Conservation of plasmid maintenance functions between linear and circular plasmids in Borrelia burgdorferi.

Abstract:

:The Lyme disease agent Borrelia burgdorferi maintains both linear and circular plasmids that appear to be essential for mammalian infection. Recent studies have characterized the circular plasmid regions that confer autonomous replication, but the genetic elements necessary for linear plasmid maintenance have not been experimentally identified. Two vectors derived from linear plasmids lp25 and lp28-1 were constructed and shown to replicate autonomously in B. burgdorferi. These vectors identify internal regions of linear plasmids necessary for autonomous replication in B. burgdorferi. Although derived from linear plasmids, the vectors are maintained in circular form in B. burgdorferi, indicating that plasmid maintenance functions are conserved, regardless of DNA form. Finally, derivatives of these vectors indicate that paralogous gene family 49 is apparently not required for either circular or linear plasmid replication.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Stewart PE,Chaconas G,Rosa P

doi

10.1128/jb.185.10.3202-3209.2003

keywords:

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

3202-9

issue

10

eissn

0021-9193

issn

1098-5530

journal_volume

185

pub_type

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