A novel activity in Escherichia coli K-12 that directs restriction of DNA modified at CG dinucleotides.

Abstract:

:The restriction systems McrA and McrB of Escherichia coli K-12 are known to attack DNA containing modified cytosine. In strains lacking both activities, however, we observed that DNA methylated at CG dinucleotides (as is mammalian DNA) was still significantly restricted. We show that this substantial barrier to the acceptance of 5-methylcytosine-containing DNA is attributable to a hitherto unknown activity of the Mrr restriction system. Strikingly, the multiple systems used by this gut inhabitant to determine the fate of invading DNA will all limit genetic exchange with its mammalian host(s), reinforcing the idea that one role of DNA methylation is to serve as a "molecular passport" (E. A. Raleigh, R. Trimarchi, and H. Revel, Genetics 122:279-296, 1989).

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kelleher JE,Raleigh EA

doi

10.1128/jb.173.16.5220-5223.1991

subject

Has Abstract

pub_date

1991-08-01 00:00:00

pages

5220-3

issue

16

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

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