Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence.


:Virulence in Shigella spp., as well as in strains of enteroinvasive Escherichia coli, is regulated by growth temperature. Previously, virR had been identified as the gene controlling the temperature-regulated expression of Shigella virulence. Since Shigella spp. and E. coli are also known to share greater than 90% DNA sequence homology, we sought to determine if nonpathogenic E. coli K-12 C600 contains a gene homologous to the Shigella flexneri 2a gene virR. Through the use of transduction and molecular cloning of strain C600 chromosomal DNA we have shown that E. coli K-12 does indeed contain a gene functionally homologous to the virR of S. flexneri.


J Bacteriol


Journal of bacteriology


Hromockyj AE,Maurelli AT




Has Abstract


1989-05-01 00:00:00












  • Transcriptional organization of the phycocyanin subunit gene clusters of the cyanobacterium Anacystis nidulans UTEX 625.

    abstract::The phycocyanin subunit gene cluster is duplicated on the chromosome of the cyanobacterium Anacystis nidulans UTEX 625. The two gene clusters cpcB1A1 (left) and cpcB2A2 (right) are separated by about 2,500 base pairs, and in each cluster the beta-subunit gene is located upstream from the alpha-subunit gene. Filter hyb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kalla SR,Lind LK,Lidholm J,Gustafsson P

    更新日期:1988-07-01 00:00:00

  • Streptokinase mutations relieving Escherichia coli K-12 (prlA4) of detriments caused by the wild-type skc gene.

    abstract::A novel phenotype is described for Escherichia coli K-12 carrying the prlA4 allele determining a membrane component of the protein export mechanism. It is manifest as transformation deficiency for plasmids containing the cloned group C streptococcal streptokinase gene, skc. Streptokinase plasmid mutations relieving th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Müller J,Reinert H,Malke H

    更新日期:1989-04-01 00:00:00

  • IS1634, a novel insertion element creating long, variable-length direct repeats which is specific for Mycoplasma mycoides subsp. mycoides small-colony type.

    abstract::A new insertion sequence, IS1634, has been identified in Mycoplasma mycoides subsp. mycoides small-colony type (SC). IS1634 shows structural and functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of insertion sequences. IS1634 has a size of 1,872 bp, including ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vilei EM,Nicolet J,Frey J

    更新日期:1999-02-01 00:00:00

  • Novel genomic rearrangement that affects expression of the Streptococcus pyogenes streptolysin O (slo) gene.

    abstract::A RecA-independent chromosomal rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes which affects slo expression was identified. PCR analysis was used to demonstrate that this kind of rearrangement was found in several strains of different lineages. Chromosomal loci involved ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Savic DJ,Ferretti JJ

    更新日期:2003-03-01 00:00:00

  • Caulobacter crescentus pili: structure and stage-specific expression.

    abstract::Pili are functionally expressed during the predivisional and swarmer stages of the Caulobacter crescentus differentiation cycle. They appear on the developing swarmer pole and at the same cellular location as flagella and the phiCbK receptor sites. Pili disappear when the swarmer cell differentiates into a stalked cel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lagenaur C,Agabian N

    更新日期:1977-07-01 00:00:00

  • Requirement for autoinducer in transcriptional negative autoregulation of the Vibrio fischeri luxR gene in Escherichia coli.

    abstract::The effect of a mutation in luxI (autoinducer synthetase gene) on transcription of luxR in the cloned Vibrio fischeri lux system (luxR, luxICDABE) was examined in Escherichia coli. For the luxI mutant, transcription from the luxR promoter (monitored with beta-galactosidase levels from a luxR::lacZ fusion, with LuxR su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dunlap PV,Ray JM

    更新日期:1989-06-01 00:00:00

  • Imprecise excision of plasmid pE194 from the chromosomes of Bacillus subtilis pE194 insertion strains.

    abstract::Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Conrad B,Bashkirov VI,Hofemeister J

    更新日期:1992-11-01 00:00:00

  • Evidence that asgB encodes a DNA-binding protein essential for growth and development of Myxococcus xanthus.

