Abstract:
:Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide signaling system. When B. subtilis senses DNA damage or high concentrations of potential mating partners that lack the element, ICEBs1 excises from the chromosome and can transfer to recipients. Bacterial conjugation usually requires a DNA relaxase that nicks an origin of transfer (oriT) on the conjugative element and initiates the 5'-to-3' transfer of one strand of the element into recipient cells. The ICEBs1 ydcR (nicK) gene product is homologous to the pT181 family of plasmid DNA relaxases. We found that transfer of ICEBs1 requires nicK and identified a cis-acting oriT that is also required for transfer. Expression of nicK leads to nicking of ICEBs1 between a GC-rich inverted repeat in oriT, and NicK was the only ICEBs1 gene product needed for nicking. NicK likely mediates conjugation of ICEBs1 by nicking at oriT and facilitating the translocation of a single strand of ICEBs1 DNA through a transmembrane conjugation pore.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lee CA,Grossman ADdoi
10.1128/JB.00932-07subject
Has Abstractpub_date
2007-10-01 00:00:00pages
7254-61issue
20eissn
0021-9193issn
1098-5530pii
JB.00932-07journal_volume
189pub_type
杂志文章abstract::Pseudomonas putida DOT-T1E is a solvent-resistant strain that is able to grow in the presence of high concentrations of toluene. We have cloned and sequenced the cti gene of this strain, which encodes the cis/trans isomerase, termed Cti, that catalyzes the cis-trans isomerization of esterified fatty acids in phospholi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.18.5693-5700.1999
更新日期:1999-09-01 00:00:00
abstract::The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.17.5585-5594.1993
更新日期:1993-09-01 00:00:00
abstract::Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and Rz) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Ind...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.354-356.1984
更新日期:1984-04-01 00:00:00
abstract::Antibiotic resistance in Neisseria gonorrhoeae has been associated with the acquisition of R plasmids from heterologous organisms. The broad-host-range plasmids of incompatibility groups P (IncP) and Q (IncQ) have played a role in this genetic exchange in nature. We have utilized derivatives of RSF1010 (IncQ) and RP1 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.18.5793-5799.1991
更新日期:1991-09-01 00:00:00
abstract::The aquatic bacterium Caulobacter crescentus attaches to solid surfaces through an adhesive holdfast located at the tip of its polar stalk, a thin cylindrical extension of the cell membrane. In this paper, the elastic properties of the C. crescentus stalk and holdfast assembly were studied by using video light microsc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.1.257-265.2005
更新日期:2005-01-01 00:00:00
abstract::Genes involved in the biosynthesis of leucine have been mapped in Bacillus megaterium QM B1551, using transducing phage MP13. Mutations were designated leuA, leuB, or leuC on the basis of enzyme assays. Two mutant strains were deficient in the enzyme activities of leuA (alpha-isopropylmalate synthase) and leuC (beta-i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.2.454-459.1984
更新日期:1984-02-01 00:00:00
abstract::Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1566-1573.1975
更新日期:1975-12-01 00:00:00
abstract::Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.867-872.1992
更新日期:1992-02-01 00:00:00
abstract::Pyrazinamide (PZA) is an important antituberculosis drug. Unlike most antibacterial agents, PZA, despite its remarkable in vivo activity, has no activity against Mycobacterium tuberculosis in vitro except at an acidic pH. M. tuberculosis is uniquely susceptible to PZA, but other mycobacteria as well as nonmycobacteria...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.7.2044-2049.1999
更新日期:1999-04-01 00:00:00
abstract::Silicibacter sp. strain TM1040, a member of the Roseobacter clade, forms a symbiosis with unicellular phytoplankton, which is inextricably linked to the biphasic "swim or stick" lifestyle of the bacteria. Mutations in flaC bias the population toward the motile phase. Renewed examination of the FlaC(-) strain (HG1016) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01777-12
更新日期:2013-02-01 00:00:00
abstract::PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.1.119-128.1972
更新日期:1972-07-01 00:00:00
abstract::Antibody in hyperimmune rabbit antisera specific for Staphylococcus aureus teichoic acid was shown to be associated with the IgM fraction. Treatment of such sera with mercaptoethanol destroyed its activity in passive mouse protection tests, whereas absorption with antirabbit IgG had no effect. Antibody response in nor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.5.1540-1545.1968
更新日期:1968-11-01 00:00:00
abstract::Burkholderia pseudomallei is the causative agent of melioidosis, an often fatal infection of humans and animals. The virulence of this pathogen is thought to depend on a number of secreted proteins, including the MprA metalloprotease. We observed that MprA is produced upon entry into the stationary phase, when the cel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.8.2288-2294.2004
