Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence.


:Virulence in Shigella spp., as well as in strains of enteroinvasive Escherichia coli, is regulated by growth temperature. Previously, virR had been identified as the gene controlling the temperature-regulated expression of Shigella virulence. Since Shigella spp. and E. coli are also known to share greater than 90% DNA sequence homology, we sought to determine if nonpathogenic E. coli K-12 C600 contains a gene homologous to the Shigella flexneri 2a gene virR. Through the use of transduction and molecular cloning of strain C600 chromosomal DNA we have shown that E. coli K-12 does indeed contain a gene functionally homologous to the virR of S. flexneri.


J Bacteriol


Journal of bacteriology


Hromockyj AE,Maurelli AT




Has Abstract


1989-05-01 00:00:00












  • Regulation of lysine- and lysine-plus-threonine-inhibitable aspartokinases in Bacillus brevis.

    abstract::Further studies on the expression of the two aspartokinase activities in Bacillus bovis are presented. Aspartokinase I (previously shown to be inhibited and repressed by lysine) was found to be repressed by diaminopimelate in the wild-type strain. However, in a mutant unable to convert diaminopimelate to lysine, starv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hitchcock MJ,Hodgson B,Linforth JL

    更新日期:1980-05-01 00:00:00

  • Cloning of Escherichia coli and Pseudomonas aeruginosa phosphomannose isomerase genes and their expression in alginate-negative mutants of Pseudomonas aeruginosa.

    abstract::The phosphomannose isomerase (pmi) gene of Escherichia coli was cloned on a broad-host-range cosmid vector and expressed in Pseudomonas aeruginosa at a low level. Plasmid pAD3, which harbors the E. coli pmi gene, contains a 6.2-kilobase-pair HindIII fragment derived from the chromosome of E. coli. Subcloning produced ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Darzins A,Nixon LL,Vanags RI,Chakrabarty AM

    更新日期:1985-01-01 00:00:00

  • An extraintestinal, pathogenic isolate of Escherichia coli (O4/K54/H5) can produce a group 1 capsule which is divergently regulated from its constitutively produced group 2, K54 capsular polysaccharide.

    abstract::We are studying an O4/K54/H5 Escherichia coli bacteremic isolate (CP9) as a model pathogen for extraintestinal infection. Its group 2, K54 capsular polysaccharide is an important virulence determinant and confers serum resistance. In this study the effect of the group 1 capsule regulators, RcsA, RcsB, and Lon protease...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Russo TA,Singh G

    更新日期:1993-12-01 00:00:00

  • Isolation and analysis of the Xanthomonas alkyl hydroperoxide reductase gene and the peroxide sensor regulator genes ahpC and ahpF-oxyR-orfX.

    abstract::From Xanthomonas campestris pv. phaseoli, we have isolated by two independent methods genes involved in peroxide detoxification (ahpC and ahpF), a gene involved in peroxide sensing and transcription regulation (oxyR), and a gene of unknown function (orfX). Amino acid sequence analysis of AhpC, AhpF, and OxyR showed hi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Loprasert S,Atichartpongkun S,Whangsuk W,Mongkolsuk S

    更新日期:1997-06-01 00:00:00

  • Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae.

    abstract::Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McCullough J,Herskowitz I

    更新日期:1979-04-01 00:00:00

  • Amplification of bacterial genomic DNA by the polymerase chain reaction and direct sequencing after asymmetric amplification: application to the study of periplasmic permeases.

    abstract::The polymerase chain reaction (PCR) has been used to amplify DNA fragments by using eucaryotic genomic DNA as a template. We show that bacterial genomic DNA can be used as a template for PCR amplification. We demonstrate that DNA fragments at least as large as 4,400 base pairs can be amplified with fidelity and that t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shyamala V,Ames GF

    更新日期:1989-03-01 00:00:00

  • Dihydrofolate reductase activity in strains of Streptococcus faecium var. durans resistant to methasquin and amethopterin.

    abstract::Resistance to the antifolates methasquin and amethopterin has been studied in new strains of Streptococcus faecium var. durans. Two methasquin-resistant strains (SF/MQ, SF/MQ(T)) and an amethopterin-resistant strain (SF/AM) were selected independently from the wild-type S. faecium var. durans (SF/O). SF/MQ(T) is a thy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rader JI,Hutchison DJ

    更新日期:1972-04-01 00:00:00

  • Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea.

    abstract::Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Franzetti B,Sohlberg B,Zaccai G,von Gabain A

    更新日期:1997-02-01 00:00:00

  • Factors regulating competence in transformation of streptococci.