    abstract::The asg mutants of Myxococcus xanthus are defective in production of extracellular A-signal, which serves as a cell density signal for fruiting-body development. The DNA sequence of asgB, one of the three asg genes, was determined. The deduced amino acid sequence of AsgB contains a DNA-binding helix-turn-helix motif n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Plamann L,Davis JM,Cantwell B,Mayor J

    更新日期:1994-04-01 00:00:00


    abstract::Martin, Alice J. (Nassau County Department of Health, Hempstead, N.Y.), and Margaret O'Brien. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bacteriol. 89:570-573. 1965.-The application of the fluorescent-antibody (FA) technique to cultures of f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: MARTIN AJ,O'BRIEN M

    更新日期:1965-03-01 00:00:00

  • HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum.

    abstract::Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Escamilla-Hernandez R,O'Brian MR

    更新日期:2012-06-01 00:00:00

  • Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.

    abstract::A procedure is presented for the rapid screening of bacterial colonies to detect mutants unable to produce 14CO2 from a labeled precursor. The method is especially useful for mass screening for mutants that cannot be easily detected by their phenotypic characteristics. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tabor H,Tabor CW,Hafner EW

    更新日期:1976-10-01 00:00:00

  • Novel monofunctional histidinol-phosphate phosphatase of the DDDD superfamily of phosphohydrolases.

    abstract::The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lee HS,Cho Y,Lee JH,Kang SG

    更新日期:2008-04-01 00:00:00

  • DRS is far less divergent than streptococcal inhibitor of complement of group A streptococcus.

    abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sagar V,Kumar R,Ganguly NK,Menon T,Chakraborti A

    更新日期:2007-04-01 00:00:00

  • Temperature-sensitive lesions in the Francisella novicida valA gene cloned into an Escherichia coli msbA lpxK mutant affecting deoxycholate resistance and lipopolysaccharide assembly at the restrictive temperature.

    abstract::The valAB locus of Francisella novicida has previously been found to be highly similar at the deduced amino acid level to msbA lpxK of Escherichia coli. Both ValA and MsbA are members of the superfamily of ABC transporters, and they appear to have similar functions. In this study we describe the isolation of a tempera...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McDonald MK,Cowley SC,Nano FE

    更新日期:1997-12-01 00:00:00

  • Identification of a plasmid-borne locus in Rhizobium etli KIM5s involved in lipopolysaccharide O-chain biosynthesis and nodulation of Phaseolus vulgaris.

    abstract::Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vinuesa P,Reuhs BL,Breton C,Werner D

    更新日期:1999-09-01 00:00:00

  • RSF1010 plasmid as a potentially useful vector in Pseudomonas species.

    abstract::RSF1010 plasmid DNA was introduced into Pseudomonas putida and P. aeruginosa cells and maintained stably, suggesting the potential usefulness of this plasmid as a vector in Pseudomonas species. The number of copies of RSF1010 was 43 per chromosome equivalent in P. putida cells. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nagahari K,Sakaguchi K

    更新日期:1978-03-01 00:00:00

  • Naturally occurring Staphylococcus epidermidis plasmid expressing constitutive macrolide-lincosamide-streptogramin B resistance contains a deleted attenuator.

    abstract::A naturally occurring constitutive macrolide-lincosamide-streptogramin B (MLS) resistance plasmid, pNE131, from Staphylococcus epidermidis was chosen to study the molecular basis of constitutive expression. Restriction and functional maps of pNE131 are presented along with the nucleotide sequence of ermM, the gene whi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lampson BC,Parisi JT

    更新日期:1986-05-01 00:00:00

  • Effect of carbon source and the role of cyclic adenosine 3',5'-monophosphate on the Caulobacter cell cycle.

    abstract::The expression of cell cycle events in Caulobacter crescentus CB13 has been shown to be associated with regulation of carbohydrate utilization. Growth on lactose and galactose depends on induction of specific enzymes. Prior growth on glucose results in a delay in enzyme expression and cell cycle arrest at the nonmotil...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kurn N,Shapiro L,Agabian N

    更新日期:1977-09-01 00:00:00

  • Hexuronic acid dehydrogenase of Agrobacterium tumefaciens.

    abstract::Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chang YF,Feingold DS

    更新日期:1969-09-01 00:00:00

  • Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes.

