Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions.

Abstract:

:A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c reductase, cytochrome oxidase, and adenosine triphosphatase. In addition, two cytoplasmic mutants have been found whose primary defect is in cytochrome oxidase, and others have been found that show variable degrees of abnormalities in their mitochondrial translation products.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Tzagoloff A,Akai A,Needleman RB

doi

10.1128/JB.122.3.826-831.1975

subject

Has Abstract

pub_date

1975-06-01 00:00:00

pages

826-31

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

122

pub_type

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