidelberg strain B182.

    abstract::In bacteria, normal mutation frequencies are mostly around 10(-10) per base pair. However, there exists natural isolates, called "mutators," that exhibit permanent mutation occurrences up to 1,000-fold greater than usual. As mutations play essential roles, particularly in the evolution of antibiotic resistance, bacter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Le Bars H,Bousarghin L,Bonnaure-Mallet M,Jolivet-Gougeon A,Barloy-Hubler F

    更新日期:2012-07-01 00:00:00

  • Identification of a Cytopathogenic Toxin from Sneathia amnii.

    abstract::Sneathia amnii is a poorly characterized emerging pathogen that has been implicated in amnionitis and urethritis. We found that S. amnii damages fetal membranes, and we identified and purified a cytotoxic exotoxin that lyses human red blood cells and damages cells from fetal membranes. The gene appears to be cotranscr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gentile GL,Rupert AS,Carrasco LI,Garcia EM,Kumar NG,Walsh SW,Jefferson KK

    更新日期:2020-06-09 00:00:00

  • Mutations in the -10 TATAAT sequence of the gyrA promoter affect both promoter strength and sensitivity to DNA supercoiling.

    abstract::Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Straney R,Krah R,Menzel R

    更新日期:1994-10-01 00:00:00

  • Molecular nature of spontaneous modifications in gacS which cause colony phase variation in Pseudomonas sp. strain PCL1171.

    abstract::Pseudomonas sp. strain PCL1171 displays colony phase variation between opaque phase I and translucent phase II colonies, thereby regulating the production of secondary metabolites and exoenzymes. Complementation and sequence analysis of 26 phase II mutants and of 13 wild-type phase II sectors growing out of phase I co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: van den Broek D,Chin-A-Woeng TF,Bloemberg GV,Lugtenberg BJ

    更新日期:2005-01-01 00:00:00

  • icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in Staphylococcus epidermidis.

    abstract::Biofilm formation in Staphylococcus epidermidis is dependent upon the ica operon-encoded polysaccharide intercellular adhesin, which is subject to phase-variable and environmental regulation. The icaR gene, located adjacent to the ica operon, appears to be a member of the tetR family of transcriptional regulators. In ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Conlon KM,Humphreys H,O'Gara JP

    更新日期:2002-08-01 00:00:00

  • A truncated form of the Pho80 cyclin of Saccharomyces cerevisiae induces expression of a small cytosolic factor which inhibits vacuole inheritance.

    abstract::Vacuoles project streams of vesicles and membranous tubules into the yeast bud where they fuse, founding the daughter cell organelle, vac5-1, which encodes a truncated form of the Pho80 cyclin, inhibits normal vacuole inheritance. An in vitro inheritance assay which measures the fusion of vacuoles serves as a model fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nicolson T,Conradt B,Wickner W

    更新日期:1996-07-01 00:00:00

  • Characterization of an A-factor-responsive repressor for amfR essential for onset of aerial mycelium formation in Streptomyces griseus.

    abstract::A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

    更新日期:1998-10-01 00:00:00

  • Molecular characterization of "plasmid-free" antibiotic-resistant Haemophilus influenzae.

    abstract::We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Roberts MC,Smith AL

    更新日期:1980-10-01 00:00:00

  • Acid-soluble spore proteins of Bacillus subtilis.

    abstract::Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Johnson WC,Tipper DJ

    更新日期:1981-06-01 00:00:00

  • Electromechanical interactions in cell walls of gram-positive cocci.

    abstract::Isolated cell walls of Staphylococcus aureus and Micrococcus lysodeikticus were found to expand and contract in response to changes in environmental pH and ionic strength. These volume changes, which could amount to as much as a doubling of wall dextran-impermeable volume, were related to changes in electrostatic inte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ou LT,Marquis RE

    更新日期:1970-01-01 00:00:00

  • The Pseudomonas aeruginosa algC gene encodes phosphoglucomutase, required for the synthesis of a complete lipopolysaccharide core.

    abstract::We have constructed strains of Pseudomonas aeruginosa with mutations in the algC gene, previously shown to encode the enzyme phosphomannomutase. The algC mutants of a serotype O5 strain (PAO1) and a serotype O3 strain (PAC1R) did not express lipopolysaccharide (LPS) O side chains or the A-band (common antigen) polysac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Coyne MJ Jr,Russell KS,Coyle CL,Goldberg JB

    更新日期:1994-06-01 00:00:00

  • Regulation of a common amidotransferase subunit.

    abstract::In Bacillus subtilis the trpX locus specifies a glutamine-binding protein designated subunit X, which forms a complex with subunit E to constitute the anthranilate synthase enzyme aggregate (EX) and subunit A to constitute the p-aminobenzoate synthase enzyme aggregate (AX). Subunit X confers upon these enzyme complexe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kane JF

    更新日期:1977-11-01 00:00:00

  • Dynamics of the SetCD-regulated integration and excision of genomic islands mobilized by integrating conjugative elements of the SXT/R391 family.

    abstract::Mobilizable genomic islands (MGIs) are small genomic islands that are mobilizable by SXT/R391 integrating conjugative elements (ICEs) due to similar origins of transfer. Their site-specific integration and excision are catalyzed by the integrase that they encode, but their conjugative transfer entirely depends upon th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Daccord A,Mursell M,Poulin-Laprade D,Burrus V

    更新日期:2012-11-01 00:00:00

  • Properties of Bacillus megaterium temperature-sensitive germination mutants.

    abstract::Bacillus megaterium mutants JV-9 and JV-10 are temperature sensitive for initiation of spore germination. At 46 C, they did not lose heat resistance, dipicolinic acid, or absorbance, indicating that the temperature-sensitive blocks are very early in the sequence of initiation reactions. Strain JV-9 was temperature sen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vary JC

    更新日期:1975-01-01 00:00:00