Crystal structure of the cystic fibrosis transmembrane conductance regulator inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor.

Abstract:

:Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its sequence violates two strictly conserved EH motifs and also is compatible with other alpha/beta hydrolase family members with diverse substrate specificities. To investigate the mechanistic basis of Cif activity, we have determined its structure at 1.8-A resolution by X-ray crystallography. The catalytic triad consists of residues Asp129, His297, and Glu153, which are conserved across the family of EHs. At other positions, sequence deviations from canonical EH active-site motifs are stereochemically conservative. Furthermore, detailed enzymatic analysis confirms that Cif catalyzes the hydrolysis of epoxide compounds, with specific activity against both epibromohydrin and cis-stilbene oxide, but with a relatively narrow range of substrate selectivity. Although closely related to two other classes of alpha/beta hydrolase in both sequence and structure, Cif does not exhibit activity as either a haloacetate dehalogenase or a haloalkane dehalogenase. A reassessment of the structural and functional consequences of the H269A mutation suggests that Cif's effect on host-cell CFTR expression requires the hydrolysis of an extended endogenous epoxide substrate.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

doi

10.1128/JB.01348-09

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

1785-95

issue

7

eissn

0021-9193

issn

1098-5530

pii

JB.01348-09

journal_volume

192

pub_type

杂志文章
  • Genetic analysis of the tetA(C) gene on plasmid pBR322.

    abstract::The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell. The tetA(C) gene was mutagenized with hydroxylamine, and missense mutations causing the loss of tetracycline resistance we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.24.7926-7933.1992

    authors: McNicholas P,Chopra I,Rothstein DM

    更新日期:1992-12-01 00:00:00

  • Dinitrogenase reductase-activating glycohydrolase can be released from chromatophores of Rhodospirillum rubrum by treatment with MgGDP.

    abstract::Dinitrogenase reductase-activating glycohydrolase (DRAG), involved in the regulation of nitrogenase activity in Rhodospirillum rubrum, is associated with chromatophore membranes in cell extracts. We show that DRAG can be specifically released by treatment with MgGDP; other nucleotides studied had no effect. The DRAG a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.24.7872-7874.1997

    authors: Norén A,Nordlund S

    更新日期:1997-12-01 00:00:00

  • The YSIRK-G/S motif of staphylococcal protein A and its role in efficiency of signal peptide processing.

    abstract::Many surface proteins of pathogenic gram-positive bacteria are linked to the cell wall envelope by a mechanism requiring a C-terminal sorting signal with an LPXTG motif. Surface proteins of Streptococcus pneumoniae harbor another motif, YSIRK-G/S, which is positioned within signal peptides. The signal peptides of some...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.9.2910-2919.2003

    authors: Bae T,Schneewind O

    更新日期:2003-05-01 00:00:00

  • Proteomic characterization of Yersinia pestis virulence.

    abstract::The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.23.8172-8180.2005

    authors: Chromy BA,Choi MW,Murphy GA,Gonzales AD,Corzett CH,Chang BC,Fitch JP,McCutchen-Maloney SL

    更新日期:2005-12-01 00:00:00

  • Dependence of Escherichia coli hyperbaric oxygen toxicity on the lipid acyl chain composition.

    abstract::This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.3.808-820.1978

    authors: Harley JB,Santangelo GM,Rasmussen H,Goldfine H

    更新日期:1978-06-01 00:00:00

  • Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp. Strain PCC 7120.

    abstract::HetR plays a key role in regulation of heterocyst differentiation and patterning in Anabaena It directly regulates genes involved in heterocyst differentiation (such as hetP and hetZ), genes involved in pattern formation (patA), and many others. In this study, we investigated the functional relationship of hetP and he...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00707-17

    authors: Zhang H,Wang S,Wang Y,Xu X

    更新日期:2018-04-09 00:00:00

  • CTX prophages in classical biotype Vibrio cholerae: functional phage genes but dysfunctional phage genomes.

    abstract::CTXphi is a filamentous, lysogenic bacteriophage whose genome encodes cholera toxin, the primary virulence factor produced by Vibrio cholerae. CTX prophages in O1 El Tor and O139 strains of V. cholerae are found within arrays of genetically related elements integrated at a single locus within the V. cholerae large chr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.24.6992-6998.2000

    authors: Davis BM,Moyer KE,Boyd EF,Waldor MK

    更新日期:2000-12-01 00:00:00

  • Genomic insights into the convergence and pathogenicity factors of Campylobacter jejuni and Campylobacter coli species.

