Cloning and characterization of the gene cluster for palatinose metabolism from the phytopathogenic bacterium Erwinia rhapontici.

abstract：:The morphology of nucleoids and mesosomes of Bacillus subtilis in stationary-and lag-phase cultures was studied by making three-dimensional cell reconstructions in plastic of electron micrographs of serial sections. In cells from stationary cultures, the dormant nucleoids are frequently, but not always, spherical and ...

journal_title：Journal of bacteriology

authors： Van Iterson W,Michels PA,Vyth-Dreese F,Aten JA

更新日期：1975-03-01 00:00:00

abstract：:Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...

journal_title：Journal of bacteriology

authors： Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl B

更新日期：2010-11-01 00:00:00

abstract：:We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a m...

journal_title：Journal of bacteriology

authors： Beenken KE,Dunman PM,McAleese F,Macapagal D,Murphy E,Projan SJ,Blevins JS,Smeltzer MS

更新日期：2004-07-01 00:00:00

abstract：:Wall turnover was studied in Bacillus subtilis. The loss of radioactively labeled wall polymers was followed during exponential growth in batch and chemostat cultures. Turnover kinetics were identical under all growth conditions; pulse-labeled wall material was lost with first-order kinetics, but only after exponentia...

journal_title：Journal of bacteriology

authors： De Boer WR,Kruyssen FJ,Wouters JT

更新日期：1981-01-01 00:00:00

abstract：:Satellite bacteriophage P4 requires the products of the late genes of a helper such as P2 in order to grow lytically. The Escherichia coli rpoA109 mutation, which alters the alpha subunit of RNA polymerase, prevents transcription of the late genes of bacteriophage P2. Suppressor mutations that define the P2 ogr gene o...

journal_title：Journal of bacteriology

authors： Halling C,Sunshine MG,Lane KB,Six EW,Calendar R

更新日期：1990-07-01 00:00:00

abstract：:Methanohalophilus strain Z7302 was previously isolated from a hypersaline environment and grows over a range of NaCl concentrations from 1.7 to 4.4 M. We examined the relationships between cell growth rate, cell volume, and intracellular solute concentrations with increasing salinity. This extremely halophilic methano...

journal_title：Journal of bacteriology

authors： Lai MC,Gunsalus RP

更新日期：1992-11-01 00:00:00

abstract：:Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

journal_title：Journal of bacteriology

authors： Huyer M,Page WJ

更新日期：1989-07-01 00:00:00

abstract：:Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...

journal_title：Journal of bacteriology

authors： Crowe BA,Owen P

更新日期：1983-03-01 00:00:00

abstract：:Shewanella putrefaciens strain 200 respires a wide range of compounds as terminal electron acceptor. The respiratory versatility of Shewanella is attributed in part to a set of c-type cytochromes with widely varying midpoint redox potentials (E'(0)). A point mutant of S. putrefaciens, originally designated Urr14 and h...

journal_title：Journal of bacteriology

authors： Dale JR,Wade R Jr,Dichristina TJ

更新日期：2007-02-01 00:00:00

abstract：:The conjugation system of the IncP alpha plasmid RK2/RP4 is encoded by transfer regions designated Tra1, Tra2, and Tra3. The Tra1 core region, cloned on plasmid pDG4 delta 22, consists of the origin of transfer (oriT) and 2.6 kilobases of flanking DNA providing IncP alpha plasmid-specific functions that allow pDG4 del...

journal_title：Journal of bacteriology

authors： Guiney DG,Deiss C,Simnad V,Yee L,Pansegrau W,Lanka E

更新日期：1989-07-01 00:00:00

abstract：:The genome sequences of several pseudomonads have revealed a gene cluster containing genes for a two-component heavy metal histidine sensor kinase and response regulator upstream of cinA and cinQ, which we show herein to encode a copper-containing azurin-like protein and a pre-Q(0) reductase, respectively. In the pres...

journal_title：Journal of bacteriology

authors： Quaranta D,McCarty R,Bandarian V,Rensing C

更新日期：2007-07-01 00:00:00

abstract：:Spx is a global regulator that is widespread among the low-G+C-content gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under nonstress conditions, Spx is rapidly degraded by the ClpXP protease...

journal_title：Journal of bacteriology

authors： Engman J,Rogstam A,Frees D,Ingmer H,von Wachenfeldt C

更新日期：2012-03-01 00:00:00

abstract：:Salmonella enterica encodes a type III secretion system within a pathogenicity island located at centisome 63 that is essential for virulence. All type III secretion systems require the function of a family of low-molecular-weight proteins that aid the secretion process by acting as partitioning factors and/or secreti...

journal_title：Journal of bacteriology

authors： Tucker SC,Galán JE

更新日期：2000-04-01 00:00:00

abstract：:A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicate...

journal_title：Journal of bacteriology

authors： Sherman LA,van de Putte P

更新日期：1982-04-01 00:00:00

abstract：UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...

journal_title：Journal of bacteriology

authors： Kitanovic S,Ames P,Parkinson JS

更新日期：2015-08-01 00:00:00

abstract：:Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...