更新日期:2004-04-01 00:00:00
abstract::Rhodobacter capsulatus mutants unable to fix nitrogen were isolated by random transposon Tn5 mutagenesis. The Tn5 insertion sites of 30 Nif- mutants were mapped within three unlinked chromosomal regions designated A, B, and C. The majority of Tn5 insertions (21 mutants) map within nif region A, characterized by two Cl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.2.693-699.1988
更新日期:1988-02-01 00:00:00
abstract::Spirochetes are an evolutionary and structurally unique group of bacteria. Outermost is a membrane sheath (OS), and within this sheath are the protoplasmic cell cylinder (PC) and periplasmic flagella (PFs). The PFs are attached at each end of the PC and, depending on the species, may or may not overlap in the center o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.22.6539-6545.1996
更新日期:1996-11-01 00:00:00
abstract::Carbon catabolite repression (CCR) allows bacteria to alter metabolism in response to the availability of specific sugar sources, and increasing evidence suggests that CCR is involved in regulating virulence gene expression in many pathogens. A scan of the M1 SF370 group A streptococcus (GAS) genome using a Bacillus s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01038-07
更新日期:2007-12-01 00:00:00
abstract::Derivatives of Pseudomonas sp. B13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the TOL plasmid of Pseudomonas putida mt-2 were studied. The utilization of these substrates, a property not shared by the parent strains, was shown to dep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.2.467-473.1980
更新日期:1980-05-01 00:00:00
abstract::Groman, Neal B. (University of Washington, Seattle) and Grace Suzuki. Quantitative study of endolysin synthesis during reproduction of lambda phages. J. Bacteriol. 86:187-194. 1963.-Endolysin is presumed to be a phage-induced enzyme participating in lysis through its destructive action on the host cell wall. A method ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.2.187-194.1963
更新日期:1963-08-01 00:00:00
abstract:UNLABELLED:Corynebacterium glutamicum metabolizes sialic acid (Neu5Ac) to fructose-6-phosphate (fructose-6P) via the consecutive activity of the sialic acid importer SiaEFGI, N-acetylneuraminic acid lyase (NanA), N-acetylmannosamine kinase (NanK), N-acetylmannosamine-6P epimerase (NanE), N-acetylglucosamine-6P deacetyl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00820-15
更新日期:2016-07-28 00:00:00
abstract::The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00834-08
更新日期:2008-12-01 00:00:00
abstract::The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5797-5802.1996
更新日期:1996-10-01 00:00:00
abstract::A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynami...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.21.6056-6059.2002
更新日期:2002-11-01 00:00:00
abstract::Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from hu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00222-09
更新日期:2009-06-01 00:00:00
abstract::We isolated an unusual mutant in which the arabinose operon carried an araC lesion that led to a dominant fucose resistance while retaining recessive constitutivity. All previously characterized C(c) mutations have been recessive for both fucose resistance and constitutivity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.2.711-713.1973
更新日期:1973-08-01 00:00:00
abstract::A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.2.668-670.1976
更新日期:1976-11-01 00:00:00
abstract::The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2197-2204.1993
更新日期:1993-04-01 00:00:00
abstract::The type III secretion system (T3SS) of Pseudomonas aeruginosa is an important virulence factor. The T3SS of P. aeruginosa can be induced by a low calcium signal or upon direct contact with the host cells. The exact pathway of signal sensing and T3SS activation is not clear. By screening a transposon insertion mutant ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7575-7585.2004
更新日期:2004-11-01 00:00:00
abstract::We have isolated mutations that block sporulation after formation of the polar septum in Bacillus subtilis. These mutations were mapped to the two genes of a new locus, spoIIS. Inactivation of the second gene, spoIISB, decreases sporulation efficiency by 4 orders of magnitude. Inactivation of the first gene, spoIISA, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.12.3574-3581.2001
更新日期:2001-06-01 00:00:00
abstract::The colicinogenic B factor, transferred from Escherichia coli strain K77 (and termed ColB2-K77 or ColB2) to an E. coli K12 F(-) strain, is capable of promoting its own transfer to other K12 F(-) strains at a low rate (from LFC cultures) which can be increased under special conditions (HFC cultures). LFC cultures of K1...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.3.900-906.1971
更新日期:1971-09-01 00:00:00
abstract::Hedgecock, L. W. (Veterans Administration Hospital, Kansas City, Mo.) and R. L. Costello. Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bacteriol. 84:195-205. 1962-The ability of mycobacteria to utilize nitrate as a sole source of nitrogen was examined. Nitrate-nitrogen was readily utilized by ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.195-205.1962
更新日期:1962-08-01 00:00:00