Abstract:
:Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a hypersensitive response on tobacco plants were identified. Six of these mutants no longer produced disease lesions on primary leaves of the susceptible bean cultivar Red Kidney and failed to elicit a hypersensitive response on the resistant bean cultivar Red Mexican and on the nonhost plants tomato, cowpea, and soybean. The two remaining mutants had reduced pathogenicity on Red Kidney bean and elicited variable hypersensitive responses on the other plants tested. Southern blot analysis indicated that each mutant carried a single independent Tn5 insertion in one of three EcoRI fragments of about 17, 7, and 5 kilobases. Marker exchange mutagenesis further supported the conclusion that the pleiotropic mutant phenotype was not associated with multiple Tn5 insertions. A genomic library of the wild-type strain was constructed in the cosmid vector pLAFR3. A recombinant plasmid, designated pPL6, that carried P. syringae pv. "phaseolicola" genomic sequences was identified by colony hybridization. This plasmid restored the wild-type phenotype to all but one mutant, suggesting that genes affected by the insertions were clustered. Structural analysis of pPL6 and the wild-type genome indicated that the 17- and 5-kilobase EcoRI fragments were contiguous in the strain NPS3121 genome.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lindgren PB,Peet RC,Panopoulos NJdoi
10.1128/jb.168.2.512-522.1986subject
Has Abstractpub_date
1986-11-01 00:00:00pages
512-22issue
2eissn
0021-9193issn
1098-5530journal_volume
168pub_type
杂志文章abstract::The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4637-4639.2000
更新日期:2000-08-01 00:00:00
abstract::The transduction efficiency of Tn551, a staphylococcal transposon coding for erythromycin resistance (ermB), was increased by a factor of about five in acceptor strains carrying the gene for methicillin resistance (mec), as compared with methicillin-sensitive strains. This enhancement was independent of the generalize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.533-535.1983
更新日期:1983-04-01 00:00:00
abstract::Satellite bacteriophage P4 requires the products of the late genes of a helper such as P2 in order to grow lytically. The Escherichia coli rpoA109 mutation, which alters the alpha subunit of RNA polymerase, prevents transcription of the late genes of bacteriophage P2. Suppressor mutations that define the P2 ogr gene o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3541-3548.1990
更新日期:1990-07-01 00:00:00
abstract::The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kina...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.542-547.1982
更新日期:1982-02-01 00:00:00
abstract::Neisseria gonorrhoeae is prone to undergo autolysis under many conditions not conducive to growth. The role of autolysis during gonococcal infection is not known, but possible advantages for the bacterial population include provision of nutrients to a starving population, modulation of the host immune response by rele...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00724-06
更新日期:2006-10-01 00:00:00
abstract::Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.3.1022-1035.2005
更新日期:2005-02-01 00:00:00
abstract::Late log-phase Escherichia coli B/r cells are 1.6 times more sensitive to killing by X rays than are stationary-phase cells when grown in Brain Heart Infusion (BHI) + glucose. The number of single-chain breaks formed per krad is the same for log- and stationary-phase cells. Stationary-phase cells show a somewhat great...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.127-135.1971
更新日期:1971-01-01 00:00:00
abstract::Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.565-571.1987
更新日期:1987-02-01 00:00:00
abstract::The genetics of antibiotic resistance in mutant strains of Streptococcus pyrogenes was studied. Utilizing a type 6 strain (9440) primarily resistant to strepttomycin (Strr), classes of mutant strains were isolated that were resistant to one of the following antibiotics: rifampin (Rifr), erythromycin (Eryr), thiostrept...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.852-859.1978
更新日期:1978-02-01 00:00:00
abstract::Bacterial lipoproteins represent a subset of membrane-associated proteins that are covalently modified with lipids at the N-terminal cysteine. The final step of lipoprotein modification, N-acylation of apolipoproteins, is mediated by apolipoprotein N-acyltransferase (Lnt). Examinations with reconstituted proteoliposom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05013-11
更新日期:2011-09-01 00:00:00
abstract::Escherichia coli WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first few days after plating at densities above 10(8) on plates lacking the required amino acid. They lose viability for some days, and then a subpopulation recovers and there is cell turnover. At very low plating d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2906-2910.1998
更新日期:1998-06-01 00:00:00
abstract::Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750-760. 1961.-More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.5.750-760.1961
更新日期:1961-11-01 00:00:00
abstract::The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host fac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.4071-4074.1997
更新日期:1997-06-01 00:00:00