journal_title：Journal of bacteriology

authors： Lei MG,Cue D,Roux CM,Dunman PM,Lee CY

更新日期：2011-10-01 00:00:00

abstract：:Peptidoglycan was prepared from purified Bacillus subtilis spores of wild-type and several mutant strains. Digestion with muramidase resulted in cleavage of the glycosidic bonds adjacent to muramic acid replaced by peptide or alanine side chains but not the bonds adjacent to muramic lactam. Reduction of the resulting ...

journal_title：Journal of bacteriology

authors： Popham DL,Helin J,Costello CE,Setlow P

更新日期：1996-11-01 00:00:00

abstract：:A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...

journal_title：Journal of bacteriology

authors： Warner JR,Morgan SA,Shulman RW

更新日期：1976-03-01 00:00:00

abstract：:A RecA-independent chromosomal rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes which affects slo expression was identified. PCR analysis was used to demonstrate that this kind of rearrangement was found in several strains of different lineages. Chromosomal loci involved ...

journal_title：Journal of bacteriology

authors： Savic DJ,Ferretti JJ

更新日期：2003-03-01 00:00:00

abstract：:A gene (aad) coding for an aldehyde/alcohol dehydrogenase (AAD) was identified immediately upstream of the previously cloned ctfA (J. W. Cary, D. J. Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes...

journal_title：Journal of bacteriology

authors： Nair RV,Bennett GN,Papoutsakis ET

更新日期：1994-02-01 00:00:00

abstract：:The Shiga toxin operon (stx) is composed of two genes for the A and B subunits, which are transcribed from a promoter 5' to the stxA gene. The 1A:5B subunit stoichiometry of the holotoxin suggests that the stxA and stxB genes are differentially regulated. In a previous study, we demonstrated the existence of a second ...

journal_title：Journal of bacteriology

authors： Habib NF,Jackson MP

更新日期：1993-02-01 00:00:00

abstract：:Mature Fap1, a 200-kDa fimbria-associated adhesin, is required for fimbrial biogenesis and biofilm formation in Streptococcus parasanguis. Fap1-like proteins are found in the genomes of many streptococcal and staphylococcal species. Fap1 is a serine-rich glycoprotein modified by O-linked glycan moieties. In this study...

journal_title：Journal of bacteriology

authors： Wu H,Bu S,Newell P,Chen Q,Fives-Taylor P

更新日期：2007-02-01 00:00:00

abstract：:Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenin...

journal_title：Journal of bacteriology

authors： Mudd JB,Lee IM,Liu HY,Calavan EC

更新日期：1979-02-01 00:00:00

abstract：:Comamonas testosteroni T-2 uses a standard, if seldom examined, attack on an aromatic compound and oxygenates the side chain of p-toluenesulfonate (TS) (or p-toluenecarboxylate) to p-sulfobenzoate (or terephthalate) prior to complete oxidation. The expression of the first three catabolic enzymes in the pathway, the TS...

journal_title：Journal of bacteriology

authors： Junker F,Kiewitz R,Cook AM

更新日期：1997-02-01 00:00:00

abstract：:Rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. These cations were actively accumulated by the cells by a process following Michaelis-Menten saturation kinetics. The monovalent cation transport system had Km's of 0.2 mM K+, 0.5 mM Rb+, and 2.6 mM Cs+. The rates...

journal_title：Journal of bacteriology

authors： Jasper P

更新日期：1978-03-01 00:00:00

abstract：:The lemA gene of Pseudomonas syringae pv. syringae encodes the sensor kinase of a bacterial two-component signal transduction system. Phenotypes that are lemA dependent in P. syringae include lesion formation on bean and production of extracellular protease and the antibiotic syringomycin. Recently, the gacA gene has ...

journal_title：Journal of bacteriology

authors： Kitten T,Willis DK

更新日期：1996-03-01 00:00:00

abstract：:The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis conse...

journal_title：Journal of bacteriology

authors： Gunasekaran P,Karunakaran T,Cami B,Mukundan AG,Preziosi L,Baratti J

更新日期：1990-12-01 00:00:00

abstract：:Chemotaxis to the aromatic acid 4-hydroxybenzoate (4-HBA) by Pseudomonas putida is mediated by PcaK, a membrane-bound protein that also functions as a 4-HBA transporter. PcaK belongs to the major facilitator superfamily (MFS) of transport proteins, none of which have so far been implicated in chemotaxis. Work with two...

journal_title：Journal of bacteriology

authors： Ditty JL,Harwood CS

更新日期：1999-08-01 00:00:00

abstract：:The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...

journal_title：Journal of bacteriology

authors： Desiraju V,Shanabruch WG,Lu AL

更新日期：1993-01-01 00:00:00

abstract：:Spontaneous mutants that were resistant to zinc were isolated from Alcaligenes eutrophus CH34 containing either the native plasmid pMOL28 or a derivative derepressed for its self-transfer, pMOL50. With the cured plasmid-free derivative of CH34, strain AE104, such mutants were not detected. The mutations, which were sh...

journal_title：Journal of bacteriology

authors： Collard JM,Provoost A,Taghavi S,Mergeay M

更新日期：1993-02-01 00:00:00