Site-directed mutagenesis reveals the importance of conserved charged residues for the transport activity of the PheP permease of Escherichia coli.

abstract：:Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid inco...

journal_title：Journal of bacteriology

authors： Hashimoto-Gotoh T,Inselburg J

更新日期：1979-08-01 00:00:00

abstract：:Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...

journal_title：Journal of bacteriology

authors： Filpula D,Fuchs JA

更新日期：1977-04-01 00:00:00

abstract：:Mycobacterium avium-Mycobacterium intracellulare complex (MAC) is the most common isolate of nontuberculous mycobacteria and causes pulmonary and extrapulmonary diseases. MAC species can be grouped into 31 serotypes by the epitopic oligosaccharide structure of the species-specific glycopeptidolipid (GPL) antigen. The ...

journal_title：Journal of bacteriology

authors： Fujiwara N,Nakata N,Naka T,Yano I,Doe M,Chatterjee D,McNeil M,Brennan PJ,Kobayashi K,Makino M,Matsumoto S,Ogura H,Maeda S

更新日期：2008-05-01 00:00:00

abstract：:Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at si...

journal_title：Journal of bacteriology

authors： Stewart P,Kersten P,Vanden Wymelenberg A,Gaskell J,Cullen D

更新日期：1992-08-01 00:00:00

abstract：:An S-adenosylhomocysteine deaminase has been isolated and purified from streptonigrin-producing Streptomyces flocculus ATCC 13257. Deamination represents the major metabolic route of S-adenosylhomocysteine in this organism. The protein was found to be monomeric with a molecular weight of 56,100 +/- 1,600. The activity...

journal_title：Journal of bacteriology

authors： Zulty JJ,Speedie MK

更新日期：1989-12-01 00:00:00

abstract：:Pseudomonas putida DOT-T1E is a solvent-resistant strain that is able to grow in the presence of high concentrations of toluene. We have cloned and sequenced the cti gene of this strain, which encodes the cis/trans isomerase, termed Cti, that catalyzes the cis-trans isomerization of esterified fatty acids in phospholi...

journal_title：Journal of bacteriology

authors： Junker F,Ramos JL

更新日期：1999-09-01 00:00:00

abstract：:gldA, the structural gene for the NAD(+)-dependent glycerol dehydrogenase, was mapped at 89.2 min on the Escherichia coli linkage map, cotransducible with, but not adjacent to, the glpFKX operon encoding the proteins for the uptake and phosphorylation of glycerol. gldA was cloned, and its position on the physical map ...

journal_title：Journal of bacteriology

authors： Truniger V,Boos W

更新日期：1994-03-01 00:00:00

abstract：:A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...

journal_title：Journal of bacteriology

authors： Gay NJ

更新日期：1984-06-01 00:00:00

abstract：:Application of in vivo expression technology (IVET) to Yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. Analysis of the translational products of both genes showed permease domain...

journal_title：Journal of bacteriology

authors： Méndez J,Reimundo P,Pérez-Pascual D,Navais R,Gómez E,Guijarro JA

更新日期：2011-02-01 00:00:00

abstract：:The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...

journal_title：Journal of bacteriology

authors： Sarsero JP,Wookey PJ,Gollnick P,Yanofsky C,Pittard AJ

更新日期：1991-05-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Immunogenic activity of a ribosomal fraction obtained from Mycobacterium tuberculosis. J. Bacteriol. 89:1291-1298. 1965.-The highly immunogenic particulate fraction obtained from mechanically ruptured cells of the H37Ra strai...

journal_title：Journal of bacteriology

authors： YOUMANS AS,YOUMANS GP

更新日期：1965-05-01 00:00:00

abstract：:Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

journal_title：Journal of bacteriology

authors： Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

更新日期：2002-07-01 00:00:00

abstract：:Brucella native haptens (NHs) extracted with hot water from smooth (S)-type B. abortus and B. melitensis were purified to high levels of serological activity and compared with the polysaccharide obtained by acid hydrolysis (PS) of the S lipopolysaccharide (S-LPS). By 13C nuclear magnetic resonance analysis, NHs showed...

journal_title：Journal of bacteriology

authors： Aragón V,Díaz R,Moreno E,Moriyón I

更新日期：1996-02-01 00:00:00

abstract：:An unusual xylose isomerase produced by Thermoanaerobacterium strain JW/SL-YS 489 was purified 28-fold to gel electrophoretic homogeneity, and the biochemical properties were determined. Its pH optimum distinguishes this enzyme from all other previously described xylose isomerases. The purified enzyme had maximal acti...

journal_title：Journal of bacteriology

authors： Liu SY,Wiegel J,Gherardini FC

更新日期：1996-10-01 00:00:00

abstract：:FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...

journal_title：Journal of bacteriology

authors： Grantcharova N,Lustig U,Flärdh K

更新日期：2005-05-01 00:00:00

abstract：:The ability of archaea to salvage cobinamide has been under question because archaeal genomes lack orthologs to the bacterial nucleoside triphosphate:5'-deoxycobinamide kinase enzyme (cobU in Salmonella enterica). The latter activity is required for cobinamide salvaging in bacteria. This paper reports evidence that ar...