    abstract::Whether or not bacteria form coherent evolutionary groups via means of genetic exchange and, hence, elicit distinct species boundaries remains an unsettled issue. A recent report implied that not only may the former be true but also, in fact, the clearly distinct Campylobacter jejuni and Campylobacter coli species may...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00519-09

    authors: Caro-Quintero A,Rodriguez-Castaño GP,Konstantinidis KT

    更新日期:2009-09-01 00:00:00

  • Genome-based analysis and gene dosage studies provide new insight into 3-hydroxy-4-methylvalerate biosynthesis in Ralstonia eutropha.

    abstract::Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02474-14

    authors: Saika A,Ushimaru K,Mizuno S,Tsuge T

    更新日期:2015-04-01 00:00:00

  • The pbgPE operon in Photorhabdus luminescens is required for pathogenicity and symbiosis.

    abstract::Photorhabdus is a genus of gram-negative Enterobacteriaceae that is pathogenic to insect larvae while also maintaining a mutualistic relationship with nematodes from the family Heterorhabditis, where the bacteria occupy the gut of the infective juvenile (IJ) stage of the nematode. In this study we describe the identif...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.77-84.2005

    authors: Bennett HP,Clarke DJ

    更新日期:2005-01-01 00:00:00

  • Long-chain fatty acid inhibition of growth of Streptococcus agalactiae in a chemically defined medium.

    abstract::Willett, Norman P. (University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pa.), and Guy E. Morse. Long-chain fatty acid inhibition of growth of Streptococcus agalactiae in a chemically defined medium. J. Bacteriol. 91:2245-2250. 1966.-A chemically defined medium was developed for Streptococcus agal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.6.2245-2250.1966

    authors: Willett NP,Morse GE

    更新日期:1966-06-01 00:00:00

  • Crystal structure of pyridoxal kinase from the Escherichia coli pdxK gene: implications for the classification of pyridoxal kinases.

    abstract::The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00122-06

    authors: Safo MK,Musayev FN,di Salvo ML,Hunt S,Claude JB,Schirch V

    更新日期:2006-06-01 00:00:00

  • Catabolite repression of Escherichia coli biofilm formation.

    abstract::Biofilm formation was repressed by glucose in several species of Enterobacteriaceae. In Escherichia coli, this effect was mediated at least in part by cyclic AMP (cAMP)-cAMP receptor protein. A temporal role for cAMP in biofilm development was indicated by the finding that glucose addition after approximately 24 h fai...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.12.3406-3410.2002

    authors: Jackson DW,Simecka JW,Romeo T

    更新日期:2002-06-01 00:00:00

  • Identification of a single amino acid substitution in the diphtheria toxin A chain of CRM 228 responsible for the loss of enzymatic activity.

    abstract::CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.15.4766-4769.1994

    authors: Johnson VG,Nicholls PJ

    更新日期:1994-08-01 00:00:00

  • Gene transfer in magnetic bacteria: transposon mutagenesis and cloning of genomic DNA fragments required for magnetosome synthesis.

    abstract::Broad-host-range IncP and IncQ plasmids have been transferred to the aerobic magnetic bacterium Aquaspirillum sp. strain AMB-1. Conjugal matings with Escherichia coli S17-1 allowed high-frequency transfer of the RK2 derivative pRK415 (4.5 x 10(-3) transconjugant per recipient cell) and the RSF1010 derivative pKT230 (3...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.9.2748-2753.1992

    authors: Matsunaga T,Nakamura C,Burgess JG,Sode K

    更新日期:1992-05-01 00:00:00

  • The Helicobacter pylori CrdRS two-component regulation system (HP1364/HP1365) is required for copper-mediated induction of the copper resistance determinant CrdA.

    abstract::Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked cop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.13.4683-4688.2005

    authors: Waidner B,Melchers K,Stähler FN,Kist M,Bereswill S

    更新日期:2005-07-01 00:00:00

  • Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro.

    abstract::The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5797-5802.1996

    authors: Metcalf WW,Zhang JK,Shi X,Wolfe RS

    更新日期:1996-10-01 00:00:00

  • Involvement of the cis/trans isomerase Cti in solvent resistance of Pseudomonas putida DOT-T1E.

    abstract::Pseudomonas putida DOT-T1E is a solvent-resistant strain that is able to grow in the presence of high concentrations of toluene. We have cloned and sequenced the cti gene of this strain, which encodes the cis/trans isomerase, termed Cti, that catalyzes the cis-trans isomerization of esterified fatty acids in phospholi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.18.5693-5700.1999

    authors: Junker F,Ramos JL

    更新日期:1999-09-01 00:00:00

  • Identification and characterization of a novel member of the radical AdoMet enzyme superfamily and implications for the biosynthesis of the Hmd hydrogenase active site cofactor.

