Method for localization of cloned DNA fragments on the Escherichia coli chromosome.

abstract：:Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a ...

journal_title：Journal of bacteriology

authors： Lindgren PB,Peet RC,Panopoulos NJ

更新日期：1986-11-01 00:00:00

abstract：:Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

journal_title：Journal of bacteriology

authors： Lucier TS,Brubaker RR

更新日期：1992-04-01 00:00:00

abstract：:Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly processive DNA helicase. The TraI relaxase activity is located within the N-terminal approximately 300 amino acids, while helicase motifs ...

journal_title：Journal of bacteriology

authors： Dostál L,Schildbach JF

更新日期：2010-07-01 00:00:00

abstract：:Fructose transport in Streptococcus mutans LG-1 is mediated by at least two distinct phosphoenolpyruvate fructose phosphotransferase systems. One system is constitutive and consists of membrane components enzyme II as well as enzyme I and heat-stable protein. The other system is inducible and requires, in addition to ...

journal_title：Journal of bacteriology

authors： Gauthier L,Mayrand D,Vadeboncoeur C

更新日期：1984-11-01 00:00:00

abstract：:Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...

journal_title：Journal of bacteriology

authors： Mickelson MN

更新日期：1967-07-01 00:00:00

abstract：:Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...

journal_title：Journal of bacteriology

authors： Younis R,Bingle LE,Rollauer S,Munera D,Busby SJ,Johnson S,Deane JE,Lea SM,Frankel G,Pallen MJ

更新日期：2010-11-01 00:00:00

abstract：:The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...

journal_title：Journal of bacteriology

authors： Usuda Y,Kawasaki H,Shimaoka M,Utagawa T

更新日期：1997-11-01 00:00:00

abstract：:Bacteria have evolved multiple strategies to sense and rapidly adapt to challenging and ever-changing environmental conditions. The ability to alter membrane lipid composition, a key component of the cellular envelope, is crucial for bacterial survival and adaptation in response to environmental stress. However, the p...

journal_title：Journal of bacteriology

authors： Rowlett VW,Mallampalli VKPS,Karlstaedt A,Dowhan W,Taegtmeyer H,Margolin W,Vitrac H

更新日期：2017-06-13 00:00:00

abstract：:The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...

journal_title：Journal of bacteriology

authors： Terrak M,Nguyen-Distèche M

更新日期：2006-04-01 00:00:00

abstract：:Peptidoglycan hydrolase, LytF (CwlE), was determined to be identical to YhdD (deduced cell wall binding protein) by zymography after insertional inactivation of the yhdD gene. YhdD exhibits high sequence similarity with CwlF (PapQ, LytE) and p60 of Listeria monocytogenes. The N-terminal region of YhdD has a signal seq...

journal_title：Journal of bacteriology

authors： Ohnishi R,Ishikawa S,Sekiguchi J

更新日期：1999-05-01 00:00:00

abstract：:Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

journal_title：Journal of bacteriology

authors： Chung YJ,Krueger C,Metzgar D,Saier MH Jr

更新日期：2001-02-01 00:00:00

abstract：:Goldberg, Herbert S. (University of Missouri, Columbia), Ella M. Barnes, and Anthony B. Charles. Unusual Bacteroides-like organism. J. Bacteriol. 87:737-742. 1964.-An organism is described which appears to be a new species of gram-negative, anaerobic, nonsporulating rod. It was isolated from poultry caeca at levels of...

journal_title：Journal of bacteriology

authors： GOLDBERG HS,BARNES EM,CHARLES AB

更新日期：1964-03-01 00:00:00

abstract：:Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...

journal_title：Journal of bacteriology

authors： Chaffin DO,Beres SB,Yim HH,Rubens CE

更新日期：2000-08-01 00:00:00

abstract：:sigma B of the gram-positive bacterium Bacillus subtilis is an alternative transcription factor activated by a variety of environmental stresses, including the stress imposed upon entry into the stationary growth phase. Previous reports have shown that this stationary-phase activation is enhanced when cells are grown ...

journal_title：Journal of bacteriology

authors： Wise AA,Price CW

更新日期：1995-01-01 00:00:00

abstract：:Spore cortex of conditional cortexless mutants of Bacillus sphaericus 9602 was not detectable by electron microscopy unless the medium was supplemented with meso-alpha,epsilon-diaminopimelic acid during sporulation. Other spore structures appeared normal. Spore shape was quite irregular in the absence of meso-alpha,ep...

journal_title：Journal of bacteriology

authors： Imae Y,Strominger MB,Strominger JL

更新日期：1976-09-01 00:00:00

abstract：:Erwinia rhapontici is able to convert sucrose into isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) by the activity of a sucrose isomerase. These sucrose isomers cannot be metabolized by plant cells and most other organisms and therefore are possi...

