Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene.

abstract：:The nucleotide sequence of a 2.0-kilobase DNA segment containing the Clostridium acetobutylicum glnA gene was determined. The upstream region of the glnA gene contained two putative extended promoter consensus sequences (p1 and p2), characteristic of gram-positive bacteria. A third putative extended gram-positive prom...

journal_title：Journal of bacteriology

authors： Janssen PJ,Jones WA,Jones DT,Woods DR

更新日期：1988-01-01 00:00:00

abstract：:Isolated flagellar filaments of Sulfolobus shibatae were 15 nm in diameter, and they were composed of two major flagellins which have M(r)s of 31,000 and 33,000 and which stained positively for glycoprotein. The flagellar filaments of Thermoplasma volcanium were 12 nm in diameter and were composed of one major flagell...

journal_title：Journal of bacteriology

authors： Faguy DM,Bayley DP,Kostyukova AS,Thomas NA,Jarrell KF

更新日期：1996-02-01 00:00:00

abstract：:The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

journal_title：Journal of bacteriology

authors： Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

更新日期：1990-02-01 00:00:00

abstract：:The bfe locus codes for the cell surface receptor for vitamin B12, the E colicins, and bacteriophage BF23 in the Escherichia coli outer membrane. When the bfe+ allele, which is closely linked to the argH locus, was introduced into an argH bfe recipient by conjugation, arg+ recombinant cells rapidly and simultaneously ...

journal_title：Journal of bacteriology

authors： Bassford PJ Jr,Kadner RJ,Schnaitman CA

更新日期：1977-01-01 00:00:00

abstract：:We have constructed a suicide vector, pU1800, containing the transposable element TnphoA (Tn5 IS50L::phoA), for the purpose of producing protein fusions in vivo between the Escherichia coli alkaline phosphatase (APase) and proteins of the facultative photoheterotroph, Rhodobacter sphaeroides. We introduced TnphoA into...

journal_title：Journal of bacteriology

authors： Moore MD,Kaplan S

更新日期：1989-08-01 00:00:00

abstract：:Transposon mutagenesis was used to isolate mutants of Aeromonas hydrophila which were deficient in the production of extracellular proteins. The culture supernatants of two of the mutants were essentially devoid of the proteins normally secreted by the parent strain, despite their continued synthesis. Western immunobl...

journal_title：Journal of bacteriology

authors： Bo JN,Howard SP

更新日期：1991-02-01 00:00:00

abstract：:The function of SufI, a well-studied substrate of the TatABC translocase in Escherichia coli, is not known. It was earlier implicated in cell division, based on the finding that multiple copies of sufI suppressed the phenotypes of cells with mutations in ftsI (ftsI23), which encodes a divisomal transpeptidase. Recentl...

journal_title：Journal of bacteriology

authors： Samaluru H,SaiSree L,Reddy M

更新日期：2007-11-01 00:00:00

abstract：:The genetic context, phylogeny, and biochemistry of a gene flanking the H(2)-forming methylene-H(4)-methanopterin dehydrogenase gene (hmdA), here designated hmdB, indicate that it is a new member of the radical S-adenosylmethionine enzyme superfamily. In contrast to the characteristic CX(3)CX(2)C or CX(2)CX(4)C motif ...

journal_title：Journal of bacteriology

authors： McGlynn SE,Boyd ES,Shepard EM,Lange RK,Gerlach R,Broderick JB,Peters JW

更新日期：2010-01-01 00:00:00

abstract：:Rhizopines are selective growth substrates synthesized in nodules only by strains of rhizobia capable of their catabolism. We report the isolation and study of genes for the synthesis and catabolism of a new rhizopine, scyllo-inosamine (sIa), from alfalfa nodules induced by Rhizobium meliloti Rm220-3. This compound is...

journal_title：Journal of bacteriology

authors： Saint CP,Wexler M,Murphy PJ,Tempé J,Tate ME,Murphy PJ

更新日期：1993-08-01 00:00:00

abstract：:Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. T...

journal_title：Journal of bacteriology

authors： Tam JE,Pattee PA

更新日期：1986-11-01 00:00:00

abstract：:Campylobacter jejuni, a microaerophilic bacterium, is the most frequent cause of human bacterial gastroenteritis. C. jejuni is exposed to harmful reactive oxygen species (ROS) produced during its own normal metabolic processes and during infection from the host immune system and from host intestinal microbiota. These ...

journal_title：Journal of bacteriology

authors： Flint A,Sun YQ,Stintzi A

更新日期：2012-01-01 00:00:00

abstract：:The role of the carboxy terminus of the Escherichia coli cell division protein FtsA in bacterial division has been studied by making a series of short sequential deletions spanning from residue 394 to 420. Deletions as short as 5 residues destroy the biological function of the protein. Residue W415 is essential for th...

journal_title：Journal of bacteriology

authors： Yim L,Vandenbussche G,Mingorance J,Rueda S,Casanova M,Ruysschaert JM,Vicente M

更新日期：2000-11-01 00:00:00

abstract：:The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an omp...

journal_title：Journal of bacteriology

authors： Huang L,Tsui P,Freundlich M

更新日期：1992-02-01 00:00:00

abstract：:In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization to develop a techniq...

