Abnormal excision and transfer of chromosomal segments by a strain of Escherichia coli K-12.

Abstract:

:PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the excision and transfer of extended chromosomal segments which include the integrated F-gal; the excised segments are inferred to have circularized. Their excision, which is independent of the recA(+) allele, occurs at an unusually high rate during conjugation; a mutant F-initiator protein is suggested as the cause of this phenomenon. After their establishment in recipients, the enlarged F-genotes undergo further deletions of included donor genes by a process which is again recA(+)-independent. In Rec(+), but not in Rec(-), cells, a high proportion of the deleted fragments are rescued by integration into the recipient's chromosome.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bergquist PL,Adelberg EA

doi

10.1128/JB.111.1.119-128.1972

subject

Has Abstract

pub_date

1972-07-01 00:00:00

pages

119-28

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

111

pub_type

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