A new type of Alcaligenes eutrophus CH34 zinc resistance generated by mutations affecting regulation of the cnr cobalt-nickel resistance system.

abstract：:Leucine and isoleucine-valine loci have been mapped in Rhizobium japonicum. Transformation analysis suggests a common pathway for isoleucine-valine biosynthesis. Three-point reciprocal crosses indicated that all the leucine loci are not genetically linked. ...

journal_title：Journal of bacteriology

authors： Doctor F,Modi VV

更新日期：1976-05-01 00:00:00

abstract：:Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...

journal_title：Journal of bacteriology

authors： Kuhla J,Oelze J

更新日期：1988-11-01 00:00:00

abstract：:During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular c...

journal_title：Journal of bacteriology

authors： Shiau SP,Schneider BL,Gu W,Reitzer LJ

更新日期：1992-01-01 00:00:00

abstract：:Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

journal_title：Journal of bacteriology

authors： Garcia DL,Dillard JP

更新日期：2008-06-01 00:00:00

abstract：:The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...

journal_title：Journal of bacteriology

authors： Khosla C,McDaniel R,Ebert-Khosla S,Torres R,Sherman DH,Bibb MJ,Hopwood DA

更新日期：1993-04-01 00:00:00

abstract：:Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster...

journal_title：Journal of bacteriology

authors： Claus H,Friedrich A,Frosch M,Vogel U

更新日期：2000-03-01 00:00:00

abstract：:The methanol-insoluble, heat-stable enterotoxin of Escherichia coli synthesized by clinical strains or strains that harbor the cloned gene was shown to be an extracellular polypeptide. The toxin (STB) was first detected as an 8,100-Mr precursor (pre-STB) that was converted to a transiently cell-associated 5,200-Mr for...

journal_title：Journal of bacteriology

authors： Kupersztoch YM,Tachias K,Moomaw CR,Dreyfus LA,Urban R,Slaughter C,Whipp S

更新日期：1990-05-01 00:00:00

abstract：:A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of protei...

journal_title：Journal of bacteriology

authors： Wolf DH,Fink GR

更新日期：1975-09-01 00:00:00

abstract：:The hyperthermophilic eubacterium Thermotoga maritima possesses an operon encoding an Hsp70 molecular chaperone protein and a protein with meaningful homology to the small heat shock protein family of chaperones. This represents the first demonstrated co-operon organization for these two important classes of molecular...

journal_title：Journal of bacteriology

authors： Michelini ET,Flynn GC

更新日期：1999-07-01 00:00:00

abstract：:The Escherichia coli plasmids pBR322 and pBR327 can be taken up by Haemophilus influenzae but do not replicate in this organism; however, integration of pBR into the H. influenzae chromosome was achieved by ligation to a fragment of the Haemophilus phage S2 that carried a phage attachment site (attP). Once these seque...

journal_title：Journal of bacteriology

authors： Kauc L,Goodgal SH

更新日期：1989-04-01 00:00:00

abstract：:A Bacillus subtilis ribose transport operon (rbs) was shown to be subject to AbrB-mediated control through direct AbrB-DNA binding interactions in the vicinity of the promoter. Overproduction of AbrB was shown to relieve catabolite repression of rbs during growth in the presence of poorer carbon sources such as arabin...

journal_title：Journal of bacteriology

authors： Strauch MA

更新日期：1995-12-01 00:00:00

abstract：:The organization and transcriptional control of chromosomal cat genes (required for dissimilation of catechol by the beta-ketoadipate pathway) in the Pseudomonas putida biotype strain (ATCC 12633) are reported. Nucleotide sequence reveals that catR is separated by 135 bp from the divergently transcribed catBC,A; catC ...

journal_title：Journal of bacteriology

authors： Houghton JE,Brown TM,Appel AJ,Hughes EJ,Ornston LN

更新日期：1995-01-01 00:00:00

abstract：:Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patter...

journal_title：Journal of bacteriology

authors： Dale JW,Al-Ghusein H,Al-Hashmi S,Butcher P,Dickens AL,Drobniewski F,Forbes KJ,Gillespie SH,Lamprecht D,McHugh TD,Pitman R,Rastogi N,Smith AT,Sola C,Yesilkaya H

更新日期：2003-04-01 00:00:00

abstract：:Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...

journal_title：Journal of bacteriology

authors： Leu YL,Wang PH,Shiao MS,Ismail W,Chiang YR

更新日期：2011-09-01 00:00:00

abstract：:Binding of Saccharomyces cerevisiae alpha-agglutinin to target a cells was assayed by agglutination inhibition and 125I-alpha-agglutinin binding. The assays showed characteristics of equilibrium binding, namely saturability, competability, and the establishment of a kinetic endpoint in the presence of free alpha-agglu...

