Inhibition of RNA synthesis by oxolinic acid is unrelated to average DNA supercoiling.

abstract：:Heme A, as a prosthetic group, is found exclusively in respiratory oxidases of mitochondria and aerobic bacteria. Bacillus subtilis CtaA and other heme A synthases catalyze the conversion of a methyl side group on heme O into a formyl group. The catalytic mechanism of heme A synthase is not understood, and little is k...

journal_title：Journal of bacteriology

authors： Hederstedt L,Lewin A,Throne-Holst M

更新日期：2005-12-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has been used to amplify DNA fragments by using eucaryotic genomic DNA as a template. We show that bacterial genomic DNA can be used as a template for PCR amplification. We demonstrate that DNA fragments at least as large as 4,400 base pairs can be amplified with fidelity and that t...

journal_title：Journal of bacteriology

authors： Shyamala V,Ames GF

更新日期：1989-03-01 00:00:00

abstract：:Two bacteriophage BF23 late genes, genes 24 and 25, were isolated on a 7.4-kb PstI fragment from the phage DNA, and their nucleotide sequences were determined. Gene 24 encodes a minor tail protein with the expected M(r) of 34,309, and gene 25 located 4 bp upstream of gene 24 encodes a major tail protein with the expec...

journal_title：Journal of bacteriology

authors： Nakayama S,Kaneko T,Ishimaru H,Moriwaki H,Mizobuchi K

更新日期：1994-12-01 00:00:00

abstract：:Biogenesis of the outer membrane (OM) is an essential process in gram-negative bacteria. One of the key steps of OM biogenesis is the assembly of integral outer membrane beta-barrel proteins (OMPs) by a protein machine called the Bam complex. In Escherichia coli, the Bam complex is composed of the essential proteins B...

journal_title：Journal of bacteriology

authors： Rigel NW,Schwalm J,Ricci DP,Silhavy TJ

更新日期：2012-03-01 00:00:00

abstract：:Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...

journal_title：Journal of bacteriology

authors： Walderich B,Ursinus-Wössner A,van Duin J,Höltje JV

更新日期：1988-11-01 00:00:00

abstract：:In the photosynthetic bacterium Rhodobacter capsulatus, the enzyme DNA gyrase has been implicated in the expression of genes for anaerobic metabolic processes such as nitrogen fixation and photosynthesis. To assess the involvement of supercoiling in anaerobic gene expression, we have developed an assay to detect in vi...

journal_title：Journal of bacteriology

authors： Cook DN,Armstrong GA,Hearst JE

更新日期：1989-09-01 00:00:00

abstract：:Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...

journal_title：Journal of bacteriology

authors： Jürgens UJ,Weckesser J

更新日期：1985-10-01 00:00:00

abstract：:A noncomposite transposon, designated Tn5541, was isolated from strain Fd33 of the filamentous cyanobacterium Fremyella diplosiphon UTEX 481. Sequence analysis showed that Tn5541 is structurally and genetically very similar to Tn5469, which is also endogenous to F. diplosiphon. Both Tn5469 and Tn5541 encode homologous...

journal_title：Journal of bacteriology

authors： Schaefer MR,Kahn K

更新日期：1998-11-01 00:00:00

abstract：:Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...

journal_title：Journal of bacteriology

authors： Friedberg D,Platko JV,Tyler B,Calvo JM

更新日期：1995-03-01 00:00:00

abstract：:In aquatic environments, Caulobacter spp. can be found at the boundary between liquid and air known as the neuston. I report an approach to study temporal features of Caulobacter crescentus colonization and pellicle biofilm development at the air-liquid interface and have defined the role of cell surface structures in...

journal_title：Journal of bacteriology

authors： Fiebig A

更新日期：2019-08-22 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

journal_title：Journal of bacteriology

authors： Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

更新日期：2010-04-01 00:00:00

abstract：:Acid hydrolysates of 140 strains, representing 11 species of the genus Bacteroides, were analyzed by capillary gas-liquid chromatography for total cellular fatty acid. All samples contained components which appeared to be hydroxy fatty acid. The relative amount and chain length distribution of the hydroxy fatty acids,...

journal_title：Journal of bacteriology

authors： Mayberry WR

更新日期：1980-08-01 00:00:00

abstract：:Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA m...

