Abstract:
:Using an oligonucleotide microarray, we searched for previously unrecognized transcription units in intergenic regions in the genome of Bacillus subtilis, with an emphasis on identifying small genes activated during spore formation. Nineteen transcription units were identified, 11 of which were shown to depend on one or more sporulation-regulatory proteins for their expression. A high proportion of the transcription units contained small, functional open reading frames (ORFs). One such newly identified ORF is a member of a family of six structurally similar genes that are transcribed under the control of sporulation transcription factor σ(E) or σ(K). A multiple mutant lacking all six genes was found to sporulate with slightly higher efficiency than the wild type, suggesting that under standard laboratory conditions the expression of these genes imposes a small cost on the production of heat-resistant spores. Finally, three of the transcription units specified small, noncoding RNAs; one of these was under the control of the sporulation transcription factor σ(E), and another was under the control of the motility sigma factor σ(D).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schmalisch M,Maiques E,Nikolov L,Camp AH,Chevreux B,Muffler A,Rodriguez S,Perkins J,Losick Rdoi
10.1128/JB.00534-10subject
Has Abstractpub_date
2010-10-01 00:00:00pages
5402-12issue
20eissn
0021-9193issn
1098-5530pii
JB.00534-10journal_volume
192pub_type
杂志文章abstract::The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6859-6864.1991
更新日期:1991-11-01 00:00:00
abstract::The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00122-06
更新日期:2006-06-01 00:00:00
abstract::Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00826-16
更新日期:2017-02-28 00:00:00
abstract::A 3-hydroxypropionate/4-hydroxybutyrate cycle operates in autotrophic CO(2) fixation in various Crenarchaea, as studied in some detail in Metallosphaera sedula. This cycle and the autotrophic 3-hydroxypropionate cycle in Chloroflexus aurantiacus have in common the conversion of acetyl-coenzyme A (CoA) and two bicarbon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00068-09
更新日期:2009-07-01 00:00:00
abstract::Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from hu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00222-09
更新日期:2009-06-01 00:00:00
abstract::Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.300-308.1971
更新日期:1971-10-01 00:00:00
abstract::pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4809-4812.1995
更新日期:1995-08-01 00:00:00
abstract::Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1628-1633.1990
更新日期:1990-03-01 00:00:00
abstract::The virulence of Pseudomonas aeruginosa and other surface pathogens involves the coordinate expression of a wide range of virulence determinants, including type IV pili. These surface filaments are important for the colonization of host epithelial tissues and mediate bacterial attachment to, and translocation across, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00157-06
更新日期:2006-12-01 00:00:00
abstract::As with other inducible enzymes, the induced synthesis of l-arabinose isomerase (l-arabinose ketol isomerase, EC 5.3.1.4) in Salmonella typhimurium is subject to catabolite repression. Of the three catabolite repressors tested, glucose produces maximum repression. Analogues of catabolite repressors like 2-deoxy-d-gluc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.1.107-112.1971
更新日期:1971-04-01 00:00:00
abstract::Aronson, John N. (Arizona State University, Tempe), and Gerald R. Wermus. Effects of m-tyrosine on growth and sporulation of Bacillus species. J. Bacteriol. 90:38-46. 1965.-The aromatic amino acid analogue, dl-beta-(3-hydroxyphenyl)alanine (m-tyrosine), reduced sporulation of a strain of Bacillus subtilis to less than...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.1.38-46.1965
更新日期:1965-07-01 00:00:00
abstract::Isolated sheaths of Leptothrix discophora SP-6 (ATCC 51168) were tested for susceptibility to degradation by a variety of chemical denaturants and lytic enzymes and found to be resistant to many reagents and enzyme treatments. However, disulfide bond-reducing agents such as dithiothreitol (DTT), beta-mercaptoethanol, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.7819-7827.1993
更新日期:1993-12-01 00:00:00
abstract::The vitamin requirements of the group Q streptococci (Streptococcus avium) and the established enterococcal species (S. faecalis and S. faecium) paralleled one another, although S. avium did not characteristically require riboflavine or pyridoxal. S. avium was further differentiated in that it required thymine for gro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.2.297-299.1967
