GLYCINE SYNTHESIS AND METABOLISM IN ESCHERICHIA COLI.

abstract：:Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...

journal_title：Journal of bacteriology

authors： Pi J,Wookey PJ,Pittard AJ

更新日期：1993-11-01 00:00:00

abstract：:A proline analogue, 4,5-dehydro-l-pipecolic acid (baikiain) induces the formation in Salmonella typhimurium of the two enzymes catalyzing the degradation of proline, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid (P5C) dehydrogenase. The level of induction by 20 mm baikiain is about 10% of the maximum level ...

journal_title：Journal of bacteriology

authors： Dendinger S,Brill WJ

更新日期：1972-12-01 00:00:00

abstract：:Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Ald protein sequences (M. J. Ward, H. Lew, A. Treuner-Lange, and D. R. Zusman, J. Bacteriol. 180:5668-5675, 1998). In this study, we have confirmed that aldA ...

journal_title：Journal of bacteriology

authors： Ward MJ,Lew H,Zusman DR

更新日期：2000-01-01 00:00:00

abstract：:Alginate-producing (mucoid) strains of Pseudomonas aeruginosa possess a 54-kDa outer membrane (OM) protein (AlgE) which is missing in nonmucoid bacteria. The coding region of the algE gene from mucoid P. aeruginosa CF3/M1 was subcloned in the expression vector pT7-7 and expressed in Escherichia coli. The level of expr...

journal_title：Journal of bacteriology

authors： Rehm BH,Boheim G,Tommassen J,Winkler UK

更新日期：1994-09-01 00:00:00

abstract：:Kasugamycin-resistant mutants of Bacillus subtilis were isolated and classified into two groups, one of which had resistance to kasugamycin in in vitro protein synthesis and mapped in the ribosomal region. The other group had no resistance to kasugamycin in in vitro protein synthesis and had weak cross-resistance to g...

journal_title：Journal of bacteriology

authors： Tominaga A,Kobayashi Y

更新日期：1978-09-01 00:00:00

abstract：:Bacteria have evolved numerous means of survival in adverse environments with dormancy, as represented by "persistence" and the "viable but nonculturable" (VBNC) state, now recognized to be common modes for such survival. VBNC cells have been defined as cells which, induced by some stress, become nonculturable on medi...

journal_title：Journal of bacteriology

authors： Ayrapetyan M,Williams T,Oliver JD

更新日期：2018-09-24 00:00:00

abstract：:The sets of compounds that can support growth of an organism are defined by the presence of transporters and metabolic pathways that convert nutrient sources into cellular components and energy for growth. A collection of known nutrient sources can therefore serve both as an impetus for investigating new metabolic pat...

journal_title：Journal of bacteriology

authors： Mackie A,Paley S,Keseler IM,Shearer A,Paulsen IT,Karp PD

更新日期：2014-03-01 00:00:00

abstract：:The gene encoding the fimbrial subunit protein of Bacteroides gingivalis 381, fimbrilin, has been cloned and sequenced. The gene was present as a single copy on the bacterial chromosome, and the codon usage in the gene conformed closely to that expected for an abundant protein. The predicted size of the mature protein...

journal_title：Journal of bacteriology

authors： Dickinson DP,Kubiniec MA,Yoshimura F,Genco RJ

更新日期：1988-04-01 00:00:00

abstract：:Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...

journal_title：Journal of bacteriology

authors： Tsai HF,Chang YC,Washburn RG,Wheeler MH,Kwon-Chung KJ

更新日期：1998-06-01 00:00:00

abstract：:The nucleotide sequence of the Escherichia coli colicin I receptor gene (cir) has been determined. The predicted mature protein consists of 599 amino acids and has a molecular weight of 67,169. Several previously noted characteristics of other E. coli outer membrane protein sequences were also identified in the sequen...

journal_title：Journal of bacteriology

authors： Nau CD,Konisky J

更新日期：1989-02-01 00:00:00

abstract：:Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...

journal_title：Journal of bacteriology

authors： Chaffin DO,Beres SB,Yim HH,Rubens CE

更新日期：2000-08-01 00:00:00

abstract：:A sequenced collection of plasmid-borne random fusions of Escherichia coli DNA to a Photorhabdus luminescens luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in E. coli. Hi...

journal_title：Journal of bacteriology

authors： Van Dyk TK,DeRose EJ,Gonye GE

更新日期：2001-10-01 00:00:00

abstract：:Isolation of six strains of Escherichia coli K-12 carrying mutations affecting the citrate-dependent iron uptake system is described. Genetic analysis of these mutants showed that the mutation affecting the citrate system are cluster together at about min 6 on the E. coli chromosome. ...

journal_title：Journal of bacteriology

authors： Woodrow GC,Langman L,Young IG,Gibson F

更新日期：1978-03-01 00:00:00

abstract：:Hg2+-resistant mutants were isolated from Saccharomyces cerevisiae. Although they were very much like the parental strains in terms of colony-forming ability, they grew faster than the parental strains in the presence of sublethal doses of Hg2+. The Hg2+-resistant mutations were dominant. They were centromere linked a...

journal_title：Journal of bacteriology

authors： Ono B,Ohue H,Ishihara F

更新日期：1988-12-01 00:00:00

abstract：:The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region...

journal_title：Journal of bacteriology

authors： Hartmann RK,Toschka HY,Erdmann VA

更新日期：1991-04-01 00:00:00

abstract：:We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...