journal_title：Journal of bacteriology

authors： Fayet O,Prere MF

更新日期：1987-12-01 00:00:00

abstract：:A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

journal_title：Journal of bacteriology

authors： Calsou P,Salles B

更新日期：1985-06-01 00:00:00

abstract：:The inner membrane TET (TetA) protein, which is involved in Tn10-mediated microbial tetracycline resistance, consists of two domains, alpha and beta, both of which are needed for tetracycline resistance and efflux (M.S. Curiale, L.M. McMurry, and S.B. Levy, J. Bacteriol. 157:211-217, 1984). Since tetracycline-sensitiv...

journal_title：Journal of bacteriology

authors： Hickman RK,Levy SB

更新日期：1988-04-01 00:00:00

abstract：:Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

journal_title：Journal of bacteriology

authors： Friedberg I,Avigad G

更新日期：1968-08-01 00:00:00

abstract：:The role of the DNA primase of IncP plasmids was examined with a derivative of RP4 containing Tn7 in the primase gene (pri). The mutant was defective in mediating bacterial conjugation, with the deficiency varying according to the bacterial strains used as donors and recipients. Complementation tests involving recombi...

journal_title：Journal of bacteriology

authors： Merryweather A,Barth PT,Wilkins BM

更新日期：1986-07-01 00:00:00

abstract：:The virulence factors of the cariogenic bacterium Streptococcus sobrinus have been difficult to assess because of a lack of tools for the genetic manipulation of this organism. The construction of an Escherichia coli-Streptococcus shuttle vector, pDL289, that can be mobilized into S. sobrinus by the conjugative plasmi...

journal_title：Journal of bacteriology

authors： Buckley ND,Lee LN,LeBlanc DJ

更新日期：1995-09-01 00:00:00

abstract：:The presence of the membrane lipid phosphatidylcholine (PC) in the bacterial membrane is critically important for many host-microbe interactions. The phospholipid N-methyltransferase PmtA from the plant pathogen Agrobacterium tumefaciens catalyzes the formation of PC by a three-step methylation of phosphatidylethanola...

journal_title：Journal of bacteriology

authors： Aktas M,Gleichenhagen J,Stoll R,Narberhaus F

更新日期：2011-07-01 00:00:00

abstract：:Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...

journal_title：Journal of bacteriology

authors： Alonso-Vega P,Normand P,Bacigalupe R,Pujic P,Lajus A,Vallenet D,Carro L,Coll P,Trujillo ME

更新日期：2012-08-01 00:00:00

abstract：:Picolinic acid (PA), a typical C-2-carboxylated pyridine derivative, is a metabolite of l-tryptophan and many other aromatic compounds in mammalian and microbial cells. Microorganisms can degrade and utilize PA for growth. However, the precise mechanism of PA metabolism remains unknown. Alcaligenes faecalis strain JQ1...

journal_title：Journal of bacteriology

authors： Qiu J,Zhang Y,Yao S,Ren H,Qian M,Hong Q,Lu Z,He J

更新日期：2019-03-13 00:00:00

abstract：:Protein and mRNA levels of heat-labile enterotoxin (LT) of Escherichia coli are highest at 37 degrees C, and they decrease gradually as temperature is decreased. This temperature effect is eliminated in an Hns- mutant. Deletion of portions of DNA coding for the LT A subunit also results in an increase in LT expression...

journal_title：Journal of bacteriology

authors： Trachman JD,Maas WK

更新日期：1998-07-01 00:00:00

abstract：:A 4.2-kilobase cryptic Bacteroides plasmid, pB8-51, is found in several colonic Bacteroides species. To determine whether pB8-51 is mobilized by any of the known Bacteroides conjugal elements, we constructed an Escherichia coli-Bacteroides shuttle vector, pVAL-1, which contains pB8-51. We constructed Bacteroides unifo...

journal_title：Journal of bacteriology

authors： Valentine PJ,Shoemaker NB,Salyers AA

更新日期：1988-03-01 00:00:00

abstract：:Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highl...

journal_title：Journal of bacteriology

authors： Kan S,Fornelos N,Schuch R,Fischetti VA

更新日期：2013-10-01 00:00:00

abstract：:The bacteriophage P22-based challenge-phage system was used to study the binding of Xis and FIS to their sites in attP of bacteriophage lambda. Challenge phages were constructed that contained the X1, X2, and F sites within the P22 Pant promoter, which is required for expression of antirepressor. If Xis and FIS bind t...

journal_title：Journal of bacteriology

authors： Numrych TE,Gumport RI,Gardner JF

更新日期：1991-10-01 00:00:00

abstract：:Biofilms are structured multicellular communities of bacteria that form through a developmental process. In standing culture, undomesticated strains of Bacillus subtilis produce a floating biofilm, called a pellicle, with a distinct macroscopic architecture. Here we report on a comprehensive analysis of B. subtilis pe...

journal_title：Journal of bacteriology

authors： Kobayashi K

更新日期：2007-07-01 00:00:00

abstract：:The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...

journal_title：Journal of bacteriology

authors： Ge X,Lyu ZX,Liu Y,Wang R,Zhao XS,Fu X,Chang Z

更新日期：2014-02-01 00:00:00

abstract：:A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

journal_title：Journal of bacteriology

authors： Taylor BF,Campbell WL,Chinoy I

更新日期：1970-05-01 00:00:00

abstract：:Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...

journal_title：Journal of bacteriology

authors： Schrempf H,Bujard H,Hopwood DA,Goebel W

更新日期：1975-02-01 00:00:00