更新日期:1967-08-01 00:00:00
abstract::The tar and tap loci of Escherichia coli encode methyl-accepting inner membrane proteins that mediate chemotactic responses to aspartate and maltose or to dipeptides. These genes lie adjacent to each other in the same orientation on the chromosome and have extensive sequence homology throughout the C-terminal portions...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.7.2938-2944.1987
更新日期:1987-07-01 00:00:00
abstract::We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.458-460.1985
更新日期:1985-01-01 00:00:00
abstract::A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.35-45.1996
更新日期:1996-01-01 00:00:00
abstract::Salmonella enterica encodes a type III secretion system within a pathogenicity island located at centisome 63 that is essential for virulence. All type III secretion systems require the function of a family of low-molecular-weight proteins that aid the secretion process by acting as partitioning factors and/or secreti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.8.2262-2268.2000
更新日期:2000-04-01 00:00:00
abstract::Filaments of a thermosensitive Bacillus megaterium mutant showed an altered macromolecular composition compared with salt-cured mutant cells and parental cells. Filaments contained more peptidoglycan, polyglucose, poly-beta-hydroxy-butyrate, and deoxyribonucleic acid per unit of protein. The ribonucleic acid-to-protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.454-456.1980
更新日期:1980-10-01 00:00:00
abstract::An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.5.2063-2069.1988
更新日期:1988-05-01 00:00:00
abstract::Sporulation-related repeat (SPOR) domains are small peptidoglycan (PG) binding domains found in thousands of bacterial proteins. The name "SPOR domain" stems from the fact that several early examples came from proteins involved in sporulation, but SPOR domain proteins are quite diverse and contribute to a variety of p...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00118-17
更新日期:2017-06-27 00:00:00
abstract::Aggregative amoebae of Dictyostelium discoideum, D. mucoroides, D. purpureum, and D. rosarium react chemotactically to cyclic AMP (cAMP). We measured the chemotactic activity of 14 cAMP analogs and found that these four species have a similar sensitivity to chemical modifications of cAMP; this suggests that the cAMP r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.99-105.1982
更新日期:1982-01-01 00:00:00
abstract::The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.525-530.1990
更新日期:1990-02-01 00:00:00
abstract::In contrast to the absolute Na(+) requirement for anaerobic growth of Aerobacter aerogenes on citrate as sole carbon source, aerobic growth of this microorganism did not require the presence of Na(+). However, Na(+) (optimal concentration, 10 mm) did increase the maximal amount of aerobic growth by 60%, even though it...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.2.389-394.1969
更新日期:1969-08-01 00:00:00
abstract::The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.14.4328-4337.1994
更新日期:1994-07-01 00:00:00
abstract::The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.553-562.1986
更新日期:1986-11-01 00:00:00
abstract::Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.3.1438-1440.1972
更新日期:1972-12-01 00:00:00
abstract::Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. We report here the isolation of a new class of K. pneumoniae mutants which exhibit altered pattern...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.597-606.1979
更新日期:1979-11-01 00:00:00
abstract::Smith, Rodney F. (University of Maryland, Baltimore), Donald E. Shay, and Norman J. Doorenbos. Antimicrobial action of nitrogen-containing steroids. J. Bacteriol. 85:1295-1299. 1963.-A new group of 16 synthetic nitrogen-containing steroids have been tested against a variety of microorganisms for antimicrobial properti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.6.1295-1299.1963
更新日期:1963-06-01 00:00:00
abstract::An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.7.1851-1858.2002
更新日期:2002-04-01 00:00:00
abstract::We show that hisT function is required for normal growth of Escherichia coli K-12, since a lack of hisT-mediated pseudouridine tRNA modification causes a uracil requirement that interferes with cell division. We also show that hisT transcription is positively growth rate regulated in exponentially growing bacteria and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.22.7395-7400.1991
更新日期:1991-11-01 00:00:00