    abstract::Using physical and genetic data, we have demonstrated that Rhizobium meliloti SU47 has a symbiotic megaplasmid, pRmeSU47b, in addition to the previously described nod-nif megaplasmid pRmeSU47a. This plasmid includes four loci involved in exopolysaccharide (exo) synthesis as well as two loci involved in thiamine biosyn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Finan TM,Kunkel B,De Vos GF,Signer ER

    更新日期:1986-07-01 00:00:00

  • Identification and Characterization of the Major Porin of Desulfovibrio vulgaris Hildenborough.

    abstract::Due in large part to their ability to facilitate the diffusion of a diverse range of solutes across the outer membrane (OM) of Gram-negative bacteria, the porins represent one of the most prominent and important bacterial membrane protein superfamilies. Notably, for the Gram-negative bacterium Desulfovibrio vulgaris H...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zeng L,Wooton E,Stahl DA,Walian PJ

    更新日期:2017-10-31 00:00:00

  • Role and regulation of Bacillus subtilis glutamate dehydrogenase genes.

    abstract::The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Belitsky BR,Sonenshein AL

    更新日期:1998-12-01 00:00:00

  • The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli.

    abstract::Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Thanedar S,Dineshkumar TK,Varshney U

    更新日期:2001-12-01 00:00:00

  • Relationships between fatty acid and polyketide synthases from Streptomyces coelicolor A3(2): characterization of the fatty acid synthase acyl carrier protein.

    abstract::We have characterized an acyl carrier protein (ACP) presumed to be involved in the synthesis of fatty acids in Streptomyces coelicolor A3(2). This is the third ACP to have been identified in S. coelicolor; the two previously characterized ACPs are involved in the synthesis of two aromatic polyketides: the blue-pigment...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Revill WP,Bibb MJ,Hopwood DA

    更新日期:1996-10-01 00:00:00

  • Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae.

    abstract::Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McCullough J,Herskowitz I

    更新日期:1979-04-01 00:00:00

  • Metabolism of dibutylphthalate and phthalate by Micrococcus sp. strain 12B.

    abstract::Micrococcus sp. strain 12B was isolated by enriching for growth with dibutylphthalate as the sole carbon and energy source. A pathway for the metabolism of dibutylphthalate and phthalate by micrococcus sp. strain 12B is proposed: dibutylphthalate leads to monobutylphthalate leads to phthalate leads to 3,4-dihydro-3,4-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Eaton RW,Ribbons DW

    更新日期:1982-07-01 00:00:00

  • Spore germination in strains of Dictyostelium discoideum and other members of the Dictyosteliaceae.

    abstract::Spores of all strains of Dictyostelium discoideum tested in this study germinated after a heat shock of 45 C for 30 min. Whereas the strains differed in their rates of germination, the rate for each strain was constant. A correlation existed between the rate of germination and the rate of vegetative growth when spores...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cotter DA,Raper KB

    更新日期:1968-11-01 00:00:00

  • The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite.

    abstract::The Bacillus subtilis skin element confers resistance to arsenate and arsenite. The ars operon in the skin element contains four genes in the order arsR, ORF2, arsB, and arsC. Three of these genes are homologous to the arsR, arsB, and arsC genes from the staphylococcal plasmid pI258, while no homologs of ORF2 have bee...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sato T,Kobayashi Y

    更新日期:1998-04-01 00:00:00

  • Induced sensitization of normal laboratory animals to Brucella abortus endotoxin.

    abstract::A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were prev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Freedman HH,Fox AE,Willis RS,Schwartz BS

    更新日期:1968-02-01 00:00:00

  • Ribosomes of the extremely thermophilic eubacterium Thermotoga maritima are uniquely insensitive to the miscoding-inducing action of aminoglycoside antibiotics.

    abstract::Poly(U)- and poly(UG)-programmed cell-free systems were developed from the extreme thermophilic, anaerobic eubacterium Thermotoga maritima, and their susceptibility to aminoglycoside and other antibiotics was assayed at a temperature (75 degrees C) close to the physiological optimum (80 degrees C) for cell growth and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Londei P,Altamura S,Huber R,Stetter KO,Cammarano P

    更新日期:1988-09-01 00:00:00