    abstract::The genetic context, phylogeny, and biochemistry of a gene flanking the H(2)-forming methylene-H(4)-methanopterin dehydrogenase gene (hmdA), here designated hmdB, indicate that it is a new member of the radical S-adenosylmethionine enzyme superfamily. In contrast to the characteristic CX(3)CX(2)C or CX(2)CX(4)C motif ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01125-09

    authors: McGlynn SE,Boyd ES,Shepard EM,Lange RK,Gerlach R,Broderick JB,Peters JW

    更新日期:2010-01-01 00:00:00

  • Uranyl nitrate inhibition of transport systems in Saccharomyces cerevisiae.

    abstract::Uranyl nitrate inhibited the transport of several amino acids, a vitamin, and a disaccharide into yeast. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.1205-1206.1971

    authors: Maxwell WA,Metzler R,Spoerl E

    更新日期:1971-03-01 00:00:00

  • Active subunits of Escherichia coli glutamate synthase.

    abstract::The large and small subunits of Escherichia coli glutamate synthase were isolated. The small subunit catalyzes the NH(3)-dependent synthesis of glutamate. The large subunit exhibits glutaminase activity. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.1.539-541.1976

    authors: Mantsala P,Zalkin H

    更新日期:1976-04-01 00:00:00

  • Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic di-GMP levels, and enhanced dispersal.

    abstract::Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00975-09

    authors: Barraud N,Schleheck D,Klebensberger J,Webb JS,Hassett DJ,Rice SA,Kjelleberg S

    更新日期:2009-12-01 00:00:00

  • Respiratory metabolism of a "petite negative"yeast Schizosaccharomyces pombe 972h-.

    abstract::The respiratory metabolism of Schizosaccharomyces pombe 972h(-), a fission, haplontic, "petite negative" yeast, was studied. Glucose and glycerol are good growth substrates and are oxidized under appropriate conditions. l-Lactate, ethanol, malate, and succinate are oxidized but are poor substrates for growth. d-Lactat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.473-481.1970

    authors: Heslot H,Goffeau A,Louis C

    更新日期:1970-10-01 00:00:00

  • Initiation of chromosome replication in bacteria: analysis of an inhibitor control model.

    abstract::This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constitue...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.5231-5240.1987

    authors: Margalit H,Grover NB

    更新日期:1987-11-01 00:00:00

  • A Genome-Wide Helicobacter pylori Morphology Screen Uncovers a Membrane-Spanning Helical Cell Shape Complex.

    abstract::Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00724-18

    authors: Yang DC,Blair KM,Taylor JA,Petersen TW,Sessler T,Tull CM,Leverich CK,Collar AL,Wyckoff TJ,Biboy J,Vollmer W,Salama NR

    更新日期:2019-06-21 00:00:00

  • The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli.

    abstract::Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.24.7397-7402.2001

    authors: Thanedar S,Dineshkumar TK,Varshney U

    更新日期:2001-12-01 00:00:00

  • mgpS, a complex regulatory locus involved in the transcriptional control of the puc and puf operons in Rhodobacter sphaeroides 2.4.1.

    abstract::A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.1.35-45.1996

    authors: Sabaty M,Kaplan S

    更新日期:1996-01-01 00:00:00

  • Physiological function of superoxide dismutase in glucose-limited chemostat cultures of Escherichia coli.

    abstract::Conditions for continuous culture of Escherichia coli K-12 His- Thi- under glucose limitation were established. Both the capacity for respiration, at D greater than 0.2/h, and specific activity of superoxide dismutase increased as a function of specific growth rate, whereas peroxidase and catalase were either invarian...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.805-811.1977

    authors: Hassan HM,Fridovich I

    更新日期:1977-05-01 00:00:00

  • Role and regulation of Bacillus subtilis glutamate dehydrogenase genes.

    abstract::The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Belitsky BR,Sonenshein AL

    更新日期:1998-12-01 00:00:00

  • cse, a Chimeric and variable gene, encodes an extracellular protein involved in cellular segregation in Streptococcus thermophilus.

    abstract::The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.8.2737-2746.2005

    authors: Borges F,Layec S,Thibessard A,Fernandez A,Gintz B,Hols P,Decaris B,Leblond-Bourget N

    更新日期:2005-04-01 00:00:00