A Salmonella typhimurium mutant dependent upon carbamyl aspartate for resistance to 5-fluorouracil is specifically affected in ubiquinone biosynthesis.

Abstract:

:The isolation and properties of a mutant dependent upon exogenous carbamyl aspartate for resistance to 5-fluorouracil are described. The mutant was deficient in the synthesis of ubiquinone and accumulated a quinone provisionally identified as the ubiquinone precursor 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. The mutation resulted in an alteration in the regulation of synthesis of enzymes involved in de novo pyrimidine biosynthesis but did not establish a functional block in dihydroorotate dehydrogenase activity in vivo. Conditional resistance to 5-fluorouracil apparently occurred through an inhibition of the conversion of the analog to the nucleotide level.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Zak VL,Kelln RA

doi

10.1128/JB.145.2.1095-1098.1981

subject

Has Abstract

pub_date

1981-02-01 00:00:00

pages

1095-8

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

145

pub_type

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