journal_title：Journal of bacteriology

authors： Perez-Casal JF,Gammie AE,Crosa JH

更新日期：1989-04-01 00:00:00

abstract：:The lining of the maltodextrin-specific maltoporin (LamB) channel exhibits a string of aromatic residues, the greasy slide, part of which has been shown previously by crystallography to be involved in substrate binding. To probe the functional role of the greasy slide, alanine scanning mutagenesis has been performed o...

journal_title：Journal of bacteriology

authors： Van Gelder P,Dumas F,Bartoldus I,Saint N,Prilipov A,Winterhalter M,Wang Y,Philippsen A,Rosenbusch JP,Schirmer T

更新日期：2002-06-01 00:00:00

abstract：:A Rhodobacter capsulatus hemC mutant has been isolated and used to show that oxygen regulates the intracellular levels of porphobilinogen. Experiments using a hemB-cat gene fusion demonstrated that oxygen does not transcriptionally regulate hemB transcription. Porphobilinogen synthase activity is not regulated by oxyg...

journal_title：Journal of bacteriology

authors： Biel AJ,Canada K,Huang D,Indest K,Sullivan K

更新日期：2002-03-01 00:00:00

abstract：:Mycobacterial genomes are endowed with many eukaryote-like nucleotide cyclase genes encoding proteins that can synthesize 3',5'-cyclic AMP (cAMP). However, the roles of cAMP and the need for such redundancy in terms of adenylyl cyclase genes remain unknown. We measured cAMP levels in Mycobacterium smegmatis during gro...

journal_title：Journal of bacteriology

authors： Dass BK,Sharma R,Shenoy AR,Mattoo R,Visweswariah SS

更新日期：2008-06-01 00:00:00

abstract：:The pattern of peptidoglycan (murein) segregation in cells of Escherichia coli with impaired activity of the morphogenetic proteins penicillin-binding protein 2 and RodA has been investigated by the D-cysteine-biotin immunolabeling technique (M. A. de Pedro, J. C. Quintela, J.-V. Höltje, and H. Schwarz, J. Bacteriol. ...

journal_title：Journal of bacteriology

authors： de Pedro MA,Donachie WD,Höltje JV,Schwarz H

更新日期：2001-07-01 00:00:00

abstract：:The glnB gene from Bradyrhizobium japonicum, the endosymbiont of soybeans (Glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. The B. japonicum glnB gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnB gene product...

journal_title：Journal of bacteriology

authors： Martin GB,Thomashow MF,Chelm BK

更新日期：1989-10-01 00:00:00

abstract：:The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effect...

journal_title：Journal of bacteriology

authors： Magnusson LU,Gummesson B,Joksimović P,Farewell A,Nyström T

更新日期：2007-07-01 00:00:00

abstract：:Ornithine carbamoyltransferase synthesis is subject to two major regulatory systems in Saccharomyces cerevisiae. One system is specific for the arginine biosynthetic enzymes, whereas the other appears to be general, acting on a variety of other amino acid pathways as well. We observed that the synthetic capacity for c...

journal_title：Journal of bacteriology

authors： Messenguy F,Cooper TG

更新日期：1977-06-01 00:00:00

abstract：:Pleiotropic effects of two ribosomal mutations for cycloheximide resistance were studied in a double-resistant homocaryon. The results obtained indicated that the combination of the two ribosomal mutations results in: (i) morphological abnormalities which suggest a severe distortion in the extension of cell walls and ...

journal_title：Journal of bacteriology

authors： Vomvoyanni VE,Argyrakis MP

更新日期：1979-08-01 00:00:00

abstract：:Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...

journal_title：Journal of bacteriology

authors： Smith CJ,Spiegel H

更新日期：1987-08-01 00:00:00

abstract：:The iron-molybdenum cofactor (FeMo-co) of nitrogenase contains molybdenum, iron, sulfur, and homocitrate in a ratio of 1:7:9:1. In vitro synthesis of FeMo-co has been established, and the reaction requires an ATP-regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabolic product of Ni...

journal_title：Journal of bacteriology

authors： Shah VK,Rangaraj P,Chatterjee R,Allen RM,Roll JT,Roberts GP,Ludden PW

更新日期：1999-05-01 00:00:00

abstract：:The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S...

journal_title：Journal of bacteriology

authors： Borges F,Layec S,Thibessard A,Fernandez A,Gintz B,Hols P,Decaris B,Leblond-Bourget N

更新日期：2005-04-01 00:00:00

abstract：:A Pseudomonas strain produced an enzyme capable of deacylating 7 beta-(4-carboxybutanamido)cephalosporanic acid to 7-aminocephalosporanic acid in response to glutaric acid. The gene for the enzyme was cloned within the PstI site of pBR325 as a 7.35-kilobase-pair DNA segment from a mutant of this strain whose enzyme is...

journal_title：Journal of bacteriology

authors： Matsuda A,Komatsu KI

更新日期：1985-09-01 00:00:00

abstract：:Trichloroethylene (TCE) is the most frequently detected groundwater contaminant, and 1-naphthol is an important chemical manufacturing intermediate. Directed evolution was used to increase the activity of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 for both chlorinated ethenes and naphthalene oxidatio...

journal_title：Journal of bacteriology

authors： Canada KA,Iwashita S,Shim H,Wood TK

更新日期：2002-01-01 00:00:00

abstract：:The bacterium Photorhabdus luminescens is a symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora. The nematode requires the bacterium for infection of insect larvae and as a substrate for growth and reproduction. The nematodes do not grow and reproduce in insect hosts or on artificial media in the a...

journal_title：Journal of bacteriology

authors： Ciche TA,Bintrim SB,Horswill AR,Ensign JC

更新日期：2001-05-01 00:00:00