journal_title：Journal of bacteriology

authors： Börnke F,Hajirezaei M,Sonnewald U

更新日期：2001-04-01 00:00:00

abstract：:Biochemical events which occur during macroconidial germination have been studied in the dermatophyte Microsporum gypseum. The specific activity levels of various metabolic enzymes have been assayed during germination time periods. The accumulated levels of several of these enzymes, as a function of exogenous carbohyd...

journal_title：Journal of bacteriology

authors： Leighton TJ,Stock JJ,Kelln RA

更新日期：1970-08-01 00:00:00

abstract：:Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...

journal_title：Journal of bacteriology

authors： Helmstetter CE

更新日期：1974-10-01 00:00:00

abstract：:The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth r...

journal_title：Journal of bacteriology

authors： Kubitschek HE,Baldwin WW,Schroeter SJ,Graetzer R

更新日期：1984-04-01 00:00:00

abstract：:Proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. The mini-Tn5 transposon mutant mns2 was isolated as a tight nonswarming mutant that did not elongate or upregulate flagellar and hemolysin genes. Individual cell motility was ...

journal_title：Journal of bacteriology

authors： Hay NA,Tipper DJ,Gygi D,Hughes C

更新日期：1997-08-01 00:00:00

abstract：:RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate u...

journal_title：Journal of bacteriology

authors： Zhang S,Reeves A,Woodbury RL,Haldenwang WG

更新日期：2005-12-01 00:00:00

abstract：:The effects of the free bile acids (FBAs) cholic acid (CA), deoxycholic acid (DCA), and chenodeoxycholic acid on the bioenergetics and growth of lactobacilli and bifidobacteria were investigated. It was found that these FBAs reduced the internal pH levels of these bacteria with rapid and stepwise kinetics and, at cert...

journal_title：Journal of bacteriology

authors： Kurdi P,Kawanishi K,Mizutani K,Yokota A

更新日期：2006-03-01 00:00:00

abstract：:Streptococcus oralis, a commensal species of the human oral cavity, belongs to the Mitis group of streptococci, which includes one of the major human pathogens as well, S. pneumoniae. We report here the first complete genome sequence of this species. S. oralis Uo5, a high-level penicillin- and multiple-antibiotic-resi...

journal_title：Journal of bacteriology

authors： Reichmann P,Nuhn M,Denapaite D,Brückner R,Henrich B,Maurer P,Rieger M,Klages S,Reinhard R,Hakenbeck R

更新日期：2011-06-01 00:00:00

abstract：:We have constructed strains of Pseudomonas aeruginosa with mutations in the algC gene, previously shown to encode the enzyme phosphomannomutase. The algC mutants of a serotype O5 strain (PAO1) and a serotype O3 strain (PAC1R) did not express lipopolysaccharide (LPS) O side chains or the A-band (common antigen) polysac...

journal_title：Journal of bacteriology

authors： Coyne MJ Jr,Russell KS,Coyle CL,Goldberg JB

更新日期：1994-06-01 00:00:00

abstract：:The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...

journal_title：Journal of bacteriology

authors： Manna D,Wang X,Higgins NP

更新日期：2001-06-01 00:00:00

abstract：:A group of enzymes were prepared from the culture fluids of streptococci belonging to groups A, B, C, G, and L, and from a strain of Streptococcus sanguis. These streptococcal enzymes (designated St-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate BSM-St, a sialomuc...

journal_title：Journal of bacteriology

authors： Hayano S,Tanaka A

更新日期：1969-03-01 00:00:00

abstract：:Isolated membrane vesicles from the obligately acidophilic bacterium Bacillus acidocaldarius generated an electrochemical gradient of protons (delta mu- H+) upon energization with ascorbate-phenazine methosulfate at pH 6.0 or 3.0. At pH 6.0, there was little or no transmembrane pH gradient (delta pH), but a transmembr...

journal_title：Journal of bacteriology

authors： Guffanti AA,Mann M,Sherman TL,Krulwich TA

更新日期：1984-08-01 00:00:00

abstract：:The gene specifying a membrane-bound nucleoside diphosphate sugar hydrolase of Salmonella typhimurium was mapped near the metA locus by using intergeneric crosses between this bacterium and Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Minton N,Gunn J,Beacham IR

更新日期：1979-03-01 00:00:00

abstract：:Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...

journal_title：Journal of bacteriology

authors： van der Ploeg J,Willemsen M,van Hall G,Janssen DB

更新日期：1995-03-01 00:00:00

abstract：:Many microbes coexist within biofilms, or multispecies communities of cells encased in an extracellular matrix. However, little is known about the microbe-microbe interactions relevant for creating these structures. In this study, we explored a striking dual-species biofilm between Bacillus subtilis and Pantoea agglom...

journal_title：Journal of bacteriology

authors： Yannarell SM,Grandchamp GM,Chen SY,Daniels KE,Shank EA

更新日期：2019-08-22 00:00:00