Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells.

Abstract:

:Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and about 75% of the DNA is membrane associated. DNA, ribonucleic acid, and protein synthesis proceeds in R(+) minicells in contrast to R(-) minicell controls. The system offers a unique opportunity to study a relatively small replicative unit in a native cell environment and a simple means of isolating large quantities of episomal DNA.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Levy SB

doi

10.1128/JB.108.1.300-308.1971

subject

Has Abstract

pub_date

1971-10-01 00:00:00

pages

300-8

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

108

pub_type

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