Isolation of a novel protein involved in the transport of fructose by an inducible phosphoenolpyruvate fructose phosphotransferase system in Streptococcus mutans.

abstract：:The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...

journal_title：Journal of bacteriology

authors： Lafontaine ER,Cope LD,Aebi C,Latimer JL,McCracken GH Jr,Hansen EJ

更新日期：2000-03-01 00:00:00

abstract：:In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threo...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1974-01-01 00:00:00

abstract：:Rhizobium japonicum, capable of binding high-molecular-weight donor (32)P-labeled deoxyribonucleic acid (DNA) during late log phase in a competence medium, was transformed for streptomycin resistance with a frequency of transformation ranging between 0.02 and 0.08%. Eight to 10% of the homologous native (32)P-labeled ...

journal_title：Journal of bacteriology

authors： Raina JL,Modi VV

更新日期：1972-08-01 00:00:00

abstract：:Eklund, Curtis (The University of Texas, Austin) and Orville Wyss. Enzyme associated with bacteriophage infection. J. Bacteriol. 84:1209-1215. 1962.-A capsule-digesting enzyme was formed when azotobacter cells were infected with bacteriophage. The enzyme appeared in the medium when the phages lysed the cells. By disru...

journal_title：Journal of bacteriology

authors： Eklund C,Wyss O

更新日期：1962-12-01 00:00:00

abstract：:rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...

journal_title：Journal of bacteriology

authors： Schneider DA,Gourse RL

更新日期：2003-10-01 00:00:00

abstract：:PomA is thought to be a component of the ion channel in the sodium-driven polar-flagellar motor of Vibrio alginolyticus. We have found that some cysteine substitutions in the periplasmic region of PomA result in a slow-motility phenotype, in which swarming and swimming speeds are reduced even in the presence of high c...

journal_title：Journal of bacteriology

authors： Kojima S,Shoji T,Asai Y,Kawagishi I,Homma M

更新日期：2000-06-01 00:00:00

abstract：:sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...

journal_title：Journal of bacteriology

authors： Peters HK 3rd,Carlson HC,Haldenwang WG

更新日期：1992-07-01 00:00:00

abstract：:Mushin, Rose (University of Melbourne, Victoria, Australia) and Frances M. Ashburner. Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease. J. Bacteriol. 83:1260-1267. 1962.-The aerobic bacterial intestinal flora of mutton birds, healthy and affected with "limy" disease, wa...

journal_title：Journal of bacteriology

authors： MUSHIN R,ASHBURNER FM

更新日期：1962-06-01 00:00:00

abstract：:We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show tha...

journal_title：Journal of bacteriology

authors： Price KD,Losick R

更新日期：1999-02-01 00:00:00

abstract：:Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy an...

journal_title：Journal of bacteriology

authors： Mishra A,Das A,Cisar JO,Ton-That H

更新日期：2007-04-01 00:00:00

abstract：:We report that transformation of Streptomyces lividans with cloned DNA of the SLP1 genetic element results in integration of the element at the same chromosomal locus (attB) normally occupied by SLP1 in its original host, Streptomyces coelicolor, and in S. lividans that has received SLP1 by mating. We constructed SLP1...

journal_title：Journal of bacteriology

authors： Omer CA,Stein D,Cohen SN

更新日期：1988-05-01 00:00:00

abstract：:Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer th...

journal_title：Journal of bacteriology

authors： Wagner M,Roger AJ,Flax JL,Brusseau GA,Stahl DA

更新日期：1998-06-01 00:00:00

abstract：:Group I introns are inserted into genes of a wide variety of bacteriophages of gram-positive bacteria. However, among the phages of enteric and other gram-negative proteobacteria, introns have been encountered only in phage T4 and several of its close relatives. Here we report the insertion of a self-splicing group I ...

journal_title：Journal of bacteriology

authors： Bonocora RP,Shub DA

更新日期：2004-12-01 00:00:00

abstract：:The positions of the outer boundaries of the 5'- and 3'-conserved segment sequences of integrons found at several different locations have been determined. The position of the 5' end of the 5'-conserved segment is the same for six independently located integrons, In1 (R46), In2 (Tn21), In3 (R388), In4 (Tn1696), In5 (p...

journal_title：Journal of bacteriology

authors： Hall RM,Brown HJ,Brookes DE,Stokes HW

更新日期：1994-10-01 00:00:00

abstract：:Many bacteria can accumulate glycine betaine for osmoprotection and catabolize it as a growth substrate, but how they regulate these opposing roles is poorly understood. In Pseudomonas syringae B728a, expression of the betaine catabolism genes was reduced by an osmotic upshift to an intermediate stress level, consiste...

journal_title：Journal of bacteriology

authors： Li S,Yu X,Beattie GA

更新日期：2013-05-01 00:00:00

abstract：:Rhodocyclales are representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. Here we present the genome sequence of Methyloversatilis universalis FAM5(T), the first cultivable methylotrophic member of...