    abstract::Pakula, Roman (University of Toronto, Toronto, Ontario, Canada). Factors regulating competence in transformation of streptococci. J. Bacteriol. 90:1320-1324. 1965.-The highly transformable group H Streptococcus strain Challis produced an exocellular competence-provoking enzyme capable of converting to the state of com...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pakula R

    更新日期:1965-11-01 00:00:00

  • Organization and regulation of pentachlorophenol-degrading genes in Sphingobium chlorophenolicum ATCC 39723.

    abstract::The first three enzymes of the pentachlorophenol (PCP) degradation pathway in Sphingobium chlorophenolicum (formerly Sphingomonas chlorophenolica) ATCC 39723 have been characterized, and the corresponding genes, pcpA, pcpB, and pcpC, have been individually cloned and sequenced. To search for new genes involved in PCP ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cai M,Xun L

    更新日期:2002-09-01 00:00:00

  • Localization of the Escherichia coli rnt gene encoding RNase T by using a combination of physical and genetic mapping.

    abstract::The rnt gene encoding RNase T was cloned on a 13-kilobase BamHI fragment. Restriction analysis of the fragment and comparison of it with the Escherichia coli restriction map localized rnt to kilobase coordinates 1733 to 1746, corresponding to about 36 min on the genetic map. The map location was confirmed by cotransdu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Case LM,Chen XN,Deutscher MP

    更新日期:1989-10-01 00:00:00

  • Escherichia coli Lrp (leucine-responsive regulatory protein) does not directly regulate expression of the leu operon promoter.

    abstract::Studies by R. Lin et al. (J. Bacteriol. 174:1948-1955, 1992) suggested that the Escherichia coli leu operon might be a member of the Lrp regulon. Their results were obtained with a leucine auxotroph; in leucine prototrophs grown in a medium lacking leucine, there was little difference in leu operon expression between ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Landgraf JR,Boxer JA,Calvo JM

    更新日期:1999-10-01 00:00:00

  • ToxR proteins with substitutions in residues conserved with OmpR fail to activate transcription from the cholera toxin promoter.

    abstract::The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ottemann KM,DiRita VJ,Mekalanos JJ

    更新日期:1992-11-01 00:00:00

  • Genome sequence of Rickettsia conorii subsp. israelensis, the agent of Israeli spotted fever.

    abstract::Rickettsia conorii subsp. israelensis is the agent of Israeli spotted fever. The present study reports the draft genome of Rickettsia conorii subsp. israelensis strain ISTT CDC1, isolated from a Rhipicephalus sanguineus tick collected in Israel. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sentausa E,El Karkouri K,Robert C,Raoult D,Fournier PE

    更新日期:2012-09-01 00:00:00

  • A New Essential Cell Division Protein in Caulobacter crescentus.

    abstract::Bacterial cell division is a complex process that relies on a multiprotein complex composed of a core of widely conserved and generally essential proteins and on accessory proteins that vary in number and identity in different bacteria. The assembly of this complex and, particularly, the initiation of constriction are...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Osorio A,Camarena L,Cevallos MA,Poggio S

    更新日期:2017-03-28 00:00:00

  • Rhizobium meliloti mutants unable to synthesize anthranilate display a novel symbiotic phenotype.

    abstract::Analyses of Rhizobium meliloti trp auxotrophs suggest that anthranilate biosynthesis by the R. meliloti trpE(G) gene product is necessary during nodule development for establishment of an effective symbiosis. trpE(G) mutants, as well as mutants blocked earlier along this pathway in aromatic amino acid biosynthesis, fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Barsomian GD,Urzainqui A,Lohman K,Walker GC

    更新日期:1992-07-01 00:00:00

  • Aromatic acid metabolites of Escherichia coli K-12 can induce the marRAB operon.

    abstract::MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We al...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chubiz LM,Rao CV

    更新日期:2010-09-01 00:00:00

  • Vibrio cholerae VciB promotes iron uptake via ferrous iron transporters.

    abstract::Vibrio cholerae uses a variety of strategies for obtaining iron in its diverse environments. In this study we report the identification of a novel iron utilization protein in V. cholerae, VciB. The vciB gene and its linked gene, vciA, were isolated in a screen for V. cholerae genes that permitted growth of an Escheric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mey AR,Wyckoff EE,Hoover LA,Fisher CR,Payne SM

    更新日期:2008-09-01 00:00:00

  • Iron-dependent transcription of the frpB gene of Helicobacter pylori is controlled by the Fur repressor protein.

    abstract::We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpB and ceuE) and oxygen radical detoxification (katA and tsaA). DNase I footprint analysis showed that Fur binds specifically to a high-affinity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Delany I,Pacheco AB,Spohn G,Rappuoli R,Scarlato V

