Abstract:
:Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid incompatibility lies between base pairs -185 and -684; (ii) the integrity of at least part of the region of ColE1 DNA between base pairs -185 and -572 is essential for the expression of ColE1 incompatibility; (iii) the expression of incompatibility is independent of the ability of the ColE1 genome to replicate autonomously; (iv) plasmid incompatibility is affected by plasmid copy number; and (v) ColE1 plasmid-mediated DNA replication of the lambda phage-ColE1 chimera lambda imm434 Oam29 Pam3 ColE1 is inhibited by ColE1-incompatible but not by ColE1-compatible plasmids.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hashimoto-Gotoh T,Inselburg Jdoi
10.1128/JB.139.2.608-619.1979subject
Has Abstractpub_date
1979-08-01 00:00:00pages
608-19issue
2eissn
0021-9193issn
1098-5530journal_volume
139pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6479-6487.1992
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更新日期:1966-08-01 00:00:00
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更新日期:1998-09-01 00:00:00
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更新日期:1982-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
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更新日期:1995-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1990-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.01120-07
更新日期:2008-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1403-1415.1964
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更新日期:2001-09-01 00:00:00
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更新日期:1999-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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更新日期:1999-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-08-01 00:00:00
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更新日期:1992-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2008-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2018-08-24 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
abstract::Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...
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pub_type: 杂志文章
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更新日期:1984-05-01 00:00:00