journal_title：Journal of bacteriology

authors： Kittichotirat W,Good NM,Hall R,Bringel F,Lajus A,Médigue C,Smalley NE,Beck D,Bumgarner R,Vuilleumier S,Kalyuzhnaya MG

更新日期：2011-09-01 00:00:00

abstract：:Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the tr...

journal_title：Journal of bacteriology

authors： Braunstein M,Griffin TJ IV,Kriakov JI,Friedman ST,Grindley ND,Jacobs WR Jr

更新日期：2000-05-01 00:00:00

abstract：:Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcripti...

journal_title：Journal of bacteriology

authors： Kim Y,Lew CM,Gralla JD

更新日期：2006-11-01 00:00:00

abstract：:Under static growth conditions, hyperpiliated, nontwitching pilT and pilU mutants of Pseudomonas aeruginosa formed dense biofilms, showing that adhesion, not twitching motility, is necessary for biofilm initiation. Under flow conditions, the pilT mutant formed mushroom-like structures larger than those of the wild typ...

journal_title：Journal of bacteriology

authors： Chiang P,Burrows LL

更新日期：2003-04-01 00:00:00

abstract：:The O-repeating unit of the Escherichia coli O7-specific lipopolysaccharide is made of galactose, mannose, rhamnose, 4-acetamido-4,6-dideoxyglucose, and N-acetyglucosamine. We have recently characterized the genes involved in the biosynthesis of the sugar precursor GDP-mannose occurring in the E. coli O7:K1 strain VW1...

journal_title：Journal of bacteriology

authors： Marolda CL,Valvano MA

更新日期：1995-10-01 00:00:00

abstract：:Membrane vesicles were characterized for their ability to specifically bind [14C]glucose 6-phosphate. Membranes prepared from a strain carrying a ColE1 uhp hybrid plasmid showed significantly enhanced glucose 6-phosphate binding. It is hypothesized that glucose 6-phosphate binding to these membranes is due to a uhpR-d...

journal_title：Journal of bacteriology

authors： Goldenbaum PE,Farmer KS

更新日期：1980-04-01 00:00:00

abstract：:The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indica...

journal_title：Journal of bacteriology

authors： Schlindwein C,Giordano G,Santini CL,Mandrand MA

更新日期：1990-10-01 00:00:00

abstract：:Previous investigations have demonstrated the existence of five Histoplasma capsulatum serotypes. Available specific fluorescent-antibody reagents stain only four of the five serotypes. Antibodies produced against the most complete H. capsulatum serotype were labeled with fluorescein isothiocyanate to develop a reagen...

journal_title：Journal of bacteriology

authors： Kaufman L,Blumer S

更新日期：1968-04-01 00:00:00

abstract：:Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated ou...

journal_title：Journal of bacteriology

authors： Bosch R,Moore ER,García-Valdés E,Pieper DH

更新日期：1999-04-01 00:00:00

abstract：:Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

journal_title：Journal of bacteriology

authors： Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

更新日期：1995-11-01 00:00:00

abstract：:When transport of polyamines in Escherichia coli was examined, putrescine excretion was observed under two different physiological conditions: (i) strictly correlated to growth and (ii) following a hyperosmotic shock. Spermidine was not excreted. Characterization of a deletion mutant showed that PotE is not involved i...

journal_title：Journal of bacteriology

authors： Schiller D,Kruse D,Kneifel H,Krämer R,Burkovski A

更新日期：2000-11-01 00:00:00

abstract：:Oxolinic acid reduced RNA synthesis rates whether chromosome supercoiling decreased, increased, or remained unchanged. Thus, inhibition of RNA synthesis by oxolinic acid appears to involve factors other than average DNA supercoiling level. Coumermycin A1 caused RNA synthesis rates to increase or decrease roughly in pa...

journal_title：Journal of bacteriology

authors： Manes SH,Pruss GJ,Drlica K

更新日期：1983-07-01 00:00:00

abstract：:Both alpha-tocopherolquinol and alpha-tocopherolquinone were found in 56 of 93 strains of microorganisms examined. Organisms that contained these compounds included the single example of a eucaryotic alga, a Euglena, and a cyanobacterium (blue-green alga), 22 of 32 genera of bacteria, and 9 genera of yeasts. In the ba...

journal_title：Journal of bacteriology

authors： Hughes PE,Tove SB

更新日期：1982-09-01 00:00:00

abstract：:The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...

journal_title：Journal of bacteriology

authors： Rodriguez RL,Davern CI

更新日期：1976-01-01 00:00:00

abstract：:In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...

journal_title：Journal of bacteriology

authors： Vimr ER,Troy FA

更新日期：1985-11-01 00:00:00