    更新日期:2001-08-01 00:00:00

  • Amikacin resistance mediated by multiresistance transposon Tn2424.

    abstract::Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Meyer JF,Nies BA,Wiedemann B

    更新日期:1983-08-01 00:00:00

  • Molecular characterization of "plasmid-free" antibiotic-resistant Haemophilus influenzae.

    abstract::We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Roberts MC,Smith AL

    更新日期:1980-10-01 00:00:00

  • The gene encoding the glutamate dehydrogenase in Lactococcus lactis is part of a remnant Tn3 transposon carried by a large plasmid.

    abstract::The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tanous C,Chambellon E,Sepulchre AM,Yvon M

    更新日期:2005-07-01 00:00:00

  • Linker mutagenesis of the Caulobacter crescentus S-layer protein: toward a definition of an N-terminal anchoring region and a C-terminal secretion signal and the potential for heterologous protein secretion.

    abstract::Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bingle WH,Nomellini JF,Smit J

    更新日期:1997-02-01 00:00:00

  • Genome sequence of a dominant, multidrug-resistant Acinetobacter baumannii strain, TCDC-AB0715.

    abstract::Acinetobacter baumannii has emerged as a significant nosocomial pathogen worldwide. The increasing trend of carbapenem and fluoroquinolone resistance in A. baumannii severely limits the usage of therapeutic antimicrobial agents. Here we report the genome sequence of a multidrug-resistant A. baumannii strain, TCDC-AB07...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chen CC,Lin YC,Sheng WH,Chen YC,Chang SC,Hsia KC,Liao MH,Li SY

    更新日期:2011-05-01 00:00:00

  • Isolation of Neurospora crassa bradytrophs.

    abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kinsey JA

    更新日期:1979-12-01 00:00:00

  • Overexpression of LolCDE allows deletion of the Escherichia coli gene encoding apolipoprotein N-acyltransferase.

    abstract::Bacterial lipoproteins represent a subset of membrane-associated proteins that are covalently modified with lipids at the N-terminal cysteine. The final step of lipoprotein modification, N-acylation of apolipoproteins, is mediated by apolipoprotein N-acyltransferase (Lnt). Examinations with reconstituted proteoliposom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Narita S,Tokuda H

    更新日期:2011-09-01 00:00:00

  • Transcriptional regulation of the phosphotransacetylase-encoding and acetate kinase-encoding genes (pta and ack) from Methanosarcina thermophila.

    abstract::Phosphotransacetylase and acetate kinase catalyze the activation of acetate to acetyl coenzyme A in the first step of methanogenesis from acetate in Methanosarcina thermophila. The genes encoding these enzymes (pta and ack) have been cloned and sequenced. They are arranged on the chromosome with pta upstream of ack (M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Singh-Wissmann K,Ferry JG

    更新日期:1995-04-01 00:00:00

  • Improvement in K+-limited growth rate associated with expression of the N-terminal fragment of one subunit (KdpA) of the multisubunit Kdp transporter in Escherichia coli.

    abstract::Mutations in any one of three genes, kdpA, -B, or -C, in Escherichia coli abolish the activity of Kdp, a multisubunit K+-ATPase that belongs to the P-type ATPase family of cation transporters. We found in this study that expression in vivo of a 135-amino-acid-long N-terminal fragment (KdpA'), less than one-quarter the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sardesai AA,Gowrishankar J

    更新日期:2001-06-01 00:00:00

  • Metabolism of biphenyl by Aspergillus toxicarius: induction of hydroxylating activity and accumulation of water-soluble conjugates.

    abstract::Biphenyl metabolism in Aspergillus toxicarius occurs by successive hydroxylations in the 4- and 4'-positions, followed by conjugation with sulfate to produce 4-hydroxybiphenyl-O-sulfonic acid and 4,4'-dihydroxybiphenyl-O-sulfonic acid. The hydroxylation reactions normally occur only after a prolonged lag period after ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Golbeck JH,Albaugh SA,Radmer R

    更新日期:1983-10-01 00:00:00

  • (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase, a product of the mva operon of Pseudomonas mevalonii, is regulated at the transcriptional level.

    abstract::We have cloned and sequenced a 505-base-pair (bp) segment of DNA situated upstream of mvaA, the structural gene for (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase (EC of Pseudomonas mevalonii. The DNA segment that we characterized includes the promoter region for the mva operon. Nuclease S1 mapping and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang YL,Beach MJ,Rodwell VW

    更新日期:1989-10-01 00:00:00