abstract::Target analyses of the induction of rho(-) mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues afte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1142-1148.1969
更新日期:1969-03-01 00:00:00
abstract::Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activiti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5938-5944.2004
更新日期:2004-09-01 00:00:00
abstract::It has been found that, in Neurospora crassa, arginine synthesized from exogenous citrulline was not as effectively hydrolyzed as exogenous arginine. This was explained by the observed inhibition of arginase in vitro and in vivo by citrulline. The high arginine pool formed from exogenous citrulline feedback inhibits t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.870-877.1972
更新日期:1972-06-01 00:00:00
abstract::The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.9.2695-2700.1996
更新日期:1996-05-01 00:00:00
abstract::Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00932-07
更新日期:2007-10-01 00:00:00
abstract::Azotobacter vinelandii is a bacterium which undergoes a differentiation process leading to the formation of metabolically dormant cysts. During the encystment process, A. vinelandii produces alkylresorcinol lipids (ARs) that replace the membrane phospholipids and are also components of the layers covering the cyst. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01946-12
更新日期:2013-04-01 00:00:00
abstract::The heptapeptide-nucleotide microcin C (McC) targets aspartyl-tRNA synthetase. Upon its entry into a susceptible cell, McC is processed to release a nonhydrolyzable aspartyl-adenylate that inhibits aspartyl-tRNA synthetase, leading to the cessation of translation and cell growth. Here, we surveyed Escherichia coli cel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01956-07
更新日期:2008-04-01 00:00:00
abstract::Neonatal bacterial meningitis continues to be an important cause of mortality and morbidity worldwide. Escherichia coli possessing the K1 capsular polysaccharide is the most common Gram-negative pathogen causing neonatal meningitis. Here we present the complete genome sequence of neonatal meningitis-associated E. coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06284-11
更新日期:2011-12-01 00:00:00
abstract::An RNA polymerase mutant with a single-base-pair change in the rpoC gene affects chromosome initiation control. The mutation, which is recessive, is a G to A transition leading to the substitution of aspartate for glycine at amino acid residue 1033 in the RNA polymerase beta' subunit. The chromosome copy number is inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5200-5206.1991
更新日期:1991-08-01 00:00:00
abstract::The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.7.2528-2532.2006
更新日期:2006-04-01 00:00:00
abstract::The RepA replication protein of plasmid pSC101 binds as a monomer to three repeated sequences (RS1, RS2, and RS3) in the replication origin of the plasmid to initiate duplication and binds as a dimer to two inversely repeated sequences (IR1 and IR2) in its promoter region (D. Manen, L. C. Upegui-Gonzalez, and L. Caro,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.13.4165-4175.1993
更新日期:1993-07-01 00:00:00
abstract::Freeze-etching was applied to preparations, with and without glycerol, of Acinetobacter sp. strain MJT/F5/199A, consisting of intact cells after normal growth or after incubation with chloramphenicol, spheroplasts, and isolated cell walls and outer membranes. Etched preparations show that a regular array of subunits f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.3.1383-1397.1973
更新日期:1973-12-01 00:00:00
abstract::We examined the kinetics of beta-galactosidase (EC 3.2.1.23) induction in the yeast Kluyveromyces lactis. Enzyme activity began to increase 10 to 15 min, about 1/10 of a cell generation, after the addition of inducer and continued to increase linearly for from 7 to 9 cell generations before reaching a maximum, some 12...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.3.777-785.1980
更新日期:1980-06-01 00:00:00
abstract::We purified membrane-bound cytochrome c-550 [cytochrome c-550(m)] to an electrophoretically homogeneous state from Nitrobacter winogradskyi. The cytochrome showed peaks at 409 and 525 nm in the oxidized form and peaks at 416, 521, and 550 nm in the reduced form. The molecular weight of the cytochrome was estimated to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.14.4400-4404.1993
更新日期:1993-07-01 00:00:00
abstract::The CpxA-CpxR two-component signal transduction pathway of Escherichia coli was studied in a mutant (pss-93) lacking phosphatidylethanolamine (PE). Several properties of this mutant are comparable to phenotypes of cpxA point mutants, indicating that this two-component pathway is activated in PE-deficient cells. In con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.4.1029-1034.1997
更新日期:1997-02-01 00:00:00
abstract::S1 nuclease mapping and Northern (RNA) hybridization revealed that the rpoDA gene encoding the principal sigma subunit of Pseudomonas aeruginosa PAO1 is transcribed as a monocistronic mRNA of 2 kb and that the transcription from the rpoDA promoter (PC) starts 32 bases upstream from the first nucleotide of the initiati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.1069-1074.1993
更新日期:1993-02-01 00:00:00
abstract::Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.545-555.1976
更新日期:1976-02-01 00:00:00