ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.

Abstract:

:Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid incompatibility lies between base pairs -185 and -684; (ii) the integrity of at least part of the region of ColE1 DNA between base pairs -185 and -572 is essential for the expression of ColE1 incompatibility; (iii) the expression of incompatibility is independent of the ability of the ColE1 genome to replicate autonomously; (iv) plasmid incompatibility is affected by plasmid copy number; and (v) ColE1 plasmid-mediated DNA replication of the lambda phage-ColE1 chimera lambda imm434 Oam29 Pam3 ColE1 is inhibited by ColE1-incompatible but not by ColE1-compatible plasmids.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hashimoto-Gotoh T,Inselburg J

doi

10.1128/JB.139.2.608-619.1979

subject

Has Abstract

pub_date

1979-08-01 00:00:00

pages

608-19

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

139

pub_type

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