Abstract:
:The Saccharomyces cerevisiae HIS7 gene was cloned by its location immediately downstream of the previously isolated and characterized ARO4 gene. The two genes have the same orientation with a distance of only 416 bp between the two open reading frames. The yeast HIS7 gene represents the first isolated eukaryotic gene encoding the enzymatic activities which catalyze the fifth and sixth step in histidine biosynthesis. The open reading frame of the HIS7 gene has a length of 1,656 bp resulting in a gene product of 552 amino acids with a calculated molecular weight of 61,082. Two findings implicate a bifunctional nature of the HIS7 gene product. First, the N-terminal and C-terminal segments of the deduced HIS7 amino acid sequence show significant homology to prokaryotic monofunctional glutamine amidotransferases and cyclases, respectively, involved in histidine biosynthesis. Second, the yeast HIS7 gene is able to suppress His auxotrophy of corresponding Escherichia coli hisH and hisF mutants. HIS7 gene expression is regulated by the general control system of amino acid biosynthesis. GCN4-dependent and GCN4-independent (basal) transcription use different initiator elements in the HIS7 promoter.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kuenzler M,Balmelli T,Egli CM,Paravicini G,Braus GHdoi
10.1128/jb.175.17.5548-5558.1993subject
Has Abstractpub_date
1993-09-01 00:00:00pages
5548-58issue
17eissn
0021-9193issn
1098-5530journal_volume
175pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1967-01-01 00:00:00
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更新日期:1985-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1328-1333.1969
更新日期:1969-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1570-1577.1994
更新日期:1994-03-01 00:00:00
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更新日期:2011-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2006-09-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1973-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1973-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1977-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1971-09-01 00:00:00