journal_title：Journal of bacteriology

authors： Woodson JD,Zayas CL,Escalante-Semerena JC

更新日期：2003-12-01 00:00:00

abstract：:Glutathione and soluble thiol content were examined in a broad spectrum of bacteria. Significant soluble thiol was present in all cases. The thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. Glutathione was absent in four anerobes and one microa...

journal_title：Journal of bacteriology

authors： Fahey RC,Brown WC,Adams WB,Worsham MB

更新日期：1978-03-01 00:00:00

abstract：:The cell surfaces of several Lactobacillus species are covered by a regular layer composed of a single species of protein, the S-protein. The 43-kDa S-protein of the neotype strain Lactobacillus acidophilus ATCC 4356, which originated from the pharynx of a human, was purified. Antibodies generated against purified S-p...

journal_title：Journal of bacteriology

authors： Boot HJ,Kolen CP,van Noort JM,Pouwels PH

更新日期：1993-10-01 00:00:00

abstract：:Two novel insertion sequences, IS1312 and IS1313, were found in pTiBo542, the Ti plasmid of Agrobacterium tumefaciens strains Bo542 and A281. Nucleotide sequencing and Southern hybridization revealed that IS1312 and IS1313 are homologous to Rhizobium meliloti ISRm1 and ISRm2, respectively. IS1312, ISRm1, and another A...

journal_title：Journal of bacteriology

authors： Deng W,Gordon MP,Nester EW

更新日期：1995-05-01 00:00:00

abstract：:Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing to...

journal_title：Journal of bacteriology

authors： BANERJEA A,MUNOZ J

更新日期：1962-08-01 00:00:00

abstract：:Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control...

journal_title：Journal of bacteriology

authors： Nash HA,Robertson CA,Flamm E,Weisberg RA,Miller HI

更新日期：1987-09-01 00:00:00

abstract：:Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...

journal_title：Journal of bacteriology

authors： HARPER MK,CURRAN HR,PALLANSCH MJ

更新日期：1964-11-01 00:00:00

abstract：:Mutations in the tol-pal genes induce pleiotropic effects such as release of periplasmic proteins into the extracellular medium and hypersensitivity to drugs and detergents. Other outer membrane defective strains such as tolC, lpp, and rfa mutations are also altered in their outer membrane permeability. In this study,...

journal_title：Journal of bacteriology

authors： Bernadac A,Gavioli M,Lazzaroni JC,Raina S,Lloubès R

更新日期：1998-09-01 00:00:00

abstract：:Staphylococcus pseudintermedius is a member of the coagulase-positive staphylococci and is the commonest cause of canine pyoderma. We report the first genome sequence of S. pseudintermedius, which shows the presence of numerous virulence factors akin to those of the related human pathogen Staphylococcus aureus. ...

journal_title：Journal of bacteriology

authors： Tse H,Tsoi HW,Leung SP,Urquhart IJ,Lau SK,Woo PC,Yuen KY

更新日期：2011-04-01 00:00:00

abstract：:Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...

journal_title：Journal of bacteriology

authors： Zhao N,Bai Y,Zhao XQ,Yang ZY,Bai FW

更新日期：2012-12-01 00:00:00

abstract：:Salmonella typhimurium LT2 normally expresses two acetohydroxy acid synthases (AHAS I and AHAS II). The function of AHAS I in this organism was unclear, since AHAS I-deficient (ilvBN) mutants of LT2 grew well on glucose or succinate minimal media, whereas AHAS II-deficient (ilvGM) mutants requried isoleucine for norma...

journal_title：Journal of bacteriology

authors： Dailey FE,Cronan JE Jr,Maloy SR

更新日期：1987-02-01 00:00:00

abstract：:The production and regeneration of bacterial protoplasts promoted the loss of three different plasmid-specified traits in Streptococcus lactis subsp. diacetylactis strains. The loss of five different plasmids, including small multicopy molecules, was readily detected in Streptococcus lactis 712 by screening lysates of...

journal_title：Journal of bacteriology

authors： Gasson MJ

更新日期：1983-04-01 00:00:00

abstract：:Sulfhydryl oxidants and stabilizers caused changes demonstrating the sulfhydryl content of beta-galactosidase for Streptococcus lactis 7962. Ammonium sulfate (0.85 m) rendered the enzyme insensitive to the oxidants. Titrations revealed 11.5 moles of sulfhydryl per mole of enzyme. ...

journal_title：Journal of bacteriology

authors： McFeters GA,Sandine WE,Elliker PR

更新日期：1971-10-01 00:00:00

abstract：:We report the draft genome sequence of Paenisporosarcina sp. strain TG-20, which is 4.12 Mb in size and consists of 4,071 protein-coding genes and 76 RNA genes. The genome sequence of Paenisporosarcina sp. TG-20 may provide useful information about molecular adaptations that enhance survival in icy subsurface environm...

journal_title：Journal of bacteriology

authors： Lee JH,Koh HY,Lee SG,Doyle S,Christner BC,Kim HJ

更新日期：2012-12-01 00:00:00

abstract：:In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. Th...

journal_title：Journal of bacteriology

authors： Oliver EJ,Mortlock RP

更新日期：1971-10-01 00:00:00