journal_title：Journal of bacteriology

authors： Thanedar S,Dineshkumar TK,Varshney U

更新日期：2001-12-01 00:00:00

abstract：:A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...

journal_title：Journal of bacteriology

authors： Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

更新日期：1987-10-01 00:00:00

abstract：:A naturally occurring constitutive macrolide-lincosamide-streptogramin B (MLS) resistance plasmid, pNE131, from Staphylococcus epidermidis was chosen to study the molecular basis of constitutive expression. Restriction and functional maps of pNE131 are presented along with the nucleotide sequence of ermM, the gene whi...

journal_title：Journal of bacteriology

authors： Lampson BC,Parisi JT

更新日期：1986-05-01 00:00:00

abstract：:To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this ...

journal_title：Journal of bacteriology

authors： Hatfull GF,Joyce CM

更新日期：1986-06-01 00:00:00

abstract：:Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

journal_title：Journal of bacteriology

authors： Huang AM,Lee JI,King SC,Wilson TH

更新日期：1992-08-01 00:00:00

abstract：:An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a mo...

journal_title：Journal of bacteriology

authors： Lackey D,Walker GC,Keng T,Linn S

更新日期：1977-08-01 00:00:00

abstract：:LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...

journal_title：Journal of bacteriology

authors： Bowers CW,Lau F,Silhavy TJ

更新日期：2003-10-01 00:00:00

abstract：:A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1973-05-01 00:00:00

abstract：:The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...

journal_title：Journal of bacteriology

authors： Rodriguez RL,Davern CI

更新日期：1976-01-01 00:00:00

abstract：:The role of the carboxy terminus of the Escherichia coli cell division protein FtsA in bacterial division has been studied by making a series of short sequential deletions spanning from residue 394 to 420. Deletions as short as 5 residues destroy the biological function of the protein. Residue W415 is essential for th...

journal_title：Journal of bacteriology

authors： Yim L,Vandenbussche G,Mingorance J,Rueda S,Casanova M,Ruysschaert JM,Vicente M

更新日期：2000-11-01 00:00:00

abstract：:The isolation and properties of a mutant dependent upon exogenous carbamyl aspartate for resistance to 5-fluorouracil are described. The mutant was deficient in the synthesis of ubiquinone and accumulated a quinone provisionally identified as the ubiquinone precursor 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. T...

journal_title：Journal of bacteriology

authors： Zak VL,Kelln RA

更新日期：1981-02-01 00:00:00

abstract：:Hancock, R. (Harvard Medical School, Boston, Mass.). Early effects of streptomycin on Bacillus megaterium. J. Bacteriol. 88:633-639. 1964.-The sequence of some early effects of streptomycin on Bacillus megaterium was investigated. An increase in the rate of efflux of cellular K ions preceded loss of viability, but eff...

journal_title：Journal of bacteriology

authors： HANCOCK R

更新日期：1964-09-01 00:00:00

abstract：:The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...

journal_title：Journal of bacteriology

authors： Terrak M,Nguyen-Distèche M

更新日期：2006-04-01 00:00:00

abstract：:A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by a...

journal_title：Journal of bacteriology

authors： Gratzner H,Sheehan DN

更新日期：1969-02-01 00:00:00

abstract：:Apparent pK values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at pH 7 in media free from nitrogen, sulfur, and phosphorus. Of the compounds possessing pK' values near pH 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (EMTA) was c...

journal_title：Journal of bacteriology

authors： Mallette MF

更新日期：1967-08-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00

abstract：:The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...

journal_title：Journal of bacteriology

authors： Gyohda A,Funayama N,Komano T

更新日期：1997-03-01 00:00:00

abstract：:We have investigated the evolutionary relationships between two facultatively anaerobic Magnetospirillum strains (AMB-1 and MGT-1) and fastidious, obligately microaerophilic species, such as Magnetospirillum magnetotacticum, using a molecular phylogenetic approach. Genomic DNA from strains MGT-1 and AMB-1 was used as ...

journal_title：Journal of bacteriology

authors： Burgess JG,Kawaguchi R,Sakaguchi T,Thornhill RH,Matsunaga T

更新日期：1993-10-01 00:00:00