Abstract:
:This paper describes the major transmutations of donor deoxyribonucleic acid (DNA) after uptake by competent Bacillus subtilis cells. Kinetic experiments confirm that after exposure to competent cells, donor DNA is converted to double-stranded fragments (DSF) which can be isolated as early as 30 s from the beginning of the reaction. At this time, DSF represent the only identifiable product of donor origin. After 1 to 2 min, DSF are converted to deoxyribonuclease-resistant forms, identified as single-stranded DNA fragments (SSF). SSF are intermediates in the transformation process leading to the formation of donor-recipient complex. This component makes its appearance between 2 to 4 min from the beginning of the transformation process. All the donor-recipient complexes found at the end of the reaction can be accounted for quantitatively by the DSF and the SSF found in the initial stages of transformation. A quantitative discussion of the transformation process is included.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Davidoff-Abelson R,Dubnau Ddoi
10.1128/JB.116.1.154-162.1973subject
Has Abstractpub_date
1973-10-01 00:00:00pages
154-62issue
1eissn
0021-9193issn
1098-5530journal_volume
116pub_type
杂志文章abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...
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doi:10.1128/jb.170.3.1399-1402.1988
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journal_title:Journal of bacteriology
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doi:10.1128/JB.127.1.367-379.1976
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pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
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更新日期:1979-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.122.3.826-831.1975
更新日期:1975-06-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.167.2.703-710.1986
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journal_title:Journal of bacteriology
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doi:10.1128/jb.166.2.689-692.1986
更新日期:1986-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2001-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7643-7649.1991
更新日期:1991-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1973-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2006-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2399-2402.1968
更新日期:1968-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.715-719.1980
更新日期:1980-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1972-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1978-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.15.3992-3996.1998
更新日期:1998-08-01 00:00:00
abstract::Among its penicillin-binding proteins (PBPs), Enterococcus faecium possesses a low-affinity PBP5, PBP5fm, which is the main target involved in beta-lactam resistance. A 7.7-kb EcoRI chromosomal fragment of E. faecium D63r containing the pbp5fm gene was cloned and sequenced. Two open reading frames (ORFs) were found. A...
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pub_type: 杂志文章
doi:10.1128/jb.178.16.4948-4957.1996
更新日期:1996-08-01 00:00:00