journal_title：Journal of bacteriology

authors： Lipke PN,Terrance K,Wu YS

更新日期：1987-02-01 00:00:00

abstract：:Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...

journal_title：Journal of bacteriology

authors： MacGregor CH,Schnaitman CA

更新日期：1971-10-01 00:00:00

abstract：:Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and...

journal_title：Journal of bacteriology

authors： Kline BC,Schoenhard DE

更新日期：1969-10-01 00:00:00

abstract：:Two different 30-nucleotide regions of the cryIAc insecticidal protoxin gene from Bacillus thuringiensis were randomly mutagenized. One region was within one of seven amphipathic helices believed to be important for the formation of ion channels. There was no loss of toxicity for three test insects by any of 27 mutant...

journal_title：Journal of bacteriology

authors： Aronson AI,Wu D,Zhang C

更新日期：1995-07-01 00:00:00

abstract：:Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...

journal_title：Journal of bacteriology

authors： Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl B

更新日期：2010-11-01 00:00:00

abstract：:We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...

journal_title：Journal of bacteriology

authors： London RE,Allen DL,Gabel SA,DeRose EF

更新日期：1999-06-01 00:00:00

abstract：:Cell walls (LOG walls) were isolated from cultures of Streptococcus faecalis ATCC 9790 in the exponential phase of growth. These walls were either allowed to undergo autolytic dissolution (in the presence or absence of trypsin) or wall autolysis was inactivated with sodium dodecylsulfate (SDS walls). Inactivated walls...

journal_title：Journal of bacteriology

authors： Shockman GD,Martin JT

更新日期：1968-11-01 00:00:00

abstract：:The effect of sublethal, midlethal, and lethal doses of total-body X irradiation on the fecal microbial population of the rat was studied. No changes were observed in animals receiving sublethal doses of X ray. Midlethal and lethal doses produced an increase in the numbers of fecal coliforms, staphylococci, streptococ...

journal_title：Journal of bacteriology

authors： Klainer AS,Gorbach S,Weinstein L

更新日期：1967-08-01 00:00:00

abstract：:The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resi...

journal_title：Journal of bacteriology

authors： Kingsbury DT

更新日期：1967-09-01 00:00:00

abstract：:Evolution by natural selection occurs in cultures of Escherichia coli maintained under carbon starvation stress. Mutants of increased fitness express a growth advantage in stationary phase (GASP) phenotype, enabling them to grow and displace the parent as the majority population. The first GASP mutation was identified...

journal_title：Journal of bacteriology

authors： Zinser ER,Kolter R

更新日期：2000-08-01 00:00:00

abstract：:A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...

journal_title：Journal of bacteriology

authors： Wirth R,An FY,Clewell DB

更新日期：1986-03-01 00:00:00

abstract：:Modification of gonococcal deoxyribonucleic acid (DNA) was investigated, and the relationship with endonuclease production was explored. Both chromosomal and plasmid DNA from different gonococcal strains, irrespective of their plasmid content, was poorly cleaved by the restriction endonucleases HaeII, HaeIII, SacII, a...

journal_title：Journal of bacteriology

authors： Norlander L,Davies JK,Hagblom P,Normark S

更新日期：1981-02-01 00:00:00

abstract：:L-Glutamate decarboxylase (GAD) activity of Clostridium perfringens (ATCC 8009) cells grown in various culture conditions was investigated. Remarkable variations of GAD level occur during the growth cycle in thioglycollate broth. These changes are affected by the pH of the culture medium. Addition of alkali to the cul...

journal_title：Journal of bacteriology

authors： Cozzani I,Barsacchi R,Dibenedetto G,Saracchi L,Falcone G

更新日期：1975-09-01 00:00:00

abstract：:Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...

journal_title：Journal of bacteriology

authors： de Vries N,Duinsbergen D,Kuipers EJ,Pot RG,Wiesenekker P,Penn CW,van Vliet AH,Vandenbroucke-Grauls CM,Kusters JG

更新日期：2002-12-01 00:00:00

abstract：:After coliphage P1-mediated transfer of Tn5-containing plasmid DNA from Escherichia coli to Myxococcus xanthus, transductants were identified which contained plasmid sequences integrated at many sites on the bacterial chromosome. The unaltered plasmid DNA sequences in these transductants were apparently flanked by int...

journal_title：Journal of bacteriology

authors： Downard JS

更新日期：1988-10-01 00:00:00

abstract：:Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution w...

journal_title：Journal of bacteriology

authors： Morandi A,Zhaxybayeva O,Gogarten JP,Graf J

更新日期：2005-09-01 00:00:00